Investigation of nucleo-cytoplasmic transport using UV-guided microinjection

Journal of Cellular Biochemistry

Volumn 80, Issue 3, 2001, Pages 388-396

  Stauber, Roland H ^a   Krtzer, Friedrich ^a   Schneider, Grit ^a   Hirschmann, Nicole ^a   Hauber, Joachim ^a   Rosorius, Olaf ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

GFP BFP RFP; Lectins; M9; Microinjection; Nucleo cytoplasmic trafficking; Transcription inhibitors

Indexed keywords

LECTIN; PROTEIN;

ANIMAL CELL; ARTICLE; AUTOFLUORESCENCE; CELL INTERACTION; CELL MEMBRANE; CELL NUCLEUS MEMBRANE; CYTOPLASM; EUKARYOTE; FLUORESCENCE; MAMMAL; MICROINJECTION; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN TRANSPORT; TRANSCRIPTION TERMINATION;

MAMMALIA;

EID: 0034480097     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-4644(20010301)80:3<388::AID-JCB130>3.0.CO;2-V     Document Type: Article

References (43)

1. The human poly-A binding protein 1 shuttles between the nucleus and the cytoplasm
2. Definition of a consensus transportin-specific nucleocytoplasmic transport signal
3. Nucleocytoplasmic transport of macromolecules
4. Intracellular trafficking of the human immuno-deficiency virus type 1 Rev protein: Involvement of continued rRNA synthesis in nuclear retention
5. Inhibition of nuclear accumulation of karyophilic proteins in living cells by microinjection of the lectin wheat germ agglutinin
6. The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev protein
7. Translocation of RNA-coated gold particles through the nuclear pores of oocytes
8. Macromolecular exchanges between the nucleus and cytoplasm
9. Signal-mediated nuclear transport in the amoeba
10. Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores
11. Titration of cellular export factors, but not heteromultimerization, is the molecular mechanism of trans-dominant HTLV-1 Rex mutants
12. Interactions between the HIV Rev and nuclear import and export factors: The Rev nuclear localisation signal mediates specific binding to human importin-β
13. Transport of macromolecules between the nucleus and the cytoplasm
14. A role for the M9 transport signal of hnRNP A1 in mRNA nuclear export
15. The asymetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus
16. A short amino acid sequence able to specify nuclear location
17. Optical recording of signal-mediated protein transport through single nuclear pore complexes
18. Rev-dependent association of the intron-containing HIV-1 gag mRNA with the nuclear actin bundles and the inhibition of its nucleocytoplasmic transport by latrunculin-B
19. The adenovirus type 5 E1B-55K oncoprotein is a highly active shuttle protein and shuttling is independent of E4orf6, p53 and Mdm2
20. Single nuclear pores visualized by confocal microscopy and image processing
21. Structural dynamics of HIV-1 Rev and its complexes with RRE and 5S-RNA
22. Highly localized tracks of specific transcripts within interphase nuclei visualized by in situ hybridization
23. Transcription dependent nuclear/cytoplasmic trafficking is required for the function of the von Hippel-Lindau tumor suppressor protein
24. Nucleocytoplasmic transport: The soluble phase
25. Fluorescent proteins from nonbioluminiscent Antozoa species
26. Nopp140 shuttles on tracks between nucleolus and cytoplasm
27. The HIV-1 Rev trans-activator shuttles between the nucleus and the cytoplasm
28. The direction of transport through the nuclear pore can be inverted
29. Nuclear export of proteins and RNAs
30. Transportin: Nuclear transport receptor of a novel nuclear protein import pathway
31. In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins
32. Interactions and three-dimensional localization of a group of nuclear pore complex proteins
33. Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm
34. HIV-1 Rev is capable of shuttling between the nucleus and cytoplasm
35. RNA travel: Tracks from DNA to cytoplasm
36. Direct observation of nucleo-cytoplasmic transport by microinjection of GFP-tagged proteins in living cells
37. Three-dimensional visualization of the route of protein import: The role of nuclear pore complex substructures
38. Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins
39. Analysis of trafficking of Rev and transdominant Rev proteins in living cells using Green Fluorescent Protein fusions: Transdominant Rev blocks the export of Rev from the nucleus to the cytoplasm
40. Analysis of intracellular trafficking and interactions of cytoplasmic HIV-1 Rev mutants in living cells
41. Development and applications of enhanced Green Fluorescent Protein mutants
42. Nuclear protein import: Specificity for transport across the nuclear pore
43. Reversible inhibition of protein import into the nucleus by wheat germ agglutinin injected into cultured cells