Measurement of molecular beacons in the SPECTRAmax® GEMINI spectrofluorometer

Journal of Chemical Technology and Biotechnology

Volumn 75, Issue 10, 2000, Pages 942-944

  McGown, Evelyn ^a   Su, Michael ^a   Dennis, Richard ^a  

^a MOLECULAR DEVICES CORPORATION   (United States)

Author keywords

Fluorophore; Molecular beacons; Multiplexing; Oligonucleotides; PCR; Spectrofluorometry

Indexed keywords

DYES; FLUORESCENCE; FLUOROMETERS; MOLECULAR STRUCTURE; MONOCHROMATORS; SPECTROPHOTOMETRY;

DUAL SCANNING MONOCHROMATERS; FLUORUPHORES; MOLECULAR BEACONS; OLIGONUCLEOTIDES; SPECTROFLUOROMETRY;

NUCLEIC ACIDS;

OLIGONUCLEOTIDE;

ACCURACY; BIOMEDICAL TECHNOLOGY ASSESSMENT; CHEMICAL ANALYSIS; CONFERENCE PAPER; EXCITATION; GENERAL MEDICAL INSTRUMENTATION; MOLECULAR DYNAMICS; MOLECULAR RECOGNITION; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SENSIBILITY; SPECTRAL SENSITIVITY; SPECTROFLUOROMETRY;

EID: 0034307535     PISSN: 02682575     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-4660(200010)75:10<942::AID-JCTB310>3.0.CO;2-C     Document Type: Conference Paper

References (6)

1. Molecular beacons: Probes that fluoresce upon hybridisation
2. Multicolour molecular beacons for allele discrimination
3. Multiplex detection of single-nucleotide variations using molecular beacons
4. Thermodynamic basis of the enhanced specificity of structured DNA probes
5. Selecting excitation and emission wavelengths using the SPECTRAmax GEMINI microplate spectrofluorometer - Basic principles
6. Optimising excitation and emission wavelengths for narrow Stokes' shift fluorophores using the SPECTRAmax GEMINI microplate spectrofluorometer