Ex vivo expansion of early and late megakaryocyte progenitors

Journal of Hematotherapy and Stem Cell Research

Volumn 9, Issue 6, 2000, Pages 913-921

  Lefebvre, P ^a   Winter, J N ^a   Meng, Y ^a   Cohen, I ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD34 ANTIGEN; FIBRINOGEN RECEPTOR; GRANULOCYTE COLONY STIMULATING FACTOR; MEGAKARYOCYTE GROWTH AND DEVELOPMENT FACTOR; STEM CELL FACTOR;

ARTICLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CLONOGENESIS; COLONY FORMING UNIT; FLOW CYTOMETRY; GROWTH STIMULATION; HUMAN; HUMAN CELL; MEGAKARYOCYTE; MEGAKARYOPOIESIS; PRIORITY JOURNAL; STEM CELL;

ANTIGENS, CD34; BREAST NEOPLASMS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CULTURE MEDIA, SERUM-FREE; FEMALE; GRANULOCYTE COLONY-STIMULATING FACTOR; HEMATOPOIETIC STEM CELLS; HUMANS; MEGAKARYOCYTES; PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX; STEM CELL FACTOR; THROMBOPOIETIN; TIME FACTORS;

EID: 0034486092     PISSN: 15258165     EISSN: None     Source Type: Journal    
DOI: 10.1089/152581600750062363     Document Type: Article

References (34)

1. Autologous peripheral hematopoietic stem cell transplantation restores hematopoietic function following narrow ablative therapy
2. Peripheral blood-derived stem cell collections for use in autologous transplantation after high dose chemotherapy: An alternative approach
3. Risks, costs, and alternatives to platelet transfusions
4. Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor mpl
5. Pegylated megakaryocyte growth and development factor abrogates the lethal thrombocytopenia associated with carboplatin and irradiation in mice
6. The structure, biology and potential therapeutic applications of recombinant thrombopoietin
7. Randomized, double-blind, placebo-controlled trial of pegylated recombinant human megakaryocyte growth and development factor in breast cancer patients following autologous bone marrow transplantation
8. Safety and biologic effect of pegylated recombinant human megakaryocyte growth and development factor in breast cancer patients following autologous peripheral blood progenitor cell transplantation
9. Effects of pegylated recombinant human megakaryocyte growth and development factor before high-dose chemotherapy with peripheral blood progenitor cell support
10. Safety and activity of recombinant human thrombopoietin in patients with delayed platelet recovery
11. A randomized, placebo-controlled, phase ii trial of recombinant human thrombopoietin (rhtpo) in subjects undergoing high dose chemotherapy (hdc) and pbpc transplant
12. Relationship of infused CFU-GM and CFU-mk mobilized by chemotherapy with or without g-csf to platelet recovery after autologous blood stem cell transplantation
13. The value of flow cytometric analysis of platelet glycoprotein expression of CD34+ cells measured under conditions that prevent p-selectin-mediated binding of platelets
14. CD34(+)/CD41(+) cells best predict platelet recovery after autologous peripheral blood stem cell transplantation
15. In vitro and in vivo evidence that ex vivo cytokine priming of donor marrow cells may ameliorate posttransplant thrombocytopenia
16. The mpl-ligand or thrombopoietin or megakaryocyte growth and differentiative factor has both direct proliferative and differentiative activities on human megakaryocyte progenitors
17. In vitro production of megakaryocytes from pixy321 versus GM-csf-mobilized peripheral blood progenitor cells
18. Thrombopoietin requires additional megakaryocyte-active cytokines for optimal ex vivo expansion of megakaryocyte precursor cells
19. Megakaryocytic progenitors can be generated ex vivo and safely administered to autologous peripheral blood progenitor cell transplant recipients
20. Abrogation of post-myeloablative chemotherapy neutropenia by ex-vivo expanded autologous CD34-positive cells
21. Ex vivo expanded peripheral blood progenitor cells provide rapid neutrophil recovery after high-dose chemotherapy in patients with breast cancer
22. High gradient magnetic cell separation with macs
23. Two-color flow cytometric measurement of DNA distributions of rat megakaryocytes in unfixed, unfractionated marrow cell suspensions
24. Increased megakaryocyte expansion with promegapoietin-progenipoietin combination and high seeding density
25. In vitro expansion of CD34+/CD41 + cells from human peripheral blood CD34+/CD41 - Cells: Role of cytokines for in vitro proliferation and differentiation of megakaryocytic progenitors
26. CD34+ cell positive selection from mobilized peripheral blood by an indirect immunomagnetic method: Effect of the type of mobilization and assessment of tumor depletion ability
27. CD34+ progenitor cell selection: Clinical transplantation, tumor cell purging, gene therapy, ex vivo expansion, and cord blood processing
28. Purified CD34+ lin- thy+ stem cells do not contain clonal myeloma cells
29. Collection, tumor contamination, and engraftment kinetics of highly purified hematopoietic progenitor cells to support high dose therapy in multiple myeloma
30. Megakaryocytopoiesis in the rat
31. The significance of megakaryocyte size
32. Unilineage megakaryocytic proliferation and differentiation of purified hematopoietic progenitors in serum-free liquid culture
33. Platelets generated in vitro from proplatelet-displaying human megakaryocytes are functional
34. Immunophenotype analysis of cytokine expanded CD34+ progenitor cells used for autologous transplantation