Rapid and large-scale method to detect K-ras gene mutations in tumor samples

Clinical Chemistry

Volumn 43, Issue 6, 1997, Pages 936-942

  Lopez Crapez, Evelyne ^a   Chypre, Camille ^b   Saavedra, Jacqueline ^b   Marchand, Joseph ^b   Grenier, Jean ^a  

^a CENTRE VAL D AURELLE   (France)

^b CIS Bio International   (France)

Author keywords

Allele specific oligonucleotide detection; Immobilized oligonucleotide probes; Point mutation; Polymerase chain reaction

Indexed keywords

2,4 DINITROPHENOL; DNA; IODINE 125; MONOCLONAL ANTIBODY;

ARTICLE; BIOPSY; CANCER STAGING; CLINICAL ARTICLE; COLORECTAL CARCINOMA; DNA DETERMINATION; DNA HYBRIDIZATION; DNA SEQUENCE; GENE AMPLIFICATION; HUMAN; HUMAN TISSUE; OLIGONUCLEOTIDE PROBE; ONCOGENE K RAS; POINT MUTATION; POLYMERASE CHAIN REACTION; TISSUE SECTION; TUMOR CELL LINE;

EID: 0030925091     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/43.6.936     Document Type: Article

References (29)

1. Burmer GC, Rabinovitch PS, Loeb LA. Analysis of c-Ki-ras mutations in human colon carcinoma by cell sorting, polymerase chain reaction, and sequencing. Cancer Res 1989;49:2141-6.
2. Fisher SG, Lerman LS. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory. Proc Natl Acad Sci U S A 1983;80:571-83.
3. Orita M, Iwakana H, Kanazawa H, Hayashi K, Sekiya T. Detection of polymorphisms of human DNA by gel electrophoresis as single strand conformation polymorphisms. Proc Natl Acad Sci U S A 1989;86:766-70.
4. Myers RM, Larin Z, Maniatis T. Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes. Science 1985;230:1242-6.
5. Bos JL, Verlaan de Vries M, Jansen AM, Veeneman GH, Van Boom JH, Van der Eb AJ. Three different mutations in the codon 61 of the human N-ras gene detected by synthetic oligonucleotide hybridization. Nucleic Acids Res 1984;12:9155-63.
6. Deng GA. Sensitive non-radioactive PCR-RFLP analysis for detecting point mutations at the 12th codon of oncogene c-Ha-ras in DNAs of gastric cancer. Nucleic Acids Res 1988;16:6231.
7. Wu DY, Ugozzoli L, Pal BK, Wallace RB. Allele-specific enzymatic amplification of β-globin genomic DNA for diagnosis of sickle cell anemia. Proc Natl Acad Sci U S A 1989;86:2757-60.
8. Levi SA, Urbano-lspizua A, Gill R, Thomas DM, Gilbertson J, Foster C, Marshall CJ. Multiple K-ras codon 12 mutations in cholangiocarcinomas demonstrated with a sensitive polymerase chain reaction technique. Cancer Res 1991;51:3497-502.
9. Kahn SM, Jiang W, Culbertson TA, Weinstein IB, Williams GM, Timita N, Ronai Z. Rapid detection and sensitive non radioactive detection of mutant K-ras genes via "enriched" PCR amplification. Oncogene 1991;6:1079-83.
10. Fearon ER. K-ras gene mutation as a pathogenetic and diagnostic marker in human cancer. J Natl Cancer Inst 1993;85:1978-80.
11. Finkelstein SD, Sayegh R, Christensen S, Swalsky PA. Genotypic classification of colorectal adenocarcinoma. Cancer 1993;71:3827-38.
12. Bos JL, Fearon ER, Hamilton SR, Verlaan-de Vries M, Van Boom JH, Van der Eb AJ, Vogelstein B. Prevalence of ras gene mutations in human colorectal cancer. Nature 1987;327:293-7.
13. Lopez-Crapez E, Chypre C, Marchand J, Grenier J. Detection of loss of heterozygozity by a tube-based assay. Biotechniques 1994;17:1072-6.
14. Meltzer SJ, Mane SM, Wood PK, Resau JH, Newkirk C, Terzakis JA. Activation of c-Ki-ras in gastrointestinal dysplasias determined by direct sequencing of polymerase chain reaction products. Cancer Res 1990;50:3627-30.
15. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989:9-16.
16. Roget A, Bazin H, Teoule R. Synthesis and use of labelled nucleoside phosphoramidite building blocks bearing a reporter group: biotinyl, dinitrophenyl, pyrenyl and dansyl. Nucleic Acids Res 1989;17:7643-51.
17. Erlich HA. PCR technology. Principles and applications for DNA amplification. New York: Stockton Press, 1989:63-6.
18. Sanger F, Nicklen S, Coulson AR. DNA sequencing with chainterminating inhibitors. Proc Natl Acad Sci U S A 1977;74:5463-70.
19. Kwok S, Higuchi R. Avoiding false positives with PCR. Nature 1989;339:237-8.
20. Iitia A, Mikola M, Gregersen N, Hurskainen P, Lövgren T. Detection of a point mutation using short oligonucleotide probes in allelespecific hybridization. Biotechniques 1994;17:566-73.
21. Melchior WB, Von Hippel PH. Alteration of the relative stability of dA.dT and dG.dC base pair in DNA. Proc Natl Acad Sci U S A 1973;70:298-302.
22. Pretlow TP, Brasitus TA, Fulton NC, Cheyer C, Kaplan EL. K-ras mutations in putative preneoplastic lesions in human colon. J Natl Cancer Inst 1993;85:2004-7.
23. Aoki Y, Lee JC, Pillai S, Isselbacher KT, Rustgi AK. Radiolabeled polymerase chain reaction assay for detection of ras oncogene point mutations in tumors. Clin Chem 1994;40:705-9.
24. Verlaan de Vries M, Boogaard ME, Van den Ebt M, Van Boom JM, Van der Eb AJ, Bos JL. A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotides. Gene 1986;50:313-20.
25. Zhang Y, Coyne MY, Will SG, Levinson CH, Kawasaki ES. Singlebase mutational analysis of cancer and genetic diseases using membrane bound modified oligonucleotides. Nucleic Acids Res 1991;19:3929-33.
26. Mills NE, Fishman CL, Rom WN, Dubin N, Jacobson DR. Increased prevalence of K-ras oncogene mutations in lung adenocarcinoma. Cancer Res 1995;55:1444-7.
27. Lo YMD, Mechal WZ, Fleming KA. False positive results and the polymerase chain reaction. Lancet 1988;ii:679.
28. Bos JL. Ras oncogene in human cancer: a review. Cancer Res 1989;49:4682-9.
29. Vogelstein B, Fearon ER, Hamilton SR. Genetic alterations during colorectal tumor development. N Engl J Med 1988;319:525-32.