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Volumn 122, Issue 10, 2000, Pages 2393-2394

The HNO adduct of myoglobin: Synthesis and characterization [12]

Author keywords

[No Author keywords available]

Indexed keywords

MYOGLOBIN; NITRIC OXIDE;

EID: 0034654071     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja994079n     Document Type: Letter
Times cited : (102)

References (45)
  • 26
    • 0343156307 scopus 로고    scopus 로고
    • note
    • 13c The solution turned light blue immediately and gradually changed to purple. An excess of fresh Cr(EDTA) was added to the nitrosyl myoglobin solution at pH 10.0 to give essentially quantitative production of 2, by UV-vis. The reduced protein was repurified through a G-25 column, and the resulting solution was concentrated down by centrifugation before spectral measurements. To generate solutions of 2 at different pH, the buffer was replaced by dilution and centrifugation with desired buffer solution.
  • 27
    • 0029792561 scopus 로고    scopus 로고
    • Packer, L., Ed.; Academic Press: San Diego
    • (b) Arnold, E. V.; Bohle, D. S. in Methods in Enzymologv, Packer, L., Ed.; Academic Press: San Diego, 1996; Vol. 268, Part B, pp 41-55.
    • (1996) Methods in Enzymologv , vol.268 , Issue.PART B , pp. 41-55
    • Arnold, E.V.1    Bohle, D.S.2
  • 29
    • 0342286729 scopus 로고    scopus 로고
    • note
    • 14b For the electrochemical reduction of nitrosyl myoglobin, monomethyl 4,4′-bipyridine was used as the mediator. A three-neck UV-vis cuvette was employed with a gold working electrode, an SCE reference electrode, and a Pt counter electrode. The solution was degassed with nitrogen, and both nitrosyl myoglobin and monomethyl 4,4′-bipyridine were added. As a -750 mV vs NHE potential was imposed, the spectra change was followed by UV-vis.
  • 31
    • 0342721815 scopus 로고    scopus 로고
    • note
    • A methyl viologen dichloride (Aldrich) solution was made by dissolving 3 mg of methyl viologen in pH 10.0 buffer, and then it was added by aliquots to the solution of 2 in a gastight UV-vis cell. Resulting spectra are given in the Supporting Information.
  • 33
    • 0343591792 scopus 로고    scopus 로고
    • note
    • 4Cl (Oxford).
  • 44
    • 0342286726 scopus 로고    scopus 로고
    • note
    • 2, an H-atom added, and the protein adduct allowed to minimize from several different starting conformations, resulting in the shown active-site structure.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.