The ribosomal binding and peptidyl-tRNA hydrolysis functions of Escherichia coli release factor 2 are linked through residue 246

RNA

Volumn 6, Issue 12, 2000, Pages 1704-1713

  Wilson, D N ^a   Guevremont, D ^a   Tate, W P ^a  

^a UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

Peptidyltransferase; Release factors; RF2; Ribosome; Translational termination

Indexed keywords

AMINO ACID; CHIMERIC PROTEIN; PEPTIDE; PEPTIDYLTRANSFERASE; RECOMBINANT PROTEIN; RELEASE FACTOR 2; SERINE; THREONINE; TRANSFER RNA; UNCLASSIFIED DRUG;

ARTICLE; CODON; ENZYME ACTIVITY; ENZYME LOCALIZATION; ESCHERICHIA COLI; GENE CASSETTE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN RNA BINDING; RIBOSOME; SIGNAL TRANSDUCTION; TRANSLATION REGULATION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CODON; ESCHERICHIA COLI; HYDROLYSIS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PEPTIDE CHAIN TERMINATION, TRANSLATIONAL; PEPTIDE TERMINATION FACTORS; PHENOTYPE; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; RNA, TRANSFER, AMINO ACYL; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; THREONINE;

ESCHERICHIA COLI; PROKARYOTA;

EID: 0034548165     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S135583820000131X     Document Type: Article

References (30)

1. Mutations in RNAs of both ribosomal subunits cause defects in translation termination
2. Initiation factors of protein biosynthesis in bacteria and their structural relationship to elongation and termination factors
3. Two regions of the Escherichia coli 16S ribosomal RNA are important for decoding stop signals in polypeptide chain termination
4. Direct recognition of mRNA stop signals by Escherichia coli polypeptide chain release factor two
5. Characterization of three proteins involved in polypeptide chain termination
6. Peptide chain termination, codon, protein factor, and ribosomal requirements
7. Release factors: In vitro assay and purification
8. Indirect regulation of translations termination efficiency at highly expressed genes and recoding sites by the factor recycling function of Escherichia coli release factor RF3
9. Frameshift auto-regulation in the gene for Escherichia coli release factor 2: Partly functional mutants result in frameshift enhancement
10. Tightly controlled expression systems for the production and purification of Escherichia coli release factor 1
11. Mutations in the highly conserved GGQ motif of class 1 polypeptide release factors abolish ability of human eRF1 to trigger peptidyl-tRNA hydrolysis
12. A tripeptide 'anticodon' deciphers stop codons in messenger RNA
13. Efficient in vitro translational termination in Escherichia coli is constrained by the orientations of the release factor, stop signal and peptidyl-tRNA within the termination complex
14. Sequence and functional analysis of mutations in the gene encoding peptide-chain-release factor 2 of Escherichia coli
15. Specific protection of 16S rRNA by translational initiation factors
16. Functional domains in the Escherichia coli release factors. Activities of hybrids between RF-1 and RF-2
17. A single proteolytic cleavage in release factor 2 stabilizes ribosome binding and abolishes peptidyl-tRNA hydrolysis activity
18. How protein reads the stop codon and terminates translation
19. Genetic probes to bacterial release factors: tRNA mimicry hypothesis and beyond
20. Macromolecular mimicry
21. A direct estimation of the context effect on the efficiency of termination
22. Sequence comparison of new prokaryotic and mitochondrial members of the polypeptide chain release factor family predicts a 5-domain model for release factor structure
23. The identity of the base following the stop codon determines the efficiency of in vivo translational termination in Escherichia coli
24. The crystal structure of human eukaryotic release factor eRF1: Mechanism of stop codon recognition and peptidyl-tRNA hydrolysis
25. Termination of protein synthesis
26. Functional specificity of amino acid at position 246 in the tRNA mimicry domain of bacterial release factor 2
27. Factor-mediated termination of protein synthesis: A welcome return to the mainstream of translation