Influence of preculture on the prefreeze and postthaw characteristics of hepatocytes

Biotechnology and Bioengineering

Volumn 71, Issue 3, 2000, Pages 173-183

  Hubel, A ^a   Conroy, M ^b   Darr, T B ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Cryopreservation; Hepatocyte; In vitro culture

Indexed keywords

CELL MEMBRANES; CHOLESTEROL; CONCENTRATION (PROCESS); LIPIDS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

CELL EXTRACTS;

BIOCHEMICAL ENGINEERING;

ALBUMIN; CELL EXTRACT; CHOLESTEROL; MEMBRANE PHOSPHOLIPID; TRIACYLGLYCEROL LIPASE;

ANIMAL CELL; ARTICLE; ARTIFICIAL LIVER; CELL CULTURE; CELL ISOLATION; CELL VIABILITY; CRYOPRESERVATION; LIVER CELL; MALE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SWINE; THAWING; WATER PERMEABILITY;

ALBUMINS; ANIMALS; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CELL SURVIVAL; CELLS, CULTURED; CRYOPRESERVATION; CULTURE MEDIA; HEPATOCYTES; LIVER; MAGNETIC RESONANCE SPECTROSCOPY; MALE; PHOSPHOLIPIDS; SWINE;

EID: 0034447223     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(2000)71:3<173::AID-BIT1007>3.0.CO;2-2     Document Type: Article

References (43)

