Purification of GimC from Saccharomyces cerevisiae

Methods in Molecular Biology

Volumn 140, Issue , 2000, Pages 185-193

  Siegers, Katja ^a   Schiebel, Elmar ^a  

^a BEATSON INSTITUTE FOR CANCER RESEARCH   (United Kingdom)

Author keywords

Generate Polymerase Chain Reaction Product; Lithium Acetate; Simple Polymerase Chain Reaction; Slight Agitation; Yeast Cell

Indexed keywords

CHAPERONE; FUNGAL PROTEIN; HYBRID PROTEIN; PREFOLDIN; PRIMER DNA;

ARTICLE; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

BASE SEQUENCE; DNA PRIMERS; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE;

MLCS; MLOWN;

EID: 0034575340     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1385/1-59259-061-6:185     Document Type: Chapter

References (13)

1. Geissler, S., Siegers, K., and Schiebel, E. (1998) A novel protein complex promoting formation of functional and tubulin. EMBO J. 17, 952–966.
2. Siegers, K., Waldmann, T., Leroux, M. R., Grein, K., Shevchenko, A., Schiebel, E., et al. (1999) Compartmentation of protein folding in vivo: Sequestration of non-native polypeptide by the chaperonin-GimC system. EMBO J. 18, in press.
3. Wach, A., Brachat, A., Pohlmann, R., and Philippsen, P. (1994) New heterolo-gous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793–1808.
4. Longtine, M. S., McKenzie, A., Demarini, D. J., Shah, N. G., Wach, A., Brachat, A., et al. (1998) Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14, 953–961.
5. Knop, M., Siegers, K., Pereira, G., Zachariae, W., Winsor, B., Nasmyth, K., et al. (1999) Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines. Yeast 15, 963–972.
6. Drubin, D. G. (1990) Actin and actin-binding proteins in yeast. Cell Motil. Cytoskel. 25, 7–11.
7. Neff, N. F., Thomas, J. H., Grisafi, P., and Botstein, D. (1983) Isolation of the ß-tubulin gene from yeast and demonstration of its essential function in vivo. Cell 33, 211–219.
8. Huffaker, T. C., Thomas, J. H., and Botstein, D. (1988) Diverse effects of b-tubulin mutations on microtubule formation and function. J. Cell Biol. 106, 1997–2010.
9. Sikorski, R. S. and Hieter, P. (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19–27.
10. Schiestl, R. H. and Gietz, R. D. (1989) High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr. Genet. 16, 339–346.
11. Wright, A. P. H., Bruns, M., and Hartley, B. S. (1989) Extraction and rapid inac-tivation of proteins from Saccharomyces cerevisiae by trichloroacetic acid precipitation. Yeast 5, 51–53.
12. Horvath, A. and Riezman, H. (1994) Rapid protein extraction from Saccharomyces cerevisiae. Yeast 10, 1305–1310.
13. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.