Ribosomal protein L2 is involved in the association of the ribosomal subunits, tRNA binding to A and P sites and peptidyl transfer

EMBO Journal

Volumn 19, Issue 19, 2000, Pages 5241-5250

  Diedrich, Gundo ^a,b   Spahn, Christian M T ^a,c   Stelzl, Ulrich ^a   Schäfer, Markus A ^a   Wooten, Tammy ^d   Bochkariov, Dmitry E ^e   Cooperman, Barry S ^d   Traut, Robert R ^e   Nierhaus, Knud H ^a  

^a MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^d UNIVERSITY OF PENNSYLVANIA   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Peptidyl transfer; Ribosomal protein L2; Ribosome; Translation; tRNA

Indexed keywords

RIBOSOME PROTEIN;

ARTICLE; BINDING SITE; ESCHERICHIA COLI; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; RIBOSOME SUBUNIT; RNA BINDING; RNA TRANSLATION; STRUCTURE ANALYSIS;

EID: 0034596998     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/19.19.5241     Document Type: Article

References (48)

1. Placement of protein and RNA structures into a 5 Å-resolution map of the 50S ribosomal subunit
2. Mutations in ribosomal proteins L7/L12 perturb EF-G and EF-Tu functions
3. Ribosomes and polysomes
4. Substrate and antibiotic binding sites at the peptidyl transferase centre of E.coli ribosomes: Binding of UACCA-leu to 50S subunits
5. Cooperative and antagonistic interactions of peptidyl-tRNA and antibiotics with bacterial ribosomes
6. Antibiotic probes of E.coli ribosomal peptidyl transferase
7. Histidine 229 in the protein L2 is apparently essential for 50S peptidyl transferase activity
8. Contact patterns of tRNAs do not change during ribosomal translocation
9. Large-scale isolation of proteins of the large subunit from Escherichia coil ribosomes
10. Total reconstitution and assembly of 50S subunits from E.coli ribosomes in vitro
11. Ribosome-catalyzed ester formation
12. Ribosomal proteins L15 and L16 are mere late assembly proteins of the large ribosomal subunit
13. A model of the translational apparatus based on a three-dimensional reconstruction of the Escherichia coli ribosome
14. An improved method for 2-d gel electrophoresis: Analysis of mutationally altered ribosomal proteins of E.coli
15. Reconstitution of the 50S subunit with in vitro-transcribed 23S rRNA: A new tool for studying peptidlytransferase
16. Characterization of functionally active subribosomal particles from Thermus aquaticus
17. Peptidyl transferase activity catalyzed by protein-free 23S ribosomal RNA remains elusive
18. Base-pairing between 23S rRNA and tRNA in the ribosomal A site
19. The subunit interface of the E.coli ribosome. Identification of proteins at the interface between the 30S and 50S subunits by crosslinking with 2-iminothiolane
20. Three-dimensional reconstruction of the Escherichia coli 30S ribosomal subunit in ice
21. Ribosome-catalysed peptidyl transfer: The polyphenylalanine system
22. Catalysis of peptide bond formation by 50S ribosomal subunits from Escherichia coli
23. Phylogenetic relationship of organisms by ribosomal protein comparison
24. Reconstitution of ribosomes
25. Molecular mechanisms of the ribosomal peptidyltransferase center
26. The elongating ribosome: Structural and functional aspects
27. Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNA domains
28. Possible involvement of Escherichia coli 23S ribosomal RNA in peptide bond formation
29. Peptide bond formation: Retraction
30. Unusual resistance of peptidyltransferase to protein extraction procedures
31. Preparation and activity measurements of deuterated 50S subunits for neutron-scattering analysis
32. Functional analysis of ribosomal protein L2 in yeast mitochondria
33. Studies on the formation of transfer ribonucleic acid-ribosome complexes. X. Phenylalanyl-oligonucleotide binding to ribosomes and the mechanism of chloramphenicol action
34. The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae
35. The third ribosomal tRNA-binding site, the E site, is occupied in native polysomes
36. Identification of a region of Escherichia coli ribosomal protein L2 required for the assembly of L16 into the 50S ribosomal subunit
37. Peptidyl transferase - Involvement of histidine in substrate binding and peptide bond formation
38. Kinetic and thermodynamic parameters for transfer RNA binding to the ribosome and for the translocation reaction
39. Minimal set of ribosomal components for the reconstitution of the peptidyltransferase activity
40. Mapping of Escherichia coli ribosomal components involved in peptidyl tranferase activity
41. Mutational analysis of two highly conserved UGG sequences of 23S rRNA from Escherichia coli
42. Conserved nucleotides of 23S rRNA located at the ribosomal peptidyltransferase center
43. Functional implications of ribosomal protein L2 in protein biosynthesis as shown by in vivo replacement studies
44. Cooperative binding of 3' fragments of tRNA to the peptidyltransferase centre of E.coli ribosomes
45. Identification of 50S components neighboring 23S rRNA nucleotides A2448 and U2604 within the peptidyl transferase center of Escherichia coli ribosomes
46. The photochemical inactivation of peptidyl transferase activity
47. A model of the tRNA binding sites on the E.coli ribosome
48. Peptidyl-transferase ribozymes: Trans reactions, structural characterization and ribosomal RNA-like features