1. Albi E, Peloso I, Magni MV. 1999. Nuclear membrane sphingomyelin-cholesterol changes in rat liver after hepatectomy. Biochem Biophys Res Commun 262:692-695.
2. 1H-NMR investigation in vitro of normal and abnormal human liver. Biochim Biophys Acta 1225: 71-77.
3. Borel Rinkes I, Toner M, Sheehan S, Tompkins R, Yarmush M. 1992. Long-term functional recovery of hepatocytes after cryopreservation in a three-dimensional culture configuration. Cell Transplantation 1: 281-292.
4. Changani KK, Ala-Korpela M, Fuller BJ, Mierisova S, Bryant DJ, Taylor-Robinson SD, Davidson BR, Bell JD. 1999. Incorporation of metabolite prior knowledge for data analysis: biochemical implications of dynamic 31P NMR ex vivo pig liver studies. NMR Biomed 12:197-204.
5. 31P-NMR study. Transplantation 62:787-793.
6. Chi Y, Gupta RK. 1998. Alterations in membrane fatty acid unsaturation and chain length in hypertension as observed by 1H NMR spectroscopy. Am J Hypertens 11:340-348.
7. Dagnelie PC, Sijens PE, Kraus DJA, Planting AS, van Dijk PV. 1999. Abnormal liver metabolism in cancer patients detected by 31P MR spectroscopy. NMR Biomed 12:535-544.
8. Darr T, Hubel A. Postthaw viability of precultured hepatocytes. Cryobiology, submitted.
9. Dixit V, Darvasi R, Arthur M, Lewin K, Gitnick G. 1993. Cryopreserved microencapsulated hepatocytes - transplantation studies in Gunn rats. Transplantation. 55(3):616-622.
10. Dunn JC, Friedberg JS, Tompkins RG, Yarmush ML. 1992. Hepatocytes from rat liver perfusions. Physicochemical effects on polyribosome size. ASAIO Journal 38(4):841-845.
11. 1H NMR study of membrane phospholipids in rat pancreas stimulated by caerulein. Pancreas 9:263-269.
12. 31P NMR measurements of the effects of unsaturated fatty acids on cellular phospholipid metabolism. Magn Reson Med 35:481-488.
13. 31P nuclear magnetic resonance spectroscopy study. Arch Dermatol Res 290:298-305.
14. Guillouzo A, Rialland L, Fautrel A, Guyomard C. 1999. Survival and function of isolated hepatocytes after cryopreservation. Chemico-Biolog Interact 121:7-16.
15. Guyomard C, Rialland L, Fremond B, Chesne C, Guillouzo A. 1996. Influence of alginate gel entrapment and cryopreservation on survival and xenobiotic metabolism capacity of rat hepatocytes. Toxicol Appl Pharmacol 141(2):349-356.
16. 1H NMR spectroscopic studies of liver extracts of carbon tetrachloride-treated rats. NMR Biomed 12:395-401.
17. Hubel A, Toner M, Cravalho EG, Yarmush ML, Tompkins RG. 1991. Intracellular ice formation during the freezing of hepatocytes cultured in a double collagen gel. Biotech Prog 7:554-559.
18. Kasai S, Mito M. 1993. Large-scale cryopreservation of isolated dog hepatocytes. Cryobiology 30:1-11.
19. Koebe H, Dahnhardt C, Muller-Hocker J, Wagner H, Schildberg F. 1996. Cryopreservation of porcine hepatocyte cultures. Cryobiology 33: 127-141.
20. Lazar A, Peshwa MV, Wu FJ, Chi CM, Cerra FB, Hu WS. 1995a. Formation of porcine hepatocyte spheroids for use in bioartificial liver. Cell Transplant 4(3):259-268.
21. Lazar A, Mann HJ, Remmel RP, Sharford RP, Cerra FB, Hu WS. 1995b. Extended liver-specific function of porcine hepatocyte spheroids entrapped in collagen gel. In vitro Cellular and Dev Biol 31(5):340-346.
22. Lehninger AL. 1975. Biochemistry. Worth Publishers, New York.
23. 31P magnetic resonance spectroscopic studies of the hepatic response of healthy rats and rats with acute hepatic damage to fructose loading. Mag Reson Med 31:469-481.
24. Males RG, Herring FG. 1999. A 1H-NMR study of the permeation of glycolic acid through phospholipid membranes. Biochim Biophys Acta 1416:333-338.
25. Meneses P, Glonek T. 1988. High resolution 31P NMR of extracted phospholipids. J Lipid Res 29:679-689.
26. 31P nuclear magnetic resonance phospholipid and phosphatic metabolite profiles. Cancer 15:1715-1723.
27. 31P NMR study. J Lipid Res 40:515-521.
28. Nouri-Sorkhabi MH, Chapman BE, Sullivan DR, Kuchel PW. 1994. Cholesterol effects on nonelectrolyte membrane transport in human erythrocytes: NMR magnetization transfer studies. Magn Reson Med 32: 505-510.
29. Nyberg SL, Shirabe K, Peshwa MV, Sielaff TD, Crotty PL, Mann HJ, Remmel RP, Payne WD, Hu WS, Cerra FB. 1993. Extracorporeal application of a gel-entrapment, bioartificial liver: Demonstration of drug metabolism and other biochemical functions. Cell Transplantation 2(6):441-152.
30. Peshwa MV, Wu FJ, Follstad BD, Cerra FB, Hu WS. 1994. Kinetics of hepatocyte spheroid formation. Biotechnol Progr 10:460-466.
31. 1H NMR spectroscopic studies of lipid extracts from human fatty liver. Biochem Biophys Res Comm 192: 1217-1222.
32. Pulkkinen M, Nyman S, Hamalainen MM, Mattinen J. 1998. Proton NMR spectroscopy of the phospholipids in human uterine smooth muscle and placenta. Gynecol Obstet Invest 46:220-224.
33. Seglen PO. 1976. Preparation of rat liver cells. Method Cell Biol 13:29-38.
34. Tomassoni ML, Albi E, Magni MV. 1999. Changes of nuclear membrane fluidity during rat liver regeneration. Biochem Mol Biol Intl 47: 1049-1059.
35. Tong JZ, De LP, Furlan V, Cresteil T, Bernard O, Alvarez F. 1992. Long-term culture of adult rat hepatocyte spheroids. Exp Cell Res 200: 326-332.
36. Toussaint O, Fuch S, Ronai A, Isoyama S, Yuko N, Petronilli V, Bernardi P, Gonos E, Dumont P, Remacle J. 1998. Reciprocal relationship between the resistance to stresses and cellular aging. Ann NY Acad Sci 851:450-465.
37. Toussaint O, Michiels C, Raes M, Remacle J. 1995. Cellular aging and the importance of energetic factors. Exp Gerontol 30:1-22.
38. Toussaint O, Houbion A, Remacle J. 1994. Effects of modulations of the energetic metabolism on the mortality of cultured cells. Biochim Biophys Acta 1186:209-220.
39. Tyagi RK, Azrad A, Degani H, Salomon Y. 1996. Simultaneous extraction of cellular lipids and water soluble metabolites: Evaluation by NMR spectroscopy. Magn Reson Med 35:194-200.
40. Voet D, Voet J. Biochemistry. 1995. New York: John Wiley & Sons.
41. Waldeck RA, Nouri-Sorkhabi MH, Sullivan DR, Kuchel PW. 1995. Effects of cholesterol on transmembrane water diffusion in human erythrocytes measured using pulsed field gradient NMR. Biophys Chem 55: 197-208.
42. 31P MRS. Magn Reson Med 40:411-420.
43. Yarmush ML, Toner M, Dunn J, Rotem A, Hubel A, Tompkins R. 1992. Hepatic tissue engineering: Development of critical technologies. Ann NY Acad Sci 665:238-252.