The H159A mutant of yeast enolase 1 has significant activity

Biochemical and Biophysical Research Communications

Volumn 276, Issue 3, 2000, Pages 1199-1202

  Brewer, John M ^a   Holland, Michael J ^b   Lebioda, Lukasz ^c  

^a UNIVERSITY OF GEORGIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

Cloning; Enolase; Expression; Folding; Mechanism; Mutagenesis

Indexed keywords

ENOLASE; HISTIDINE;

ARTICLE; ENZYME ACTIVITY; ENZYME MECHANISM; ESCHERICHIA COLI; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; YEAST;

EID: 0034610022     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.3618     Document Type: Article

References (25)

1. Enolase
2. Yeast enolase: Mechanism of activation by metal ions
3. Calorimetric studies of the role of magnesium ions in yeast enolase catalysis
4. Magnesium ion requirements for enolase activity
5. 2+-2-phosphoglycerate/phosphoenolpyruvate complex at 2.2Å resolution
6. 2+-phosphono-acetohydroxamate complex at 2.4Å resolution
7. A carboxylate oxygen of the substrate bridges the magnesium ions at the active site of enolase: Structure of the yeast enzyme complexed with the equilibrium mixture of 2-phosphoglycerate and phosphoenolpyruvate at 1.8Å resolution
8. Mechanism of enolase: The crystal structure of asymmetric dimer enolase-2-phospho-D-glycerate/enolasephosphoenolpyruvate at 2.0Å resolution
9. X-ray structure and catalytic mechanism of lobster enolase
10. 31P-NMR studies of the effect of various metals on substrate binding to yeast enolase
11. The role of His159 in yeast enolase catalysis
12. Enzymatic function of loop Movement in enolase: H159N/S307Y, H159A, H159F and N207A enolases
13. Targeted deletion of a yeast enolase structural gene-identification and isolation of yeast enolase isozymes
14. Preparation and characterization of the E168Q site-directed mutant of yeast enolase 1
15. Preparation by site-directed mutagenesis and characterization of the E211Q mutant of yeast enolase 1
16. Significance of the enzymatic properties of yeast S39A enolase to the catalytic mechanism
17. 2+ activation of and binding to yeast enolase: A functional study
18. Studies on two high-affinity enolase inhibitors, chemical characterization
19. Reaction intermediate analogues for enolase
20. Enolase from yeast and rabbit muscle
21. Effect of site-directed mutagenesis of His373 of yeast enolase on some of its physical and enzymatic properties
22. Studies on the enzyme enolase: II, Kinetic studies
23. Studies on two high-affinity enolase inhibitors, reaction with enolases
24. Kinetic and magnetic resonance studies of active-site mutants of staphylococcal nuclease: Factors contributing to catalysis
25. Toward identification of acid base catalysts in the active site of enolase: Comparison of the properties of K345A, E168Q and E211Q variants