Coumarin-fluorescein pair as a new donor-acceptor set for fluorescence energy transfer study of DNA

Tetrahedron Letters

Volumn 41, Issue 15, 2000, Pages 2605-2608

  Mitsui, Tsuneo ^a   Nakano, Hidehiko ^a   Yamana, Kazushige ^a  

^a UNIVERSITY OF HYOGO   (Japan)

Author keywords

Coumarin fluorescein pair; DNA; Fluorescence energy transfer; Nucleoside

Indexed keywords

COUMARIN; FLUORESCEIN;

ARTICLE; DNA DETERMINATION; DNA STRUCTURE; ENERGY TRANSFER; FLUORESCENCE;

EID: 0034620939     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(00)00215-X     Document Type: Article

References (17)

1. (a) Murchie, A. I. H.; Clegg, R. M.; Kitzing, E.; Duckett, D. R.; Diekmann, S.; Lilley, D. M. J. Nature 1989, 341, 763.
2. (b) Clegg, R. M.; Murchie, A. I. H.; Zechel, A.; Carlberg, C.; Diekmann, S.; Lilley, D. M. J. Biochemistry 1992, 31, 4846.
3. (c) Eis, P. S.; Millar, D. P. Biochemistry 1993, 32, 13852.
4. (a) Gohlke, C.; Murchie, A. I. H.; Lilley, D. M. J.; Clegg, R. M. Proc. Natl. Acad. Sci. USA 1994, 91, 11660.
5. (b) Ozaki, H.; Iwase, N.; Sawai, H.; Kodama, T.; Kyogoku, Y. Biochem. Biophy. Res. Comm. 1997, 231, 553.
6. Tuschl, T.; Gohlke, C.; Jovin, T. M.; Westhof, E.; Eckstein, F. Science 1994, 266, 785.
7. Yamana, K.; Mitsui, T.; Hayashi, H.; Nakano, H. Tetrahedron Lett. 1997, 38, 5815.
8. Yamana, K.; Mitsui, T.; Nakano, H. Tetrahedron 1999, 55, 5815.
9. 2 is the orientation factor, J is the spectral overlap integral, n is the refractive index of the medium, and F is the fluorescence quantum yield of the donor in the absence of the acceptor.
10. Zolkarnik, G.; Negulescu, P. A.; Knapp, T. E.; Mere, L.; Burres, N.; Feng, L.; Whitney, M.; Roemer, K.; Tsien, R. Y. Science 1998, 279, 84.
11. (a) Woods, L. L.; Sopp, J. J. Org. Chem. 1965, 30, 312.
12. (b) Woods, L.L.; Johnson, D. J. Org. Chem. 1965, 30, 4343.
13. 2O exchange).
14. Ion-spray mass data for oligomers 3-5 were 3849.5 (calcd 3848.6), 3848.9 (calcd 3848.6), and 3849.0 (calcd 3848.6), respectively.
15. Yamana, K.; Ohashi, Y.; Nunota, K.; Nakano, H. Tetrahedron 1997, 53, 4265.
16. The fluorescein labeled oligonucleotides were synthesized by the published procedure (Ref. 5).
17. The distances between coumarin and fluorescein fluorophores estimated from the theory (Ref. 6) were in fairly good agreement with the values based on the molecular model of DNA.