Hydrolysis of oligonucleotides by homogeneous Ce(IV)/EDTA complex

Chemistry Letters

Volumn , Issue 4, 2000, Pages 356-357

  Igawa, Tomoyuki ^a   Sumaoka, Jun ^a   Komiyama, Makoto ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0034341138     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2000.356     Document Type: Article

References (28)

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18. The mechanism of Ce(IV)-induced hydrolysis of DNA was recently discussed; M. Komiyama, N. Takeda, and H. Shigekawa, J. Chem. Soc., Chem. Commun, 1999, 1443.
19. Artificial enzymes for sequence-selective scission of DNA were prepared by conjugating Ce(IV) ion with DNA oligomers: a) M. Komiyama, T. Shiiba, Y. Takahashi, N. Takeda, K. Matsumura, and T. Kodama, Supramol. Chem., 4, 31 (1994).
20. M. Komiyama, J. Biochem., 118, 665 (1995).
21. Plasmid DNA was chosen as a substrate because of the experimental convenience (the products were easily analyzed by agarose-gel electrophoresis): a) J. Sumaoka, T. Igawa, and M. Komiyama, Chem. Lett., 2000, 56.
22. M.E. Branum and L. Que, Jr. independently reported that plasmid DNA is hydrolyzed by a dinuclear Ce(IV) complex of 1,3-diamino-2-hydroxypropane-N,N,N′,N′-tetraacetate: J. Inorg. Biochem., 74, 81 (1999).
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24. -3 ammonium formate.
25. 4 were also hydrolyzed rather efficiently, all the products were well characterized by the HPLC and thus the efficiency for the scission of the internal linkages could be precisely evaluated.
26. 3p. The first six are clearly observed in Figure 3.
27. When the reaction mixtures were treated with hot-piperidine (90 °C for 30 min), the polyacrylamide-gel electrophoresis patterns were virtually unchanged. No alkaline-labile sites were formed during the reactions, providing a further evidence for the hydrolytic nature of the scission.
28. Similar chelate-structures were previously proposed in the metal-mediated hydrolysis of DNA (Ref. 4d) and RNA (S. Kuusela, A. Guzaev, and H. Lönnberg, J. Chem. Soc., Perkin Trans. 2, 1996, 1895).