Enhancement of catalytic efficiency of enzymes through exposure to anhydrous organic solvent at 70°C. Three-dimensional structure of a treated serine proteinase at 2.2 Å resolution

Proteins: Structure, Function and Genetics

Volumn 39, Issue 3, 2000, Pages 226-234

  Gupta, M N ^a   Tyagi, Renu ^a   Sharma, Sujata ^b   Karthikeyan, S ^b   Singh, T P ^b  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

^b ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Anhydrous organic solvents; Crystal structure; Enhanced activity; Proteinase K; Stability

Indexed keywords

ORGANIC SOLVENT; SERINE PROTEINASE;

ARTICLE; BINDING SITE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME STRUCTURE; HYDROGEN BOND; MOLECULAR STABILITY; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

ACETONITRILES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; DIMETHYLFORMAMIDE; ENDOPEPTIDASE K; HEAT; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; SERINE ENDOPEPTIDASES; SOLVENTS; STRUCTURE-ACTIVITY RELATIONSHIP; TOLUENE;

TRITICUM AESTIVUM;

EID: 0034657542     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0134(20000515)39:3<226::AID-PROT50>3.0.CO;2-Y     Document Type: Article

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