Structural Arrangement for Functional Requirements of Brain Recombinant 4-Aminobutyrate Aminotransferase

Journal of Biochemistry and Molecular Biology

Volumn 33, Issue 1, 2000, Pages 43-48

  Sung, Bo Kyung ^a   Kim, Young Tae ^a  

^a Pukyong National University   (South Korea)

Author keywords

4 Aminobutyrate aminotransferase; Dimeric structure; Energy transfer; Fluorescence; Stability

Indexed keywords

EID: 0034416476     PISSN: 12258687     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Beeler, T. and Churchich, J. E. (1978) Purification and characterization of pig brain 4-aminobutyrate aminotransferase. Eur. J. Biochem. 85, 365-371.
2. Bradford, M. M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
3. Cho, J. J., Kim, S. K. and Kim, Y. T. (1997) Catalytic and structural properties of pyridoxal kinase. J. Biochem. Mol. Biol. 30, 125-131.
4. Choi, S. Y. and Churchich, J. E. (1985) 4-aminobutyrate aminotransferase. Conformational changes induced by reduction of pyridoxal 5-phosphate. Biochim. Biophys. Acta 830, 120-126.
5. Churchich, J. E. (1976) in Modern Fluorescence spectroscopy: Fluorescent probe studies of binding sites in proteins and enzymes, Wehry, E. (ed.), pp217-237, Plenum Press, New York.
6. Churchich, J. E. (1982) 4-aminobutyrate aminotransferase. Different susceptibility to inhibitors, microenvironment of the cofactor binding sites. Eur. J. Biochem. 126, 507-511.
7. Churchich, J. E. and Moses, U. (1981) 4-aminobutyrate aminotransferase. The presence of nonequivalent binding sites. J. Biol. Chem. 256, 1101-1104.
8. Kim, H. (1999) Protein engineering of an artificail intersubunit disulfide bond linkage in human dihydrolipoamide dehydrogenase. J. Biochem. Mol. Biol. 32, 76-81.
9. Kim, Y. T. and Churchich, J. E. (1989) Sequence of the cysteinyl containing peptides of 4-aminobutyrate aminotransferase: Identification of sulfhydryl residues involved in intersubunit linkage. Eur. J. Biochem. 181, 397-401.
10. Kim, Y. T. and Churchich, J. E. (1991) 4-aminobutyrate aminotransferase: Identification of Lysyl residues connected with catalytic activity. Biochim. Biophys. Acta. 1077, 187-191.
11. Kim, Y. T. and Richardson, C. C. (1993) Bacteriophage T7 gene 2.5 protein: an essential protein for DNA replication. Proc. Natl. Acad. Sci. U. S. A. 90, 10173-10177.
12. Kim, Y. T. and Richardson, C. C. (1994) Acidic Carboxyl-terminal domain of gene 2.5 protein of bacteriophage T7 is essential for protein-protein interactions. J. Biol. Chem. 269, 5270-5278.
13. Kim, Y. T., Lee, S. G. and Kim, H. J. (1995) Molecular and Biochemical Studies on the DNA Replication of Bacteriophage T7: Functional Analysis of Amino-terminal Region of Gene 2.5 Protein. J. Biochem. Mol. Biol. 28, 484-489.
14. Kim, Y. T., Park, J. H., Choi, S. Y., Kwon, O. and Churchich, J. E. (1991) Isolation and characterization of cDNA coding for 4-aminobutyrate aminotransferase; in Carbonyl catalysis, Fukui, T., Kagamiyama, H., Soda, K. and Wada, H. (eds.), pp 63-67, Pergamon Press, New York.
15. Kim, Y. T., Song, Y. H. and Churchich, J. E. (1997) Recombinant brain 4-aminobutyrate aminotransferase -Overexpression, purification and identification of 330 Lys at the active site. Biochim. Biophys. Aata. 1377, 248-256.
16. Kim, Y. T., Tabor, S., Bortner, C., Griffith, J. D. and Richardson, C. C. (1992b) Purification and characterization of bacteriophage T7 gene 2.5 protein: a single-stranded DNA binding protein. J. Biol. Chem. 267, 15022-15031.
17. Kim, Y. T., Tabor, S., Churchich, J. E. and Richardson, C. C. (1992a) Interaction of the gene 2.5 protein and DNA polymerase of bacteriophage T7. J. Biol. Chem. 267, 15032-15040.
18. Kwok, F., Scholz, G. and Churchich, J. E. (1987) Brain pyridoxal kinase dissociation of the dimeric structure and catalytic activity of the monomeric species. Eur. J. Biochem., 168, 577-578.
19. Kwon, O., Park, J. and Churchich, J. E. (1992) Brain 4-aminobutyrate aminotransferase. J. Biol. Chem. 267, 7215-7216.
20. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 277, 680-685.
21. Lakowicz, J. R. (1983) In principles of fluorescent spectroscopy, pp.342-379, Plenum Press, New York..
22. Lee, S. G., Choi, T. J. and Kim, Y. T. (1996) High-level expression of recombinant 4-aminobutyrate aminotransferase. J. Microbiol. Biotech. 9, 453-459.
23. Park, J., Osei, Y. D. and Churhich, J. E. (1993) Isolation and characterization of recombinant mitochondrial 4-aminobutyrate aminotransferase. J. Biol. Chem. 268, 7636-7639.
24. Pickover, C. A., McKay, D. B., Engelman, D. M. and Steitz, T. A. (1979) Substrate binding closes the cleft between the domains of yeast phosphoglycerate kinase. J. Biol. Chem. 214, 13323-13330.
25. Sung, B. K., Cho, J. J. and Kim, Y. T. (1999) Functional expression and characterization of C-terminal mutant of 4-aminobutyrate aminotransferase. J. Biochem. Mol. Biol. 32, 181-188.
26. Tabor, S. and Richardson, C. C. (1985) A bacteriophage T7 RNA polymerase/promoter system for the controlled: exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82, 1074-1078.
27. Yasukawa, T., Kanei-Ishii, C., Maekawa, T., Fujimoto, J., Yamamoto, T., and Ishii, S. (1995) Increase of solubility of foreign proteins in Escherichia coli by coproduction of the bacterial thioredoxin. J. Biol. Chem. 270, 25328-25331.