Engineering direct fructose production in processed potato tubers by expressing a bifunctional alpha-amylase/glucose isomerase gene complex

Biotechnology and Bioengineering

Volumn 70, Issue 1, 2000, Pages 9-16

  Suvajittanont, W ^a   McGuire, J ^a   Bothwell, M K ^a  

^a Oregon State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; BIODEGRADATION; BIOSYNTHESIS; ENZYMES; FRUCTOSE; GENETIC ENGINEERING; GLUCOSE; HIGH TEMPERATURE EFFECTS; PLANTS (BOTANY);

AMYLASE; GENE ENCODING; GLUCOSE ISOMERASE;

STARCH;

AMYLASE; FRUCTOSE; GENE PRODUCT; GLUCOSE ISOMERASE; MICROBIAL ENZYME; STARCH; STARCH SYNTHASE;

ARTICLE; CARBOHYDRATE SYNTHESIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STABILITY; FRUCTOSE METABOLISM; FUSION GENE; GENETIC ENGINEERING; GEOBACILLUS STEAROTHERMOPHILUS; GLUCOSE METABOLISM; NONHUMAN; PHYTOCHEMISTRY; POTATO; PROTEIN EXPRESSION; TEMPERATURE DEPENDENCE; THERMAL EXPOSURE; THERMOSTABILITY; THERMUS THERMOPHILUS; TRANSGENIC PLANT;

ALDOSE-KETOSE ISOMERASES; ALPHA-AMYLASE; AMINO ACID SEQUENCE; BACILLUS STEAROTHERMOPHILUS; BIOTECHNOLOGY; BLOTTING, SOUTHERN; CLONING, MOLECULAR; DNA, PLANT; FRUCTOSE; GLUCOSE; MOLECULAR SEQUENCE DATA; PLANTS, GENETICALLY MODIFIED; PLASMIDS; POTATOES; PROMOTER REGIONS (GENETICS); SUPPORT, NON-U.S. GOV'T; TEMPERATURE; THERMUS THERMOPHILUS;

EID: 0034610055     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20001005)70:1<9::aid-bit2>3.0.co;2-7     Document Type: Article

References (47)

1. Agrobacterium-mediated transformation of three economically important potato cultivars using sliced internodal explants: An efficient protocol of transformation
2. Effect of ploidy and homozygosity on transgene expression in primary tobacco transformants and their androgenetic progenies
3. Biosynthesis and degradation of starch in higher plants
4. A rapid and sensitive method for the quantitation of microgram quantifies of protein utilizing the principle of protein dye binding
5. Multienzyme systems obtained by gene fusion
6. The sugar balance in some british potato varieties during storage. II. The effects of tuber age, previous storage temperature, and intermittent refrigeration upon low-temperature sweetening
7. Glucose isomerase
8. Enzymes involved in the processing of starch to sugars
9. Xylose (glucose) isomerase from the thermophile Thermus thermophilus: Cloning, sequencing, and comparison with other thermostable xylose isomerases
10. Introduction of sense and antisense cDNA for branching enzyme in the amylose-free potato mutant leads to physico-chemical changes in the starch
11. Molecular analysis of carbon partitioning in solanaceous species
12. Regulation of sucrose and starch metabolism in potato tubers in response to short-term water deficit
13. Overexpression of pyrophosphatase leads to increased sucrose degradation and starch synthesis, increased activities of enzymes for sucrose-starch interconversions, and increased levels of nucleotides in growing potato tubers
14. Antisense repression of both ADP-glucose pyrophosphorylase and triose phosphate translocator modifies carbohydrate partitioning in potato leaves
15. Enzymatic starch utilization and genetic engineering
16. Field evaluation of antisense RNA mediated inhibition of GBSS gene expression in potato
17. Factors affecting the inhibition by antisense RNA of granule-bound starch synthase gene expression in potato
18. Enhanced fructose levels in field grown potato tubers expressing a thermostable glucose isomerase
19. The design of a simple competitive Elisa using human proinsulin-alkalin phosphatase conjugates prepared by gene fusion
20. Construction of an artificial bifunctional enzyme, β-galactosidase/galactose dehydrogenase, exhibiting efficient galactose channeling
21. Starch biosynthesis
22. Inhibition of ADP-glucose pyrophosphorylase leads to sugar storing tubers and influences tuber formation and expression of tuber storage protein genes
23. Approaches to influences starch quantity and starch quality in transgenic plants
24. A revised medium for rapid growth and bioassays with tobacco tissue cultures
25. Nucleotide sequence of the Bacillus stearothermophilus α-amylase gene
26. Hybrid enzymes: Manipulating enzyme design
27. Direct gene transfer to plants
28. Production of active Bacillus licheniformis alpha-amylase in tobacco and its application in starch liquefaction
29. Microbial fructan production in transgenic potato plants and tubers
30. Synthesis of fructans in tubers of transgenic starch-deficient potato plants does not result in an increased allocation of carbohydrates
31. Mutation: Modified starch metabolism in mutant and transgenic plants
32. Production and use of enzymes degrading starch and some other polysaccharides
33. Modifying starch biosynthesis with transgenes in potatoes
34. Subcellular manipulation of sucrose metabolism in transgenic plants
35. Manipulation of sink-source relations in transgenic plants
36. Regulation of metabolism in transgenic plants
37. Combined expression of glucokinase and invertase in potato tubers leads to a dramatic reduction, in starch accumulation and a stimulation of glycolysis
38. Fructan as a new carbohydrate sink in transgenic potato plants
39. Potato granule-bound starch synthase promoter-controlled GUS expression: Regulation of expression after transient and stable transformation
40. Cristallinity in starch plastics: Consequences for material properties
41. Expression of a chimeric granule-bound starch synthase-GUS gene in transgenic potato plants
42. Inhibition of the expression of the gene for granule-bound starch synthase in starch by antisense constructs
43. Towards modifying plants for altered starch content and composition
44. Complete nucleotide sequence of a gene coding for heat- and pH-stable α-amylase of Bacillus licheniformis: Comparison of the amino acid sequences of Three bacterial liquefying α-amylases deduced from the cDNA sequences
45. Evidence of the crucial role of sucrose synthase for sink strength using transgenic potato plants (Solanum tuberosum L.)
46. Soluble acid invertase determines the hexose-to-sucrose ratio in cold-stored potato tubers