High-level expression of the methanol-inducible β-galactosidase gene by perfusion culture of recombinant Pichia pastoris using a shaken ceramic membrane flask

Journal of Fermentation and Bioengineering

Volumn 86, Issue 1, 1998, Pages 44-48

  Ohashi, Ryo ^a   Mochizuki, Eiko ^b   Kamoshita, Yuya ^a   Suzuki, Takahiro ^a  

^a TOKYO UNIVERSITY OF SCIENCE   (Japan)

^b Development and Medical Affairs Division   (Japan)

Author keywords

Methanol inducible gene; Perfusion culture; Pichia pastoris; SCM flask; galactosidase

Indexed keywords

CATALYST ACTIVITY; CERAMIC MATERIALS; COMPOSITION EFFECTS; ENZYMES; GLYCEROL; MEMBRANES; METHANOL; PLANT CELL CULTURE; YEAST;

GALACTOSIDASE; PERFUSION CULTURE; SHAKEN CERAMIC MEMBRANE (SCM) FLASKS;

GENETIC ENGINEERING;

BETA GALACTOSIDASE; GLYCEROL; METHANOL;

ARTICLE; CERAMICS; CONTROLLED STUDY; ENDOMYCETALES; ENZYME INDUCTION; FUNGUS CULTURE; NONHUMAN; PERFUSION; PROTEIN EXPRESSION;

FUNGI; PICHIA PASTORIS; SACCHAROMYCETALES;

EID: 0031822160     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0922-338X(98)80032-9     Document Type: Article

References (7)

1. Suzuki, T., Kamoshita, Y., and Ohashi, R.: A dense cell culture system for microorganisms using a shake flask incorporating a porous ceramic filter. J. Ferment. Bioeng., 84, 133-137 (1997).
2. Kamoshita, Y., Ohashi, R., and Suzuki, T.: A dense cell culture system for aerobic microorganisms using a shaken ceramic membrane flask with surface aeration. J. Ferment. Bioeng., 85, 218-222 (1998).
3. Gregg, J. M., Tschopp, J. F., Stillman, C., Siegel, R., Akong, M., Graig, W. S., Buckholtz, R. G., Madden, K. R., Kellaris, P. A., Davis, G. R., Smiley, B. L., Gruze, J., Torregrossa, R., Velicelebio, G., and Thill, G. P.: High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast, Pichia pastoris. Bio/Technology, 5, 479-485 (1987).
4. Sreekrishna, K., Nelles, L., Potenz, R., Cruze, J., Mazzaferro, P., Fish, W., Fuke, M., Holden, K., Phelps, D., Wood, P., and Parker, K.: High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast, Pichia pastoris. Biochemistry, 28, 4117-4125 (1989).
5. Tschopp, J. F., Sverlow, G., Kosson, R., Graig, W., and Grinna, L.: High-level selection of glycosylated invertase in the methylotrophic yeast, Pichia postoris. Bio/Technology, 5, 1305-1308 (1987).
6. Tschopp, J. F., Brust, P. F. Cregg, J. M., Stillman, C. A., and Gingeras, T. R.: Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucl. Acid Res., 15, 3859-3876 (1987).
7. Suzuki T., Yamane, T., and Shimizu, S.: Phenomenological background and some preliminary trials of automated substrate supply in pH-stat fed-batch culture utilizing a set-point of high limit. J. Ferment. Bioeng., 69, 292-297 (1990).