DNA sequence of the insertional hot spot of Tn916 in the Clostridium difficile genome and discovery of a Tn916-like element in an environmental isolate integrated in the same hot spot

FEMS Microbiology Letters

Volumn 192, Issue 1, 2000, Pages 15-20

  Wang, Hongmei ^a   Roberts, Adam P ^a   Mullany, Peter ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Clostridium difficile; Conjugative transposon; Tn916

Indexed keywords

BACTERIAL DNA; PLASMID DNA; TETRACYCLINE;

ANTIBIOTIC RESISTANCE; ARTICLE; CLOSTRIDIUM DIFFICILE; DNA SEQUENCE; GENOME; NONHUMAN; PRIORITY JOURNAL; TRANSPOSON;

BACILLUS SUBTILIS; BASE SEQUENCE; CLOSTRIDIUM DIFFICILE; CONJUGATION, GENETIC; DNA TRANSPOSABLE ELEMENTS; ENVIRONMENTAL MICROBIOLOGY; GENOME, BACTERIAL; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SUPPORT, NON-U.S. GOV'T;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); CLOSTRIDIUM DIFFICILE;

EID: 0034333343     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00401-8     Document Type: Article

References (23)

1. Evidence for a chromosome borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of 'conjugal' transfer in the absence of a plasmid
2. Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposons
3. Conjugative transposition
4. Tn916 family of conjugative transposons and dissemination of antimicrobial resistance determinants
5. Molecular characterisation of two proteins involved in the excision of the conjugative transposon Tn1545: homologies with other site-specific recombinases
6. Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon encoded integrase
7. Regulation of excision of the conjugative transposon Tn916
8. The integration-excision system of the conjugative transposon Tn1545 is structurally and functionally related to those of the lambdoid phages
9. The joint of Tn916 circular intermediates is a homoduplex in Enterococcus faecalis
10. Gene cloning in Clostridium difficile using Tn916 as a shuttle conjugative transposon
11. Insertion vectors for construction of recombinant conjugative transposons in Bacillus subtilis and Enterococcus faecalis
12. Sequence requirements for target activity in site-specific recombination mediated by the Int protein of transposon Tn1545
13. Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate
14. Transfer of Tn916 and Tn916ΔE into Clostridium difficile: demonstration of a hot-spot for these elements in the C. difficile genome
15. Characterisation of the ends and target sites of the novel conjugative transposon Tn5397 from Clostridium difficile: Demonstration that excision and circularisation is mediated by TndX, a member of the large resolvase family
16. A Group II intron in a conjugative transposon from the Gram-positive bacterium, Clostridium difficile
17. Nucleotide sequence of the 18 kb conjugative transposon Tn916 from Enterococcus faecalis
18. Evidence that coupling sequences play a frequency determining role in conjugative transposition of Tn916 in Enterococcus faecalis
19. Coupling sequences flanking Tn916 do not determine the affinity of binding of integrase to the transposon ends and adjacent bacterial DNA
20. Circularisation of Tn916 is required for expression of the transposon-encoded transfer functions: characterisation of long tetracycline-induciable transcripts reading through the attachment site
21. Two conjugation systems associated with Streptococcus faecalis plasmid pCF10: identification of a conjugative transposon that transfers between S. faecalis and Bacillus subtilis
22. Homology of a transferable tetracycline resistance determinant of Clostridium difficile with Streptococcus (Enterococcus) faecalis transposon Tn916
23. Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in E. coli: strategy for targeting and cloning genes from Gram positive bacteria