A gene containment strategy based on a restriction-modification system

Environmental Microbiology

Volumn 2, Issue 5, 2000, Pages 555-563

  Torres, Begoña ^a   Jaenecke, Susanne ^b   Timmis, Kenneth N ^b   García, José Luis ^a   Díaz, Eduardo ^a  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BASE SEQUENCE; DEOXYRIBONUCLEASE ECORI; DNA MODIFICATION METHYLASES; DNA, BACTERIAL; ECOSYSTEM; ENVIRONMENTAL MICROBIOLOGY; GENE TRANSFER, HORIZONTAL; GENETIC MARKERS; MOLECULAR SEQUENCE DATA; MUTATION; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

BACTERIA (MICROORGANISMS);

EID: 0034551744     PISSN: 14622912     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1462-2920.2000.00138.x     Document Type: Article

References (45)

1. A conditional suicide system in Escherichia coli based on the intracellular degradation of DNA
2. Basic local alignment search tool
3. Short-sequence DNA repeats in prokaryotic genomes
4. Biology of DNA restriction
5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
6. A review of available systems to investigate transfer of DNA to indigenous soil bacteria
7. Millennium bugs
8. Insertion of native insertion sequence elements
9. Universal barrier to lateral spread of specific genes among microorganisms
10. Design of genetic circuits for restricting gene and biocatalyst dispersal
11. Properties of six pesticide degradation plasmids isolated from Alcaligenes paradoxus and Alcaligenes eutrophus
12. Horizontal gene transfer as a biosafety issue: A natural phenomenon of public concern
13. DNA sequence organization of the IS10-right of Tn10 and comparison with IS10-left
14. SOS induction as an in vivo assay of enzyme-DNA interactions
15. Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease
16. A stringently controlled expression system for analysing lateral gene transfer between bacteria
17. Purification, cloning and sequence analysis of Rsrl DNA methyltransferase: Lack of homology between two enzymes, Rsrl and EcoRI, that methylate the same nucleotide in identical recognition sequences
18. Recombinant DNA techniques for bioremediation and environmentally-friendly synthesis
19. Transposon Tn10
20. Development and testing of improved suicide functions for biological containment of bacteria
21. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes
22. Restriction-modification systems as genomic parasites in competition for specific sequences
23. Bacterial gene transfer by natural genetic transformation in the environment
24. Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5 and Tn10-derived mini-transposons
25. Insertion sequences
26. Gene transfer between distantly related bacteria
27. Gene cloning and manipulation in Pseudomonas
28. Conditional suicide system for containment of bacteria and plasmids
29. Suicidal genetic elements and their use in biological containment of bacteria
30. Expression of the EcoRI restriction-modification system and the construction of positive-selection cloning vectors
31. VTR expression cassettes for engineering conditional phenotypes in Pseudomonas: Activity of the Pu promoter of the TOL plasmid under limiting concentrations of the XyIR activator protein
32. Recognition and cleavage of DNA by type-II restriction endonucleases
33. Suicide microbes on the loose
34. Preparation of genomic DNA from bacteria. Miniprep of bacterial genomic DNA
35. Characterization of cell lysis in Pseudomonas putida induced upon expression of heterologous killing genes
36. DNA sequencing with chain-terminating inhibitors
37. IS5: A mobile enhancer of transcription in Escherichia coli
38. Four types of IS1 with differences in nucleotide sequence reside in the Escherichia coli K-12 chromosome
39. Environmental biotechnology
40. Release of recombinant microorganisms
41. R promoters by a cl repressor in a broad-host-range thermoregulated plasmid marker system
42. Gene transfer among bacteria in natural environments
43. Specificity of insertion of IS1