Sequence-selective RNA scission by non-covalent combination of acridine-tethered DNA and lanthanide(III) ion

Chemistry Letters

Volumn , Issue 12, 2000, Pages 1378-1379

  Kuzuya, Akinori ^a   Komiyama, Makoto ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0034345666     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2000.1378     Document Type: Article

References (19)

1. a) B. N. Trawick, A. T. Daniher, and J. K. Bashkin, Chem. Rev., 98, 939 (1998).
2. b) M. Oivanen, S. Kuusela, and H. Lönnberg, Chem. Rev., 98, 961 (1998).
3. c) M. Komiyama, N. Takeda, and H. Shigekawa, Chem. Commun., 1999, 1443.
4. a) K. Matsumura, M. Endo, and M. Komiyama, J. Chem. Soc., Chem. Commun., 1994, 2019.
5. b) M. Komiyama, T. Inokawa, and K. Yoshinari, J. Chem. Soc., Chem. Commun., 1995, 77.
6. c) J. Hovinen, A. Guzaev, E. Azhayeva, A. Azhayev, and H. Lönnberg, J. Org. Chem., 60, 2205 (1995).
7. d) M. A. Reynolds, T. A. Beck, P. B. Say, D. A. Schwartz, B. P. Dwyer, W. J. Daily, M. M. Vaghefi, M. D. Metzler, R. E. Klem, and L. J. Arnold, Jr., Nucleic Acids Res., 24, 760 (1996).
8. e) J. Hall, D. Hüsken, and R. Häner, Nucleosides Nucleotides, 16, 1357 (1997).
9. f) D. Magda, M. Wright, S. Crofts, A. Lin, and J. L. Sessler, J. Am. Chem. Soc., 119, 6947 (1997).
10. g) S. Matsuda, A. Ishikubo, A. Kuzuya, M. Yashiro, and M. Komiyama, Angew. Chem., Int. Ed., 37, 3284 (1998).
11. h) W. C. Putnam and J. K. Bashkin, Chem. Commun., 2000, 767.
12. A pioneering work on non-covalent systems for site-selective RNA hydrolysis was reported: D. Hüsken, G. Goodall, M. J. J. Blommers, W. Jahnke, J. Hall, R. Häner, and H. E. Moser, Biochemistry, 35, 16591 (1996).
13. A. Kuzuya, M. Akai, and M. Komiyama, Chem. Lett., 1999, 1035.
14. A. Kuzuya and M. Komiyama, Polymer Preprints, Japan, 49, 981 (2000).
15. At the reaction time 2 h, the conversions for the scission at C-18 and U-19 were 12 and 3 mol%, respectively.
16. The scission sites by this ternary system are also C-18 and U-19.
17. 2-Acr, the scission was much less efficient (lane 2). An essential role of the acridine is also evident in the binary systems.
18. This system is one of the most active site-selective RNA-cleaving agents that ever reported (ref 5).
19. By promoting the activating effect still more, longer RNA should be able to be hydrolyzed at the target site.