Comparing the Utility of β-glucuronidase and Green Fluorescent Protein for Detection of Weak Promoter Activity in Arabidopsis thaliana

Plant Molecular Biology Reporter

Volumn 18, Issue 4, 2000, Pages 319-330

  Mantis, Joanna ^a   Tague, Brian W ^a  

^a WAKE FOREST UNIVERSITY   (United States)

Author keywords

Arabidopsis thaliana; AtZFP1; Coexpression; Green fluorescent protein; glucuronidase

Indexed keywords

EID: 0034563073     PISSN: 07359640     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02825059     Document Type: Review

References (24)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG and Smith JA (1989) Current Protocols in Molecular Biology, Green Publishing Associates Wiley Interscience, New York.
2. Bechtold N and Pelletier G (1998) In planta Agrobacterium-mediated transformation of adult Arabidopsis plants by vacuum infiltration. In: Martinez-Zapater JM and Salina J (eds), Methods in Molecular Biology: Arabidopsis Protocols, pp 259-266, volume 82, Humana Press, Totowa, NJ.
3. Chalfie M, Tu Y, Euskirchen G, Ward WW and Prasher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
4. 2 zinc finger protein 1, is expressed downstream of photomorphogenic activation. Plant Mol Biol 42: 279-290.
5. Clough SJ and Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16: 735-743.
6. Crameri A, Whiteborn EA, Tate E and Stemmer WPC (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat Biotechnol 14: 315-319.
7. Cutler SR, Ehrhardt DW, Griffitts JS and Somerville CR (2000) Random GFP:cDNA fusions enable visualization of subcellular structures of Arabidopsis at a high frequency. Proc Natl Acad Sci USA 97: 3718-3723.
8. Gadella TWJ (1997) Fluorescence lifetime imaging microscopy. Eur Microscopy Anal 47: 9-11.
9. Haseloff J, Siemering KB, Prasher DC and Hodge S (1997) Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly. Proc Natl Acad Sci USA 94: 2122-2127.
10. Heim R, Cubitt AB and Tsein RY (1995) Improved green fluorescence. Nature 373: 663-663.
11. Heim R, Prasher DC and Tsien RY (1994) Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc Natl Acad Sci USA 91: 12501-12504.
12. Huang Z, Han Y and Howell SH (2000) Formation of surface tubules and fluorescent foci in Arabidopsis thaliana protoplasts expressing a fusion between the green fluorescent protein and the cauliflower mosaic virus movement protein. Virology 271: 58-64.
13. Jefferson RA, Kavanaugh TA and Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker for higher plants. EMBO J 6: 3901-3907.
14. Koorneef M, Alonso-Blanco C and Stam P (1998) Genetic Analysis. In: Martinez-Zapater JM and Salina J (eds), Arabidopsis Protocols, pp 105-117, Humana Press, Totowa, NJ.
15. Kozak M (1984) Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucl Acids Res 12: 857-873.
16. Logan DC and Leaver CJ (2000) Mitochondria-targeted GFP highlights the heterogeneity of mitochondrial shape, size, and movement within living plant cells. J Exp Bot 51: 865-871.
17. Mantis J (2000) The essential upstream sequence of the Arabidopsis thaliana zinc finger protein 1 gene, AtZFP1. Master's Thesis. Department of Biology, Wake Forest University, Winston-Salem NC.
18. Pesole G, Liuni S, Grillo G and Saccone C (1998) UTRdb: a specialized database of 5′ and 3′-untranslated regions of eukaryotic mRNAs. Nucl Acids Res 26: 192-195.
19. Quaedvlieg NEM, Schlaman HR, Admiraal PC, Wijting SE, Stougaard J and Spaink H (1998) Fusions between green fluorescent protein and β-glucuronidase as sensitive and vital bifunctional reporters in plants. Plant Mol Biol 38: 861-873.
20. Rigler R, Mets U, Widengren J and Kask P (1997) Fluorescence correlation spectroscopy with high count rate and low background. Eur Biophys J 22: 196-176.
21. Song JY, Leung T, Ehler LK, Wang CX and Liu ZC (2000) Regulation of meristem organization and cell division by TSO1, an Arabidopsis gene with cysteine-rich repeats. Development 127: 2207-2217.
22. 2 zinc finger protein gene. Plant Mol Biol 32: 785-796.
23. Tague BW and Goodman HM (1995) Characterization of a family of Arabidopsis zinc finger protein cDNAs. Plant Mol Biol 28: 267-279.
24. Taylor CB (1997) Promoter fusion analysis: an insufficient measure of gene expression. Plant Cell 9: 273-275.