Site-Directed Mutagenesis of Cytochrome P450scc (CYP11A1). Effect of Lysine Residue Substitution on Its Structural and Functional Properties

Biochemistry (Moscow)

Volumn 65, Issue 12, 2000, Pages 1409-1418

  Lepesheva, G I ^a   Azeva, T N ^a   Strushkevich, N V ^a   Gilep, A A ^b   Usanov, S A ^b  

^a INSTITUTE OF BIOORGANIC CHEMISTRY   (Belarus)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Cytochrome P450scc; Heterologous expression; Protein protein interactions; Site directed mutagenesis

Indexed keywords

ADRENODOXIN; CHOLESTEROL MONOOXYGENASE (SIDE CHAIN CLEAVING); GLUTAMIC ACID; HEME; LYSINE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; ESCHERICHIA COLI; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN FOLDING; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; SPECTROPHOTOMETRY; THERMODYNAMICS; TIME;

ADRENODOXIN; AMINO ACID SEQUENCE; ANIMALS; CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GLUTAMIC ACID; HEME; HUMANS; LYSINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN FOLDING; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROPHOTOMETRY; THERMODYNAMICS; TIME FACTORS;

EID: 0034576247     PISSN: 00062979     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1002809024292     Document Type: Article

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