Toward identification of acid/base catalysts in the active site of enolase: Comparison of the properties of K345A, E168Q, and E211Q variants

Biochemistry

Volumn 35, Issue 5, 1996, Pages 1692-1699

  Poyner, Russell R ^a   Laughlin, L Timothy ^a,b   Sowa, Gwendolyn A ^a   Reed, George H ^a  

^a UNIVERSITY OF WISCONSIN   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENOLASE; GENE PRODUCT; GLUTAMIC ACID; LYSINE; MUTANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SUBSTRATE; ESCHERICHIA COLI; GLYCOLYSIS; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

BASE SEQUENCE; BINDING SITES; CATALYSIS; GLYCERIC ACIDS; HYDROLYSIS; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHOENOLPYRUVATE; PHOSPHOPYRUVATE HYDRATASE; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TARTRONATES; YEASTS;

ESCHERICHIA COLI; PHRAGMITES;

EID: 0030019648     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi952186y     Document Type: Article

References (36)

1. Anderson, S. R., Anderson, V. E., & Knowles, J. R. (1994) Biochemistry 33, 10545-1055.
2. Anderson, V. E., Weiss, P. M., & Cleland, W. W. (1984) Biochemistry 29, 10498-10503.
3. Ash, D. E., Goodhart, P. J., & Reed, G. H. (1984) Arch. Biochem. Biophys. 228, 31-40.
4. Bowman, E. D., McQueney, M. S., Scholten, J. D., & Dunaway-Mariano, D. (1990) Biochemistry 29, 7059-7063.
5. Brewer, J. M., Robson, R., Glover, C. V. C., Holland, M. J., & Lebioda, L. (1993) Proteins: Struct., Funct., Genet. 17, 426-434.
6. Burbaum, J. J., & Knowles, J. R. (1989) Biochemistry 28, 9306-9317.
7. Cleland, W. W. (1979) Methods Enzymol. 63, 103-138.
8. Cleland, W. W. (1992) Biochemistry 31, 317-319.
9. Cohn, M., Pearson, J. E., O'Connell, E. L., & Rose, I. A. (1970) J. Am. Chem. Soc. 92, 4095-4098.
10. Duquerroy, S., Camus, C., & Janin, J. (1995) Biochemistry 34, 12513-12523.
11. Dinovo, E. C., & Boyer, P. D. (1971) J. Biol. Chem. 246, 4586-4593.
12. Gerlt, J. A., & Gassman, P. G. (1992) J. Am. Chem. Soc. 114, 5928-5934.
13. Gerlt, J. A., & Gassman, P. G. (1993) J. Am. Chem. Soc. 115, 11552-11568.
14. Harpel, M. R., & Hartman, F. C. (1994) Biochemistry 33, 5553-5561.
15. Holland, M. J., Holland, J. P., Thill, G. P., & Jackson, K. A. (1981) J. Biol. Chem. 256, 1385-1395.
16. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950.
17. Landro, J. A., Kallarakal, A., Ransom, S. C., Gerlt, J. A., Kozarich, J. W., Neidhart, D. J., & Kenyon, G. L. (1991) Biochemistry 30, 9274-9281.
18. Latwesen, D. G., Poe, M., Leigh, J. S., & Reed, G. H. (1992) Biochemistry 31, 4946-4950.
19. Lebioda, L., & Stec, B. (1991) Biochemistry 30, 2817-2822.
20. Liu, J., Peliska, J. A., & O'Leary, M. H. (1990) Arch. Biochem. Biophys. 277, 143-148.
21. Michael, S. F. (1994) BioTechniques 16, 410-412.
22. Nowak, T., Mildvan, A. S., & Kenyon, G. L. (1973) Biochemistry 12, 1690-1701.
23. Poyner, R. R., & Reed, G. H. (1992) Biochemistry 31, 7166-7173.
24. Poyner, R. R. (1993). Ph.D. Dissertation, University of Wisconsin-Madison.
25. Sangadala, V. S., Glover, C. V. C., Robson, R. L., Holland, M. J., Lebioda, L., & Brewer, J. M. (1995) Biochim. Biophys. Acta 1251, 23-31.
26. Sekimoto, T., Matsuyama, T., Fukui, T., & Tanizawa, K. (1993) J. Biol. Chem. 268, 27039-27045.
27. Spring, T. G., & Wold, F. (1971a) J. Biol. Chem. 246, 6797-6802.
28. Sprine, T. G., & Wold, F. (1971b) Biochemistry 10, 4655-4660.
29. Stubbe, J. A., & Abeles, R. H. (1980) Biochemistry 19, 5505-5512.
30. Stubbe, J. A., & Kenyon, G. L. (1972) Biochemistry 11, 338-345.
31. Toney, M. D., & Kirsch, J. F. (1989) Science 243, 1485-1488.
32. Wedekind, J. E., Poyner, R. R., Reed, G. H., & Rayment, I. (1994) Biochemistry 33, 9333-9342.
33. Wedekind, J. E., Reed, G. H., & Rayment, I. (1995) Biochemistry 34, 4325-4330.
34. Weiss, P. M., Boerner, R. J., & Cleland, W. W. (1987) J. Amer. Chem. Soc. 109, 7201-7202.
35. Weiss, P. M., Boerner, R. J., & Cleland, W. W. (1989) Biochemistry 28, 1634-1641.
36. Zhukovsky, E. A., Robinson, P. R., & Oprian, D. D. (1991) Science 252, 558-560.