Recombinant brain 4-aminobutyrate aminotransferases overexpression, purification, and identification of Lys-330 at the active site

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1337, Issue 2, 1997, Pages 248-256

  Kim, Young Tae ^a   Song, Young Hwan ^a   Churchich, Jorge E ^b  

^a Pukyong National University   (South Korea)

^b UNIVERSITY OF TENNESSEE   (United States)

Author keywords

(Porcine brain); 4 Aminobutyrate aminotransferase; Mutagenesis; Overexpression; Purification; Pyridoxal 5' P

Indexed keywords

4 AMINOBUTYRATE AMINOTRANSFERASE; AMINOTRANSFERASE; COMPLEMENTARY DNA; MUTANT PROTEIN; RECOMBINANT ENZYME;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; BRAIN; CELL INCLUSION; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE OVEREXPRESSION; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SPECTROPHOTOMETRY; SWINE;

4-AMINOBUTYRATE TRANSAMINASE; ANIMALS; BASE SEQUENCE; BINDING SITES; BRAIN; DNA PRIMERS; DNA, COMPLEMENTARY; GENETIC VECTORS; LYSINE; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; SWINE;

ANIMALIA; ESCHERICHIA COLI; SUIDAE; SUS SCROFA;

EID: 0031031867     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(96)00171-9     Document Type: Article

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