A sensitive assay for phosphoinositide phosphatases

Analytical Biochemistry

Volumn 279, Issue 2, 2000, Pages 248-250

  Maehama, Tomohiko ^a   Taylor, Gregory S ^a   Slama, James T ^b   Dixon, Jack E ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b MEDICAL COLLEGE OF OHIO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

PHOSPHOINOSITIDE PHOSPHATASE; SENSITIVE ASSAY;

CHEMISTRY;

MALACHITE GREEN; PHOSPHATASE; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE 3 PHOSPHATASE; PHOSPHOINOSITIDE PHOSPHATASE; PROTEIN SAC1P; UNCLASSIFIED DRUG;

ACCURACY; ANALYTIC METHOD; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; NONHUMAN; NOTE; PRIORITY JOURNAL; PROTEIN EXPRESSION; VALIDATION PROCESS;

SIPROETA STELENES;

EID: 0034654395     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4497     Document Type: Article

References (10)

1. Cho H. J., Ramer S. E., Itoh M., Winkler D. G., Kitas E., Bannwarth W., Burn P., Saito H., Walsh C. T. Purification and characterization of a soluble catalytic fragment of the human transmembrane leukocyte antigen related (LAR) protein tyrosine phosphatase from an Escherichia coli expression system. Biochemistry. 30:1991;6210-6216.
2. Harder K. W., Owen P., Wong L. K., Aebersold R., Clark-Lewis I., Jirik F. R. Characterization and kinetic analysis of the intracellular domain of human protein tyrosine phosphatase beta (HPTP beta) using synthetic phosphopeptides. Biochem. J. 298:1994;395-401.
3. Martin B., Pallen C. J., Wang J. H., Graves D. J. Use of fluorinated tyrosine phosphates to probe the substrate specificity of the low molecular weight phosphatase activity of calcineurin. J. Biol. Chem. 260:1985;14932-14937.
4. Hess H. H., Derr J. E. Assay of inorganic and organic phosphorus in the 0.1-5 nanomole range. Anal. Biochem. 63:1975;607-613.
5. Lanzetta P. A., Alvarez L. J., Reinach P. S., Candia O. A. An improved assay for nanomole amounts of inorganic phosphate. Anal. Biochem. 100:1979;95-97.
6. Maehama T., Dixon J. E. The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate. J. Biol. Chem. 273:1998;13375-13378.
7. Maehama T., Dixon J. E. PTEN: A tumour suppressor that functions as a phospholipid phosphatase. Trends Cell Biol. 9:1999;125-128.
8. Myers M. P., Pass I., Batty I. H., Van der Kaay J., Stolarov J. P., Hemmings B. A., Wigler M. H., Downes C. P., Tonks N. K. The lipid phosphatase activity of PTEN is critical for its tumor suppressor function. Proc. Natl. Acad. Sci. USA. 95:1998;13513-13518.
9. Stambolic V., Suzuki A., de la Pompa J. L., Brothers G. M., Mirtsos C., Sasaki T., Ruland J., Penninger J. M., Siderovski D. P., Mak T. W. Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN. Cell. 95:1998;29-39.
10. Guo S., Stolz L. E., Lemrow S. M., York J. D. SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases. J. Biol. Chem. 274:1999;12990-12995.