A mutagenic PCR identifies isolates of Borrelia garinii responsible for Lyme borreliosis

FEMS Microbiology Letters

Volumn 191, Issue 2, 2000, Pages 199-203

  Mueller, Célia ^a   Hutter, Pierre ^b   Sahli, Roland ^c   Bretz, Anne Gabrielle ^a   Toutoungi, Lina N ^a   Péter, Olivier ^a  

^a Institut Central des Hopitaux Valaisans   (Switzerland)

^b Pathol Inst Ctrl Hop V   (Switzerland)

^c LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

Author keywords

Borrelia garinii; Lyme borreliosis; Mutagenic polymerase chain reaction; Phosphocarrier protein HPR

Indexed keywords

AMINO ACID; BACTERIAL PROTEIN;

ARTICLE; BORRELIA GARINII; CONTROLLED STUDY; DIAGNOSTIC TEST; DIAGNOSTIC VALUE; DNA SEQUENCE; GENE MUTATION; LYME DISEASE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ANIMAL; BASE SEQUENCE; BORRELIA; CLONING, MOLECULAR; GENES, BACTERIAL; HUMAN; LYME DISEASE; MOLECULAR SEQUENCE DATA; MUTATION; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE SYSTEM; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SUPPORT, NON-U.S. GOV'T;

BORRELIA BURGDORFERI; BORRELIA GARINII;

EID: 0034668295     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00390-6     Document Type: Article

References (13)

1. Baranton G., Postic D., Saint Girons I., Boerlin P., Piffaretti J.C., Assous M., Grimont P.A.D. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int. J. Syst. Bacteriol. 42:1992;378-383.
2. Assous M.V., Postic D., Paul G., Névot P., Baranton G. Western blot analysis of sera from Lyme borreliosis patients according to the genomic species of the Borrelia strains used as antigens. Eur. J. Clin. Microbiol. Infect. Dis. 12:1993;261-268.
3. Anthonissen F.M., De Kesel M., Hoet P.P., Bigaignon G.H. Evidence for the involvement of different genospecies of Borrelia in the clinical outcome of Lyme disease in Belgium. Res. Microbiol. 145:1994;327-331.
4. Péter O., Bretz A.G., Postic D., Dayer E. Association of distinct species of Borrelia burgdorferi sensu lato with neuroborreliosis in Switzerland. Clin. Microbiol. Infect. 3:1997;423-431.
5. Wilske B., Jauris-Heipke S., Lobentanzer R., et al. Phenotypic analysis of outer surface protein C (OspC) of Borrelia burgdorferi sensu lato by monoclonal antibodies: relationship to genospecies and OspA serotype. J. Clin. Microbiol. 33:1995;103-109.
6. Postic D., Assous M.V., Grimont P.A.D., Baranton G. Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int. J. Syst. Bacteriol. 44:1994;743-752.
7. Hubalek Z., Halouzka J. Distribution of Borrelia burgdorferi sensu lato genomic groups in Europe, a review. Eur. J. Epidemiol. 13:1997;951-957.
8. Saint-Girons I., Gern L., Gray J.S., et al. Identification of Borrelia burgdorferi sensu lato species in Europe. Zbl. Bakteriol. 287:1998;190-195.
9. Olsen B., Duffy D.C., Jaenson T.G.T., Gylfe A., Bonnedahl J., Bergström S. Transhemispheric exchange of Lyme disease spirochetes by seabirds. J. Clin. Microbiol. 33:1995;3270-3274.
10. Humair P.-F., Péter O., Wallich R., Gern L. Strain variation of Lyme disease spirochete isolated from Ixodes ricinus ticks and rodents collected in two endemic areas in Switzerland. J. Med. Entomol. 32:1995;433-438.
11. Barbour A.G. Isolation and cultivation of Lyme disease spirochetes. Yale J. Biol. Med. 57:1984;521-525.
12. Maniatis, T.E., Fritsch, E.F. and Sambrook, J. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
13. Fraser C.M., Casjens S., Huang W.M., et al. Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature. 390:1997;580-586.