Interleukin-1β induces different gene expression of stromelysin, aggrecan and tumor-necrosis-factor-stimulated gene 6 in human osteoarthritic chondrocytes in vitro

Pathobiology

Volumn 68, Issue 3, 2000, Pages 144-149

  Stöve, Johannes ^a,b   Huch, Klaus ^a   Günther, Klaus Peter ^a   Scharf, Hanns Peter ^a  

^a UNIVERSITY OF ULM   (Germany)

^b Rehabilitationskrankenhaus Ulm gGmbH   (Germany)

Author keywords

Aggrecan; Chondrocyte; Gene expression; IL 1 ; Stromelysin; TSG 6

Indexed keywords

AGGRECAN; INTERLEUKIN 1BETA; STROMELYSIN; TUMOR NECROSIS FACTOR STIMULATED GENE 6 PROTEIN; UNCLASSIFIED DRUG; CELL ADHESION MOLECULE; DNA; INTERLEUKIN 1; LECTIN; MESSENGER RNA; PRIMER DNA; PROTEOGLYCAN; SCLEROPROTEIN; TNFAIP6 PROTEIN, HUMAN;

AGED; ARTICLE; CARTILAGE CELL; CLINICAL ARTICLE; CONTROLLED STUDY; FEMALE; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; HUMAN TISSUE; MALE; OSTEOARTHRITIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE DIAGNOSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSCRIPTION REGULATION; ARTICULAR CARTILAGE; CELL CULTURE; CHEMISTRY; CYTOLOGY; DRUG EFFECT; GENE EXPRESSION; GENETICS; KNEE OSTEOARTHRITIS; METABOLISM; PATHOLOGY; REPRODUCIBILITY;

AGED; AGGRECANS; CARTILAGE, ARTICULAR; CELL ADHESION MOLECULES; CELLS, CULTURED; CHONDROCYTES; DNA; DNA PRIMERS; EXTRACELLULAR MATRIX PROTEINS; FEMALE; GENE EXPRESSION; HUMANS; INTERLEUKIN-1; LECTINS, C-TYPE; MALE; MATRIX METALLOPROTEINASE 3; OSTEOARTHRITIS, KNEE; PROTEOGLYCANS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 0034351876     PISSN: 10152008     EISSN: None     Source Type: Journal    
DOI: 10.1159/000055915     Document Type: Article

References (25)

1. Adult human chondrocytes cultured in alginate form a matrix similar to native human articular cartilage
2. Expression of a stable articular cartilage phenotype without evidence of hypertrophy by adult human articular chondrocytes in vitro
3. Dedifferentiated chondrocytes cultured in alginate beads: Restoration of the differentiated phenotype and of the metabolic responses to interleukin-1beta
4. Complexity of IL-1 beta induced gene expression pattern in human articular chondrocytes
5. Effects of fibronectin on articular cartilage chondrocyte proteoglycan synthesis and response to insulin-like growth factor-I
6. Matrix degradation by chondrocytes cultured in alginate: IL-1 beta induces proteoglycan degradation and proMMP synthesis but does not result in collagen degradation
7. The role of cytokines as inflammatory mediators in osteoarthritis: Lessons from animal models
8. In vitro effects of interleukin 1 on the synthesis of metalloproteases, TIMP, plasminogen activators and inhibitors in human articular cartilage
9. The interleukin-1 receptor in normal and osteoarthritic human articular chondrocytes. Identification as the type I receptor and analysis of binding kinetics and biologic function
10. Quantification of aggrecan and link-protein mRNA in human articular cartilage of different ages by competitive reverse transcriptase-PCR
11. Biochemical and molecular characterization of stromelysin synthesized by human osteoarthritic chondrocytes stimulated with recombinant human interleukin-1
12. TSG-6 expression in human articular chondrocytes. Possible implications in joint inflammation and cartilage degradation
13. TNF/IL-1-inducible protein TSG-6 potentiates plasmin inhibition by inter-alpha-inhibitor and exerts a strong anti-inflammatory effect in vivo
14. Synthesis and turnover of proteoglycans by human and bovine adult articular chondrocytes cultured in alginate beads
15. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction
16. Rapid quantitative PCR determination of relative gene expression in tumor specimens using high-pressure liquid chromatography
17. Interleukin-1 beta-modulated gene expression in immortalized human chondrocytes
18. Metalloproteinases, tissue inhibitor, and proteoglycan fragments in knee synovial fluid in human osteoarthritis
19. In vivo expression of stromelysin in synovium and cartilage of rabbits injected intraarticularly with interleukin-1 beta
20. Structure of the human aggrecan gene: Exon-intron organization and association with the protein domains
21. Transcriptional suppression by interleukin-1 and interferon-gamma of type II collagen gene expression in human chondrocytes
22. Phenotypic modulation of chondrocytes as a potential therapeutic target in osteoarthritis: A hypothesis
23. A novel secretory tumor necrosis factor-inducible protein (TSG-6) is a member of the family of hyaluronate binding proteins, closely related to the adhesion receptor CD44
24. TSG-6, an arthritis-associated hyaluronan binding protein, forms a stable complex with the serum protein inter-alpha-inhibitor
25. Accurate accuracy assessment: Review of basic principles