Identification of iron-regulated genes of Helicobacter pylori by a modified Fur titration assay (FURTA-Hp)

FEMS Microbiology Letters

Volumn 184, Issue 2, 2000, Pages 225-229

  Fassbinder, Frank ^a   Van Vliet, Arnoud H M ^b   Gimmel, Verena ^a   Kusters, Johannes G ^b   Kist, Manfred ^a   Bereswill, Stefan ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

Ferric uptake regulator; Fur regulon; Iron regulation; Iron uptake

Indexed keywords

FERRIC CITRATE; IRON REGULATORY FACTOR;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; HELICOBACTER PYLORI; IRON METABOLISM; IRON TRANSPORT; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CARRIER PROTEINS; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; HELICOBACTER PYLORI; IRON; MOLECULAR SEQUENCE DATA; RECEPTORS, CELL SURFACE; RECOMBINANT PROTEINS; REGULON; REPRESSOR PROTEINS; REPRODUCIBILITY OF RESULTS; RIBOFLAVIN; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI; HELICOBACTER PYLORI;

EID: 0034653295     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00054-9     Document Type: Article

References (16)

1. Litwin C.M., Calderwood S.B. Role of iron in regulation of virulence genes. Clin. Microbiol. Rev. 6:1993;137-149.
2. Hantke K. Selection procedure for deregulated iron transport mutants (fur) in Escherichia coli K12-fur not only affects iron-metabolism. Mol. Gen. Genet. 210:1987;135-139.
3. Stojiljkovic I., Baumler A.J., Hantke K. Fur regulon in gram-negative bacteria. Identification and characterization of new iron-regulated Escherichia coli genes by a fur titration assay. J. Mol. Biol. 236:1994;531-545.
4. Husson M.O., Legrand D., Spik G., Leclerc H. Iron acquisition by Helicobacter pylori: importance of human lactoferrin. Infect. Immun. 61:1993;2694-2697.
5. Worst D.J., Otto B.R., de Graaff J. Iron-repressible outer membrane proteins of Helicobacter pylori involved in heme uptake. Infect. Immun. 63:1995;4161-4165.
6. Worst D.J., Sparrius M., Kuipers E.J., Kusters J.G., de Graaff J. Human serum antibody response against iron-repressible outer membrane proteins of Helicobacter pylori. FEMS Microbiol. Lett. 144:1996;29-32.
7. Worst D.J., Gerrits M.M., Vandenbroucke-Grauls C.M.J.E., Kusters J.G. Helicobacter pylori ribBA-mediated riboflavin production is involved in iron acquisition. J. Bacteriol. 180:1998;1473-1479.
8. Tomb J.F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.P., Gill S., Dougherty B.A., Nelson K., Quackenbush J., Zhou L., Kirkness E.F., Peterson S., Loftus B., Richardson D., Dodson R., Khalak H.G., Glodek A., McKenney K., Fitzgerald L.M., Lee N., Adams M.D., Venter J.C. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature. 388:1997;539-547.
9. Bereswill S., Lichte F., Vey T., Fassbinder F., Kist M. Cloning and characterization of the fur gene from Helicobacter pylori. FEMS Microbiol. Lett. 159:1998;193-200.
10. Bereswill S., Waidner U., Odenbreit S., Lichte F., Fassbinder F., Bode G., Kist M. Structural, functional and mutational analysis of the pfr gene encoding a ferritin from Helicobacter pylori. Microbiology. 144:1998;2505-2516.
11. Beier D., Spohn G., Rappuoli R., Scarlato V. Identification and characterization of an operon of Helicobacter pylori that is involved in motility and stress adaptation. J. Bacteriol. 179:1997;4676-4683.
12. Bereswill S., Lichte F., Greiner S., Waidner B., Fassbinder F., Kist M. The Ferric Uptake Regulator (Fur) homologue of Helicobacter pylori: functional analysis of the coding gene and controlled production of the recombinant protein in Escherichia coli. Med. Microbiol. Immunol. 181:1999;31-40.
13. van Vliet A.H.M., Wooldridge K.G., Ketley J.M. Iron-responsive gene regulation in a Campylobacter jejuni fur mutant. J. Bacteriol. 180:1998;5291-5298.
14. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (Eds.) (1992) Short Protocols in Molecular Biology, 2nd edn. John Wiley and Sons, New York.
15. Geider K. DNA cloning vectors utilizing replication functions of the filamentous phages of Escherichia coli. J. Gen. Virol. 67:1986;2287-2303.
16. Tsolis R.M., Baumler A.J., Stojiljkovic I., Heffron F. Fur regulon of Salmonella typhimurium: identification of new iron-regulated genes. J. Bacteriol. 177:1995;4628-4637.