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0001191585
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0030932902
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Nocek, J. M.; Sishta, B. P.; Cameron, J. C: Mauk, A. G.; Hoffman, B. M. J. Am. Chem. Soc. 1997, 119, 2146-2155.
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Hoffman, B.M.5
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7
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33646286162
-
-
The reconstitution procedures for the preparation of ZnDMb and ZnD(dme)Mb are slightly different. ZnDMb was prepared by adding 10% NaOH solution containing ZnD porphyrin into the apoMb solution. ZnD(dme)Mb was prepared by adding DMSO/CHiOH(v/v 3:1) containing ZnD(dme) porphyrin into the apoMb solution; the final concentration of the organic solvent was kept below 8% to prevent denaturation
-
The reconstitution procedures for the preparation of ZnDMb and ZnD(dme)Mb are slightly different. ZnDMb was prepared by adding 10% NaOH solution containing ZnD porphyrin into the apoMb solution. ZnD(dme)Mb was prepared by adding DMSO/CHiOH(v/v 3:1) containing ZnD(dme) porphyrin into the apoMb solution; the final concentration of the organic solvent was kept below 8% to prevent denaturation.
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8
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0031056708
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Hunter, C. L.; Lloyd, E.; Eltis, L. D.; Rafferty, S. P.; Lee, H.; Smith. M.; Mauk, A. G. Biochemistry 1997, 36, 1010-1017.
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Lee, H.5
Smith, M.6
Mauk, A.G.7
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9
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0000596542
-
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La Mar, G. N.; Emerson, S. D.; Lecomte, J. T. J.; Pande, U.; Smith, K. M.; Craig, G. W.; Retires, L. A. J. Am. Chem. Soc. 1986, 108, 5568-5573.
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La Mar, G.N.1
Emerson, S.D.2
Lecomte, J.T.J.3
Pande, U.4
Smith, K.M.5
Craig, G.W.6
Retires, L.A.7
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10
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0032554615
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Neya, S.; Funasaki, N.; Igarashi. N.; Ikezaki, A.; Sato, T.; Imai, K.; Tanaka, N. Biochemistry 1998, 37, 5487-5493.
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Tanaka, N.7
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11
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0025335197
-
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Funk, W. D.; Lo, T. P.; Mauk, M. R.; Brayer, G. D.; MacGillivray, R. T. A.; Mauk, A. G. Biochemistry 1990, 29, 5500-5508.
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Funk, W.D.1
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Mauk, M.R.3
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MacGillivray, R.T.A.5
Mauk, A.G.6
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12
-
-
33646294740
-
-
5 and a minor component (2-5%) that does not and is hence ignored
-
5 and a minor component (2-5%) that does not and is hence ignored.
-
-
-
-
13
-
-
33646282071
-
-
The transient absorption spectrum at times longer than the triplet decay has a feature around 670 nm assigned lo the radical cation of ZnDMb/ZnD(dme)Mh, along with one at 562 nm attributed to reduced cyt b$
-
( 13) The transient absorption spectrum at times longer than the triplet decay has a feature around 670 nm assigned lo the radical cation of ZnDMb/ZnD(dme)Mh, along with one at 562 nm attributed to reduced cyt b$.
-
-
-
-
14
-
-
33646282922
-
-
3+P Forster energy transfer depends on the emission spectrum of the energy donor, which is the same for ZnDMb and ZnD(dme)Mb (Turro, N. J. Modem Molecular Photochemistry; Benjamin/ Cummings Publishing Co.: Menlo Park, CA, 1978). Consequently any increase in energy transfer with esterificalion must come from a charge in the distribution of binding geometries, as discussed below
-
3+P Forster energy transfer depends on the emission spectrum of the energy donor, which is the same for ZnDMb and ZnD(dme)Mb (Turro, N. J. Modem Molecular Photochemistry; Benjamin/ Cummings Publishing Co.: Menlo Park, CA, 1978). Consequently any increase in energy transfer with esterificalion must come from a charge in the distribution of binding geometries, as discussed below.
-
-
-
-
15
-
-
0000412653
-
-
McLendon, G.; Zhang, Q.; Wallin, S. A.: Miller, R. M.; Billstone, V.; Spears. K. G.; Huffman, B. M. J. Am. Chem. Soc. 1993, 115, 3665-3669.
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McLendon, G.1
Zhang, Q.2
Wallin, S.A.3
Miller, R.M.4
Billstone, V.5
Spears, K.G.6
Huffman, B.M.7
-
16
-
-
33646273003
-
-
Binding constants were measured on a MicroCal isothermal titration calorimeter; experimental conditions were the same as in the photolysis experiments except the protein concentration is in the mM range
-
Binding constants were measured on a MicroCal isothermal titration calorimeter; experimental conditions were the same as in the photolysis experiments except the protein concentration is in the mM range.
-
-
-
-
17
-
-
0001102428
-
-
Nocek, J. M.; Zhou, J. S.; De Forest, S.; Priyadarshy, S.: Beratan, D. N.; Onuchic. J. N.; Hoffman. B. M. Chem. Rev. 1996, 96, 2459-2489.
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Nocek, J.M.1
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De Forest, S.3
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Beratan, D.N.5
Onuchic, J.N.6
Hoffman, B.M.7
-
21
-
-
0345330736
-
-
5 and intraprotein ET reaction of rutheniummodified ZnMb (Winkler. J. R.; Gray, H. B. Chem. Re,. 1992. 92. 369-379.
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(1992)
Chem. Re,.
, vol.92
, pp. 369-379
-
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Winkler, J.R.1
Gray, H.B.2
-
23
-
-
0000731243
-
-
Dixon. D. W.; Hong, X.; Woehler, S. E.; Muuk, A. G.; Sishta. B. P. J. Am. Chem. Sm. 1990, 112, 1082-1088).
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-
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Dixon, D.W.1
Hong, X.2
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Muuk, A.G.4
Sishta, B.P.5
-
24
-
-
33646285224
-
-
note
-
This is consistent with the observation that neutralisation has no effect on the steady-state fluorescence, energy-transfer quenching titration, which should be dominated by the majority conformations. Time-resolved fluorescence quenching studies will be performed to give further insight into this issue.
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