A novel perfusion system for animal cell cultures by two step sequential sedimentation

Journal of Biotechnology

Volumn 79, Issue 1, 2000, Pages 1-11

  Wen, Zhi You ^a,b   Teng, Xiao Wei ^b   Chen, Feng ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

^b EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Airlift bioreactor; Gravity sedimentation; Hybridoma cells; Perfusion culture

Indexed keywords

MONOCLONAL ANTIBODY;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL CULTURE; CELL SEPARATION; CONTROLLED STUDY; GRAVITY; HYBRIDOMA; NONHUMAN; PERFUSION; PRIORITY JOURNAL; RAT; THEORY;

ANIMALS; ANTIBODIES, MONOCLONAL; BIOREACTORS; CELL COUNT; CELL CULTURE TECHNIQUES; CELL LINE; CELL SEPARATION; CELL SURVIVAL; GRAVITATION; HYBRIDOMAS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN KAPPA-CHAINS; MICE; PERFUSION; RATS;

ANIMALIA;

EID: 0034647042     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(00)00219-4     Document Type: Article

References (30)

1. Apelman S. Separation of animal cells in continuous cell culture systems. Murakami H., Shirahata S., Tachibana H. Animal Cell Technology: Basic and Applied Aspects. 1992;149-154 Kluwer Academic Publishers, Dordrecht.
2. Arai T., Yokoyama S., Tokashiki M. 50 l scale perfusion culture of hybridoma cells by gravitational settling for cell separation. Kaminogawa S., Ametani A., Hachimura S. Animal Cell Technology: Basic and Applied Aspects. 1993;341-346 Kluwer Academic Publishers, Dordrecht.
3. Avgerinos G.C., Drapeau D., Socolow J.S., Mao J., Hsiao K., Broeze R.J. Spin filter perfusion system for high density cell culture: production of recombinant urinary type plasminogen activator in CHO cells. Bio/Technology. 8:1990;54-58.
4. Batt B.C., Davis R.H., Kompala D.S. Inclined sedimentation for selective retention of viable hybridomas in a continuous suspension bioreactor. Biotechnol. Prog. 6:1990;458-464.
5. Brennan A.J., Shevitz J., Macmillan J.D. A perfusion system for antibody production by shear sensitive hybridoma cells in a stirred reactor. Biotechnol. Tech. 1:1987;169-174.
6. Büntemeyer H., Böhme C., Lehmann J. Evaluation of membranes for use in on-line cell separation during mammalian cell perfusion process. Cytotechnology. 15:1994;243-251.
7. Docoslis A., Kalogerakis N., Behie L.A., Kaler V.I.S. A novel dielectrophoresis-based device for the selective retention of viable cells in cell culture media. Biotechnol. Bioeng. 54:1997;239-250.
8. Freshney R.I. Culture of Animal Cells. 1994;179 Wiley, New York.
9. Hamamoto K., Ishimaru K., Tokashiki M. Perfusion culture of hybridoma cells using a centrifuge to separate cells from culture mixture. J. Ferment. Bioeng. 67:1989;190-194.
10. Hülscner M., Scheibler U., Onken U. Selective recycle of viable animal cells by coupling of airlift reactor cell settler. Biotechnol. Bioeng. 39:1992;442-446.
11. Jáger V. High density perfusion culture of animal cells using a novel continuous flow centrifuge. Murakami H., Shirahata S., Tachibana H. Animal Cell Technology: Basic and Applied Aspects. 1992;209-216 Kluwer Academic Publishers, Dordrecht.
12. Johnson M., Lanthier S., Massie B., Lefebvre G., Kamen A.A. Use of the centritech lab centrifuge for perfusion culture of hybridoma cells in protein-free medium. Biotechnol. Prog. 12:1996;855-864.
13. Kitano K., Shintani Y., Ichimori Y., Tsukamoto K., Sasai S., Kida M. Production of human monoclonal antibody by heterohybridomas. Appl. Microbiol. Biotechnol. 24:1986;282-286.
