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Volumn 122, Issue 14, 2000, Pages 3552-3553

Electrostatic control of electron transfer between myoglobin and cytochrome b5: Effect of methylating the heme propionates of Zn-myoglobin [16]

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME B; MYOGLOBIN; ZINC;

EID: 0034640035     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja993951p     Document Type: Letter
Times cited : (33)

References (27)
  • 7
    • 33646286162 scopus 로고    scopus 로고
    • The reconstitution procedures for the preparation of ZnDMb and ZnD(dme)Mb are slightly different. ZnDMb was prepared by adding 10% NaOH solution containing ZnD porphyrin into the apoMb solution. ZnD(dme)Mb was prepared by adding DMSO/CHiOH(v/v 3:1) containing ZnD(dme) porphyrin into the apoMb solution; the final concentration of the organic solvent was kept below 8% to prevent denaturation
    • The reconstitution procedures for the preparation of ZnDMb and ZnD(dme)Mb are slightly different. ZnDMb was prepared by adding 10% NaOH solution containing ZnD porphyrin into the apoMb solution. ZnD(dme)Mb was prepared by adding DMSO/CHiOH(v/v 3:1) containing ZnD(dme) porphyrin into the apoMb solution; the final concentration of the organic solvent was kept below 8% to prevent denaturation.
  • 12
    • 33646294740 scopus 로고    scopus 로고
    • 5 and a minor component (2-5%) that does not and is hence ignored
    • 5 and a minor component (2-5%) that does not and is hence ignored.
  • 13
    • 33646282071 scopus 로고    scopus 로고
    • The transient absorption spectrum at times longer than the triplet decay has a feature around 670 nm assigned lo the radical cation of ZnDMb/ZnD(dme)Mh, along with one at 562 nm attributed to reduced cyt b$
    • ( 13) The transient absorption spectrum at times longer than the triplet decay has a feature around 670 nm assigned lo the radical cation of ZnDMb/ZnD(dme)Mh, along with one at 562 nm attributed to reduced cyt b$.
  • 14
    • 33646282922 scopus 로고    scopus 로고
    • 3+P Forster energy transfer depends on the emission spectrum of the energy donor, which is the same for ZnDMb and ZnD(dme)Mb (Turro, N. J. Modem Molecular Photochemistry; Benjamin/ Cummings Publishing Co.: Menlo Park, CA, 1978). Consequently any increase in energy transfer with esterificalion must come from a charge in the distribution of binding geometries, as discussed below
    • 3+P Forster energy transfer depends on the emission spectrum of the energy donor, which is the same for ZnDMb and ZnD(dme)Mb (Turro, N. J. Modem Molecular Photochemistry; Benjamin/ Cummings Publishing Co.: Menlo Park, CA, 1978). Consequently any increase in energy transfer with esterificalion must come from a charge in the distribution of binding geometries, as discussed below.
  • 16
    • 33646273003 scopus 로고    scopus 로고
    • Binding constants were measured on a MicroCal isothermal titration calorimeter; experimental conditions were the same as in the photolysis experiments except the protein concentration is in the mM range
    • Binding constants were measured on a MicroCal isothermal titration calorimeter; experimental conditions were the same as in the photolysis experiments except the protein concentration is in the mM range.
  • 21
    • 0345330736 scopus 로고
    • 5 and intraprotein ET reaction of rutheniummodified ZnMb (Winkler. J. R.; Gray, H. B. Chem. Re,. 1992. 92. 369-379.
    • (1992) Chem. Re,. , vol.92 , pp. 369-379
    • Winkler, J.R.1    Gray, H.B.2
  • 24
    • 33646285224 scopus 로고    scopus 로고
    • note
    • This is consistent with the observation that neutralisation has no effect on the steady-state fluorescence, energy-transfer quenching titration, which should be dominated by the majority conformations. Time-resolved fluorescence quenching studies will be performed to give further insight into this issue.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.