Optimization of an estrogen receptor-α transcriptional activation assay for testing a diverse spectrum of chemicals

Journal of Applied Toxicology

Volumn 20, Issue 6, 2000, Pages 449-454

  Charles, G D ^a   Gennings, C ^b   Clemons, J ^c   Zacharewski, T R ^c   Gollapudi, B B ^a   Carney, E W ^a  

^a DOW CHEMICAL COMPANY   (United States)

^b VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^c Michigan State University   (United States)

Author keywords

Endocrine toxicity; Estrogenicity; MCF 7 cells; Osmolality; PH; Reporter gene; Solvent

Indexed keywords

BASE; DIMETHYL SULFOXIDE; ESTRADIOL; ESTROGEN RECEPTOR ALPHA; INDUSTRIAL CHEMICAL; INORGANIC ACID; LUCIFERASE; MANNITOL; SODIUM CHLORIDE; TYLOXAPOL;

ARTICLE; ASSAY; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ACTIVITY; ESTROGEN ACTIVITY; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HYPEROSMOLALITY; PH MEASUREMENT; PRIORITY JOURNAL; REPORTER GENE; TOXICITY; TOXICITY TESTING; TRANSACTIVATION;

CELLS, CULTURED; CHEMISTRY, PHYSICAL; ESTRADIOL; ESTRADIOL CONGENERS; ESTROGEN RECEPTOR ALPHA; FEMALE; GENES, REPORTER; HUMANS; HYDROGEN-ION CONCENTRATION; OSMOLAR CONCENTRATION; RECEPTORS, ESTROGEN; SOLVENTS; TRANS-ACTIVATION (GENETICS); TRANSFECTION;

EID: 0034508453     PISSN: 0260437X     EISSN: None     Source Type: Journal    
DOI: 10.1002/1099-1263(200011/12)20:6<449::AID-JAT712>3.0.CO;2-5     Document Type: Article

References (25)

1. Do environmental estrogens contribute to the decline in male reproductive health?
2. Endocrine effects of prenatal exposure to PCBs, dioxins and other xenobiotics; implications for policy and future research
3. Medical hypothesis: Xenoestrogens as preventable causes of breast cancer
4. The xenoestrogen Bisphenol A induces growth, differentiation, and c-fos gene expression in the female reproductive tract
5. Estrogenic and DNA-damaging activity of Red No. 3 in human breast cancer cells
6. Assessing the estrogenic and dioxin like activities of chemicals and complex mixtures using in vitro recombinant receptor-reporter gene assays
7. Persistent DDT metabolite p,p′-DDE is a potent androgen receptor antagonist
8. Dietary estrogens act through estrogen receptor mediated processes and show no antiestrogenicity in cultured breast cancer cells
9. Environmentally persistent alkylphenolic compounds are estrogenic
10. Nonylphenol: An estrogenic xenobiotic released from 'modified' polystyrene
11. Estrogenic action of DDT analogs
12. In vitro bioassays for assessing estrogenic substances
13. Identification and assessment of endocrine disruptors: Limitations of in vivo and in vitro assays
14. A potent specific pure antiestrogen with clinical potential
15. The metabolism of D- and L-pipecolic acid in the rabbit and rat
16. An interlaboratory comparison of enhanced morphological transformation of Syrian hamster embryo cells cultured under conditions of reduced bicarbonate concentration and pH
17. High-dose-level effects in mutagenicity assays utilizing mammalian cells in culture
18. Low cytoplasmic pH inhibits endocytosis and transport from the trans-Golgi network to the cell surface
19. Enhancement of chemotherapy by manipulation of tumour pH
20. An acidic environment leads to p53 dependent induction of apoptosis in human adenoma and carcinoma cell lines: Implications for clonal selection during colorectal carcinogenesis
21. The growth response of cells in medium made hyperosmolal with electrolytes or mannitol
22. Effect of mannitol on the permeability of cultured endothelial cells
23. Potentiating effects of hyper-osmolality and epidermal growth factor on the release of arachidonic acid in human glomerular mesangial cells