Expression and purification of antigenically active soluble derivatives of the heterodimeric and homodimeric forms of the mouse CD8 lymphocyte membrane glycoprotein

Journal of Immunological Methods

Volumn 246, Issue 1-2, 2000, Pages 149-163

  Pellicci, Daniel G ^a   Kortt, Alexander A ^b   Sparrow, Lindsay G ^b   Hudson, Peter J ^b   Sorensen, Henrik V ^c   Davis, Simon J ^c   Classon, Brendan J ^a  

^a MONASH UNIVERSITY MEDICAL SCHOOL   (Australia)

^b CSIRO   (Australia)

^c UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Affinity chromatography; Heterodimer; Soluble CD8 glycoprotein; Stoichiometry; T cell co receptor

Indexed keywords

CD8 ANTIGEN; COMPLEMENTARY DNA; DIMER; GLYCOPROTEIN; LYMPHOCYTE ANTIGEN; MEMBRANE PROTEIN; PROTEIN SUBUNIT; RECOMBINANT PROTEIN; T LYMPHOCYTE RECEPTOR;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ANTIGEN EXPRESSION; ANTIGEN RECOGNITION; ARTICLE; CELL CLONE; CONTROLLED STUDY; FIBROBLAST; IMMUNOAFFINITY CHROMATOGRAPHY; LIGAND BINDING; MOUSE; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECOMBINANT DNA TECHNOLOGY; STOICHIOMETRY; T LYMPHOCYTE SUBPOPULATION;

ANIMALS; ANTIGENS, CD8; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CRICETINAE; DIMERIZATION; MICE; PROTEIN ISOFORMS; RATS; SOLUBILITY;

MAMMALIA;

EID: 0034565082     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00280-5     Document Type: Article

References (32)

1. The hinge region of the CD8 alpha chain: structure, antigenicity, and utility in expression of immunoglobulin superfamily domains
2. Therapeutic potential of monovalent monoclonal antibodies
3. High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification
4. Disruption of T lymphocyte positive and negative selection in mice lacking the CD8 beta chain
5. High level expression in Chinese hamster ovary cells of soluble forms of CD4 T lymphocyte glycoprotein including glycosylation variants
6. The human Lyt-3 molecule requires CD8 for cell surface expression
7. Reduced thymic maturation but normal effector function of CD8+ T cells in CD8 beta gene-targeted mice
8. Crystal structure of the complex between human CD8alpha(alpha) and HLA-A2
9. CD8 enhances formation of stable T-cell receptor/MHC class I molecule complexes [see comments] [published erratum appears in Nature 1997 (June 5) 387 (6633) 634]
10. Molecular linkage of the Ly-3 and Ly-2 genes. Requirement of Ly-2 for Ly-3 surface expression
11. Genetic correlation of a mouse light chain variable region marker with a thymocyte surface antigen
12. The cytoplasmic domain of CD8 beta regulates Lck kinase activation and CD8 T cell development
13. A role for the cytoplasmic tail of the beta chain of CD8 in thymic selection
14. Beta-chain broadens range of CD8 recognition for MHC class I molecule
15. Expression, purification, and functional analysis of murine ectodomain fragments of CD8αα and CD8αβ dimers
16. Structural basis of CD8 coreceptor function revealed by crystallographic analysis of a murine CD8αα ectodomain fragment in complex with H-2 Kb
17. The structure of the CD4 and CD8 genes
18. CD8 modulation of T-cell antigen receptor-ligand interactions on living cytotoxic T lymphocytes
19. Requirement for CD8 beta chain in positive selection of CD8-lineage T cells
20. Cell-cell adhesion mediated by CD8 and MHC class I molecules
21. Molecular biology and function of CD4 and CD8
22. CD8 beta increases CD8 coreceptor function and participation in TCR-ligand binding
23. The V beta repertoire of mouse gut homodimeric alpha CD8+ intraepithelial T cell receptor alpha/beta+lymphocytes reveals a major extrathymic pathway of T cell differentiation
24. Thymic and extrathymic origins of gut intraepithelial lymphocyte populations in mice
25. A binding site for the T-cell co-receptor CD8 on the alpha 3 domain of HLA-A2
26. Chinese hamster ovary cell mutants with multiple glycosylation defects for production of glycoproteins with minimal carbohydrate heterogeneity
27. Significance of Lyt phenotypes: Lyt2 antibodies block activities of T cells that recognize class 1 major histocompatibility complex antigens regardless of their function
28. The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells
29. Ly antigens associated with T cell recognition and effector function
30. The CD8β ectodomain contributes to the augmented coreceptor function of CD8αβ heterodimers relative to CD8αα homodimers
31. T cell receptor and coreceptor CD8αα bind peptide-MHC independently and with distinct kinetics
32. The CD8 coreceptor revisited: one chain good, two chains better