Europium cryptate-tethered ribonucleotide for the labeling of RNA and its detection by time-resolved amplification of cryptate emission

Analytical Biochemistry

Volumn 286, Issue 1, 2000, Pages 17-25

  Alpha Bazin, Béatrice ^a   Bazin, Hervé ^a   Boissy, Lilian ^a   Mathis, Gérard ^a  

^a CIS Bio International   (France)

Author keywords

Energy transfer; Eu3+ cryptate; FRET; Nonisotopic RNA labeling; Time resolved fluorescence

Indexed keywords

COENZYMES; ENERGY TRANSFER; EUROPIUM; FORSTER RESONANCE ENERGY TRANSFER;

ENZYMATIC SYNTHESIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FRET; HOMOGENEOUS DETECTIONS; HYBRIDIZATION ASSAY; PHARMACEUTICAL APPLICATIONS; TIME-RESOLVED FLUORESCENCE; URIDINE TRIPHOSPHATE;

RNA;

ALLOPHYCOCYANIN; BIOTIN; EUROPIUM TRISBIPYRIDINE CRYPTATE; METAL DERIVATIVE; RNA; RNA POLYMERASE; STREPTAVIDIN; UNCLASSIFIED DRUG; URIDINE TRIPHOSPHATE;

ANALYTIC METHOD; ARTICLE; DNA RNA HYBRIDIZATION; ENERGY TRANSFER; MOLECULAR INTERACTION; PRIORITY JOURNAL; RNA ANALYSIS;

EID: 0034327261     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4764     Document Type: Article

References (34)

1. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements
2. Bacteriophage SP6-specific RNA polymerase. II. Mapping of SP6 DNA and selective in vitro transcription
3. dNTP modified at triphosphate residues: Substrate properties towards DNA polymerases and stability in human serum
4. Nuclear runoff transcription analysis using chemiluminescent detection
5. Degree of biotinylation in nucleic acids estimated by a gel retardation assay
6. Simultaneous visualization of seven different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy
7. DNA sequence localization in metaphase and interphase cells by fluorescence in situ hybridization
8. Detection of HIV-1 DNA by polymerase chain reaction incorporation of digoxigenin-11-dUTP and hybridization to immobilized probes
9. Nucleosides Nucleotides
10. An automated high-throughput filtration assay: Application to polymerase inhibitor identification
11. Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG); hybridization and ELISA-based detection
12. Fluorescence in situ hybridization with Alu and L1 polymerase chain reaction probes for rapid characterization of human chromosomes in hybrid cell lines
13. Cyanine dye dUTP analogs for enzymatic labelling of DNA probes
14. Gene probe assays and their detection
15. Rare earth cryptates and homogeneous fluoroimmunoassays with human sera
16. Probing molecular interactions with homogeneous technique based on rare earth cryptates and fluorescence energy transfer
17. Zwischen molekulare energiewanderung und fluoreszenz
18. Fluorescence energy transfer as a spectroscopic ruler
19. Energy transfer luminescence of Eu(III) and Tb(III) cryptates of macrobicyclic polypyridine ligands
20. Enzymatic synthesis of biotin-labeled polynucleotides: Novel nucleic acid affinity probes
21. Photoactive cryptands
22. Rapid and large-scale method to detect K-ras gene mutations in tumor samples
23. Fast and reliable screening of mutations in human tumors: Use of multiple fluorescence-based long linker arm nucleotides assay (mfLLA)
24. Synthesis and use of labeled nucleoside phosphoramidite building blocks bearing a reporter group: Biotinyl, dinitrophenyl, pyrenyl and dansyl
25. The use of heterocyclic chemistry in the synthesis of natural and unnatural products
26. Fast chemiluminescent measurement of RNA polymerase activity based on photon counting technology
27. Inhibition of RNA polymerases by digoxigenin-labeled UTP