Identification of putative active-site residues in the DNase domain of colicin E9 by random mutagenesis

Journal of Molecular Biology

Volumn 260, Issue 5, 1996, Pages 731-742

  Garinot Schneider, Carole ^a   Pommer, Ansgar J ^a   Moore, Geoffrey R ^a   Kleanthous, Colin ^a   James, Richard ^a  

^a UNIVERSITY OF EAST ANGLIA   (United Kingdom)

Author keywords

DNase activity; E colicins; Gel shift assay; His tag fusions; Protein purification

Indexed keywords

BACTERIAL TOXIN; COLICIN; DEOXYRIBONUCLEASE; DNA BINDING PROTEIN; ENDONUCLEASE; MUTANT PROTEIN; PYOCIN; TRANSCRIPTION FACTOR;

AMINO ACID SUBSTITUTION; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA SEQUENCE; DRUG CYTOTOXICITY; ENZYME ACTIVE SITE; GENE MUTATION; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RESTRICTION MAPPING; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

BACTERIA (MICROORGANISMS); EUKARYOTA;

EID: 0030564827     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0433     Document Type: Article

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