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Volumn 10, Issue 3, 2000, Pages 249-251

Recognition and inhibition of HIV integrase by a novel dinucleotide

Author keywords

Antivirals; Enzyme inhibitors; Nucleotides

Indexed keywords

CELL DNA; DINUCLEOTIDE; INTEGRASE INHIBITOR; ISODEOXYADENOSINE; PHOSPHATE; UNCLASSIFIED DRUG; VIRUS DNA;

EID: 0034615051     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(99)00677-0     Document Type: Article
Times cited : (19)

References (17)
  • 15
    • 0003555898 scopus 로고    scopus 로고
    • 32P-labeled linear oligonucleotide substrate (20 nM) was added, and incubation was continued for 1 h. Reactions were quenched by the addition of an equal volume (16 μL) of loading dye (98% deionized formamide, 10 mM EDTA, 0.025% xylene cyanol, 0.025% bromophenol blue). An aliquot (5 μL) was electrophoresed on a denaturing 20% polyacrylamide gel (0.09 M Tris-borate (pH 8.3), 2 mM EDTA, 20% acrylamide, 8 M urea). Gels were dried, exposed in a PhosphorImager cassette, and analyzed with a Molecular
    • 50) were determined by plotting the log of drug concentration versus percent inhibition and identifying the concentration which produced an inhibition of 50%.
    • (1998) In Methods in Cellular and Molecular Biology: Antiviral Evaluation
    • Mazumder, A.1    Neamati, N.2    Sundar, S.3    Owen, J.4    Pommier, Y.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.