X-ray crystal structure and catalytic properties of Thr252Ile mutant of cytochrome P450cam: Roles of Thr252 and water in the active center

Journal of Biochemistry

Volumn 128, Issue 6, 2000, Pages 965-974

  Hishiki, Takako ^a   Shimada, Hideo ^a   Nagano, Shingo ^a   Egawa, Tsuyoshi ^a   Kanamori, Yasukazu ^a   Makino, Ryu ^a   Park, Sam Yong ^b   Adachi, Shin ichi ^b   Shiro, Yoshitsugu ^b   Ishimura, Yuzuru ^a  

^a KEIO UNIVERSITY   (Japan)

^b RIKEN HARIMA INSTITUTE   (Japan)

Author keywords

Cytochrome P450cam; Oxygen activation; Proton transfer; Site directed mutagenesis; X ray crystallography

Indexed keywords

ASPARTIC ACID; BACTERIAL ENZYME; CAMPHOR; CYTOCHROME P450; FERRIC ION; ISOLEUCINE; MUTANT PROTEIN; THREONINE; UNSPECIFIC MONOOXYGENASE; WATER;

ARTICLE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; PROTON TRANSPORT; STRUCTURE ACTIVITY RELATION; X RAY CRYSTALLOGRAPHY;

BINDING SITES; CAMPHOR; CAMPHOR 5-MONOOXYGENASE; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; ELECTRON TRANSPORT; ISOLEUCINE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OXYGEN; PROTEIN CONFORMATION; SPECTROPHOTOMETRY, ULTRAVIOLET; THREONINE; WATER;

BACTERIA (MICROORGANISMS); DRYOBALANOPS;

EID: 0034534875     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022848     Document Type: Article

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