14. Knaack C., André G., Chavarie C. Conical bioreactor with internal lamella settler for perfusion culture of suspension cells. Spier R.E., Griffiths J.B., Berthold W. Animal Cell Technology: Products of Today, Prospects for Tomorrow. 1994;230-233 Butterworth-Heinemann, Oxford.
15. Mercille S., Johnson M., Lemieux R., Massie B. Filtration-based perfusion of hybridoma cultures in protein-free medium: reduction of membrane fouling by medium supplementation with DNase I. Biotechnol. Bioeng. 43:1994;833-846.
16. Ohashi R., Mochizuki E., Kamoshita Y., Suzuki T. High-level expression of the methanol-inducible β-galactosidase gene by perfusion culture of recombinant Pichia pastoris using a shaken ceramic membrane flask. J. Ferment. Bioeng. 86:1998;44-48.
17. Ozturk S.S., Thrift J.C., Blackie J.D., Naveh D. Real-time monitoring and control of glucose and lactate concentration in a mammalian cell perfusion reactor. Biotechnol. Bioeng. 53:1997;372-378.
18. Searles J.A., Todd P., Kompala D.S. Viable cell recycle with an inclined settler in the perfusion culture of suspended recombinant Chinese Hamster Ovary cells. Biotechnol. Prog. 10:1994;198-206.
19. Stevens J., Eickel S., Onken U. Lamellar clarifier - a new device for animal cell retention in perfusion culture systems. Spier R.E., Griffiths J.B., Berthold W. Animal Cell Technology: Products of Today, Prospects for Tomorrow. 1994;234-239 Butterworth-Heinemann, Oxford.
20. Su W.W., He B.J., Liang H., Sun S. A perfusion air-lift bioreactor for high density plant cell cultivation and secreted protein production. J. Biotechnol. 50:1996;225-233.
21. Su W.W., He B.J. Secreted enzyme production by fungal pellets in a perfusion bioreactor. J. Biotechnol. 54:1997;43-52.
22. Takazawa Y., Tokashiki M., Murakami H., Yamada K., Omura H. High-density culture of mouse-human hybridoma in serum-free defined medium. Biotechnol. Bioeng. 31:1988;168-172.
23. Thompson K.J., Wilson J.S. A compact gravitational settling device for cell retention. Spier R.E., Griffiths J.B., Berthold W. Animal Cell Technology: Products of Today, Prospects for Tomorrow. 1994;227-229 Butterworth-Heinemann, Oxford.
24. Tokashiki M., Arai T. High density culture of hybridoma cells using a perfusion culture apparatus with multi-settling zone to separate cells from the culture medium. Cytotechnology. 2:1989;5-8.
25. Tokashiki M., Arai T. High density culture of hybridoma cells using a perfusion culture apparatus with multi-settling zone. Spier R.E., Griffiths J.B., Meignier B. Production of Biologicals from Animal Cells in Culture. 1991;467-469 Butterworth-Heinemann, Oxford.
26. Tokashiki M., Arai T., Hamamoto K., Ishimaru K. High density culture of hybridoma cells using a perfusion culture vessel with an external centrifuge. Cytotechnology. 3:1990;239-244.
27. Tokashiki M., Takamatsu H. Perfusion culture apparatus for suspended mammalian cells. Cytotechnology. 13:1993;149-159.
28. Velez D., Miller L., Macmillan J.D. Use of tangential flow filtration in perfusion propagation of hybridoma cells for propagation of monoclonal antibodies. Biotechnol. Bioeng. 33:1989;938-940.
29. Vogel J.H., Kroner K.-H. Controlled shear filtration: a novel technique for animal cell separation. Biotechnol. Bioeng. 63:1999;663-674.
30. Zhang J., Collins A., Chen M., Knyazev I., Gentz R. High-density perfusion culture of insect cells with a BioSep ultrasonic filter. Biotechnol. Bioeng. 59:1997;351-359.