fw2.2: A quantitative trait locus key to the evolution of tomato fruit size

Science

Volumn 289, Issue 5476, 2000, Pages 85-88

  Frary, Anne ^a   Nesbitt, T Clint ^a   Frary, Amy ^a,c   Grandillo, Silvana ^a,d   Van Der Knaap, Esther ^a   Cong, Bin ^a   Liu, Jiping ^a   Meller, Jaroslaw ^b   Elber, Ron ^b   Alpert, Kevin B ^a   Tanksley, Steven D ^a  

^a Department of Population Medicine and Diagnostic Sciences   (United States)

^b Mount Holyoke College ^*  (United States)

^c MOUNT HOLYOKE COLLEGE   (United States)

^d UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELE; ARTICLE; EVOLUTION; FRUIT; GENE LOCUS; GENE SEQUENCE; NONHUMAN; ONCOGENE C H RAS; PRIORITY JOURNAL; QUANTITATIVE TRAIT; TOMATO;

ALLELES; AMINO ACID SEQUENCE; CELL COUNT; CELL DIVISION; CLONING, MOLECULAR; CONTIG MAPPING; EVOLUTION; FRUIT; GENES, PLANT; GENETIC COMPLEMENTATION TEST; HUMANS; LYCOPERSICON ESCULENTUM; MOLECULAR SEQUENCE DATA; MUTATION; ONCOGENE PROTEIN P21(RAS); PLANT COMPONENTS; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; PROTEIN STRUCTURE, SECONDARY; QUANTITATIVE TRAIT, HERITABLE; SEQUENCE ALIGNMENT; TRANSFORMATION, GENETIC;

LYCOPERSICON ESCULENTUM;

EID: 0034617236     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.289.5476.85     Document Type: Article

References (25)

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10. The Expand Long Template PCR System (Boehringer Mannheim) was used.
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13. The presence of the transgene was assayed by PCR and Southern hybridization analyses.
14. A total of 11 primary transformants were generated for cos50. Although all of these plants carried nptll, only two individuals (fw71 and fw107) contained the L. pennellii portion of the transferred DNA, as determined by PCR analysis with primers designed from the L. pennellii sequence of cos50.
15. Cosmid sequencing is described at Science Online at www.sciencemag.org/feature/data/1050401.shl.
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18. The predicted ORFX protein was compared to a training set of 594 structures (chosen from the Protein Data Base to eliminate redundancy) by using the LOOPP algorithm (J. Meller and R. Elber, in preparation). See also www.tc.cornell.edu/reports/NIH/resource/ CompBiologyTools/loopp/.
19. The three-dimensional structure of c-H-ras p21 (6q21) is shown at Science Online at www. sciencemag.org/feature/data/1050401.shl.
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23. Seed number is included in the analysis because reduced fertility, as evidenced by reduced seed per fruit, can decrease fruit size. Thus, these data show that the change in fruit size associated with cos50 is not a byproduct of reduced fertility.
24. The alignment of LpORFX and LeORFX with a total of 26 genes is shown at Science Online at www. sciencemag.org/feature/data/1050401.shl.
25. We thank J. Nasrallah, C. Aquadro, J. Doebley, K. Schmid, and W. Swanson for critical review of the manuscript. We also thank C. Lewis and N. van Eck for technical assistance. Supported by grants to S.D.T. from the National Research Initiative Cooperative Grants Program, U.S. Department of Agriculture Plant Genome Program (No. 97-35300-4384); the National Science Foundation (No. DBI-9872617); and the Binational Agricultural Research and Development Fund (No. US 2427-94) and by a grant from the NIH NCRR (National Center for Research Resources) to R.E. for development of LOOPP at Cornell Theory Center. We dedicate this paper to the memory of Dr. Kevin Alpert whose research inspired this work.