Expression of type 1 5α-reductase and metabolism of testosterone in reconstructed human epidermis (SkinEthic®): A new model for screening skin-targeted androgen modulators

International Journal of Cosmetic Science

Volumn 22, Issue 6, 2000, Pages 397-407

  Roguet, R ^a,b   Cohen, C ^a,b   Leclaire, J ^a,b   Tessonneaud, E ^a,b   Gagne, C ^a,b   Teissier, M H ^a,b  

^a L ORÉAL RECHERCHE   (France)

^b Episkin SNC ZI des Trocques   (France)

Author keywords

5 reductase isoenzymes; Dihydrotestosterone; Reconstituted human epidermis; Screening model; Testosterone metabolism

Indexed keywords

ANDROSTANOLONE; FINASTERIDE; ISOENZYME; STEROID 5ALPHA REDUCTASE; TESTOSTERONE;

ARTICLE; CELL CULTURE; EPIDERMIS; GENE EXPRESSION; HORMONE ACTION; HUMAN; HUMAN CELL; MODEL; PROTEIN EXPRESSION; PROTEIN PROCESSING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; TESTOSTERONE METABOLISM; TRANSCRIPTION REGULATION;

EID: 0034509418     PISSN: 01425463     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1467-2494.2000.00035.x     Document Type: Article

References (21)

1. Selective retention of dihydrotestosterone by prostatic nuclei
2. The source of plasma dihydrotestosterone in man
3. A mutation that causes lability of the androgen receptor under conditions that normally promote transformation to the DNA-binding state
4. Genetic and pharmacological evidence for more than one human steroid 5α-reductase
5. Human genital melanocytes as androgen target cells
6. Dihydrotestosterone stimulates 5α-reductase activity in pubic skin fibroblasts
7. Evidence of heterogeneity and quantitative differences of the type 1, 5α-reductase expression in cultured human skin cells - Evidence of its presence in melanocytes
8. Identification and selective inhibition of an isozyme of steroid 5 alpha-reductase in human scalp
9. 17β-(N-tert-butylcarbomoyl)-4-aza-5α-androstan-1-en-3-one is an active site-directed slow time-dependent inhibitor of human steroid 5α-reductase 1
10. Avancées thérapeutiques en 1998, Dermatologie
11. Testosterone metabolism in an in vitro skin model
12. Cytochrome P450-dependent enzyme activities and testosterone metabolism in a reconstituted human epidermis
13. Terminal epidermal differentiation of human keratinocytes grown in chemically defined medium on inert filter substrates at the air-liquid interface
14. Retinoic acid inhibits epidermal differentiation when applied topically on the stratum corneum of epidermis formed in vitro by human keratinocytes grown on defined medium
15. Skin culture model: A possible alternative to the use of excised human skin for assessing in vitro percutaneous absorption
16. Use of reconstructed epidermis to assess keratinocyte activation by skin irritant and sensitizing compounds
17. Alternative methods for skin irritation testing: The current status. ECVAM Skin Irritation Task Force Report 1
18. Predictivity of an in vitro model for acute and chronic skin irritation (SkinEthic®) applied to the testing of topical vehicles
19. Differential expression and release of cytokines by an in vitro reconstituted human epidermis model following skin irritant and sensitizing compounds
20. Use of a serum-free reconstituted epidermis as a skin pharmacological model
21. UVA effects on in vitro reconstituted human epidermis (SkinEthic®): New developments in the determination of the phototoxic potential of cosmetic raw materials