Homogeneous Ce(IV) complexes for efficient hydrolysis of plasmid DNA

Chemistry Letters

Volumn , Issue 1, 2000, Pages 56-57

  Sumaoka, Jun ^a   Igawa, Tomoyuki ^a   Furuki, Kenichiro ^a   Komiyama, Makoto ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0034340841     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2000.56     Document Type: Article

References (25)

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4. A recent account on the mechanism of the lanthanide ion-mediated hydrolysis of DNA and RNA; M. Komiyama, N. Takeda, and H. Shigekawa, J. Chem. Soc., Chem. Commun, 1999, 1443.
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20. In the absence of the polymers, the Tm(III) is one of the most active catalysts for the hydrolysis of the plasmid DNA. The PVP did not much alter its activity.
21. J. Sumaoka, A. Kajimura, M. Ohno, and M. Komiyama, Chem. Lett., 1997, 507.
22. The activity of Ce(IV)/EDTA is lower than those of the other two homogeneous system probably because of the negative charges of the EDTA. The positive charge on Ce(IV) is mostly compensated by EDTA. The positive charge electrostatically stabilizes the negatively charged transition state for DNA hydrolysis.
23. Assumedly, the Ce(IV)-hydroxide gel cannot get close to the phosphodiester linkages in the plasmid DNA, which are exceptionally reactive and more easily hydrolyzed than the others.
24. -3) is 3 fold less active for the hydrolysis of TpT than is the corresponding Ce(IV)-hydroxide gel. In this small substrate, the steric hindrance is not so significant as in the hydrolysis of plasmid DNA and oligonucleotides.
25. Interestingly, the Ce(IV)/EDTA complex is virtually inactive for the hydrolysis of dinucleotides, although it efficiently hydrolyzes the plasmid DNA and the oligonucleotides. Detailed study on these points is in progress.