Conformational epitopes on the diabetes autoantigen GAD65 identified by peptide phage display and molecular modeling

Journal of Immunology

Volumn 165, Issue 7, 2000, Pages 3830-3838

  Myers, M A ^a   Davies, J M ^a   Tong, J C ^a   Whisstock, J ^a   Scealy, M ^a   Mackay, I R ^a   Rowley, M J ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOANTIGEN; EPITOPE; GLUTAMATE DECARBOXYLASE; PEPTIDE LIBRARY; PYRIDOXAL 5 PHOSPHATE;

AMINO ACID SEQUENCE; ARTICLE; AUTOIMMUNE DISEASE; BINDING SITE; HUMAN; INSULIN DEPENDENT DIABETES MELLITUS; MOLECULAR MODEL; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

EID: 0034292407     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: 10.4049/jimmunol.165.7.3830     Document Type: Article

References (46)

1. r isoform of glutamic acid decarboxylase
2. r autoantibodies as predictors of insulin-dependent diabetes
3. Quantitative assay using recombinant human islet glutamic acid decarboxylase (GAD65) shows that 64K autoantibody positivity at onset predicts diabetes type
4. Glutamic acid decarboxylase (GAD) autoantibodies are additional predictive markers of type 1 (insulin-dependent) diabetes mellitus in high risk individuals
5. Antibodies to glutamic acid decarboxylase as predictors of insulin-dependent diabetes mellitus before clinical onset of disease
6. B lymphocytes are essential for the initiation of T cell-mediated autoimmune diabetes: Analysis of a new "speed congenic" stock of NOD.Ig μ null mice
7. B-cells are required for the initiation of insulitis and sialitis in nonobese diabetic mice
8. B lymphocytes are critical antigen-presenting cells for the initiation of T cell-mediated autoimmune diabetes in the nonobese diabetic mouse
9. Coxsackie B virus infection and onset of childhood diabetes
10. A prospective study of the role of coxsackie B and other enterovirus infections in the pathogenesis of IDDM
11. Diabetes mellitus due to viruses - Some recent developments
12. Diabetes induced by coxsackie virus: Initiation by bystander damage and not molecular mimicry
13. Autoimmunity to two forms of glutamate decarboxylase in insulin-dependent diabetes mellitus
14. Molecular mimicry in diabetes mellitus: The homologous domain in coxsackie B virus protein 2C and islet autoantigen GAD(65) is highly conserved in the coxsackie B-like enteroviruses and binds to the diabetes associated HLA-DR3 molecule
15. Cellular immunity to a determinant common to glutamate decarboxylase and coxsackie virus in insulin-dependent diabetes
16. Antibody cross-reactivity induced by the homologous regions in glutamic acid decarboxylase (GAD65) and 2C protein of coxsackievirus B4
17. Sequence homology of the diabetes-associated autoantigen glutamate decarboxylase with coxsackie B4-2C protein and heat shock protein 60 mediates no molecular mimicry of autoanfibodies
18. Identification of glutamic acid decarboxylase autoantibody heterogeneity and epitope regions in type I diabetes
19. Autoantigenic properties of native and denatured glutamic acid decarboxylase: Evidence for a conformational epitope
20. Human monoclonal islet cell antibodies from a patient with insulin-dependent diabetes mellitus reveal glutamate decarboxylase as the target antigen
21. Autoreactive epitopes defined by diabetes-associated human monoclonal antibodies are localized in the middle and C-terminal domains of the smaller form of glutamate decarboxylase
22. Immune reactivity of diabetes-associated human monoclonal autoantibodies defines multiple epitopes and detects two domain boundaries in glutamate decarboxylase
23. Epitope spreading and a varying but not disease-specific GAD65 antibody response in type 1 diabetes
24. Maturation of the humoural autoimmune response to epitopes of GAD in preclinical childhood type 1 diabetes
25. Structural motifs for pyridoxal-5′-phosphate binding in decarboxylases: An analysis based on the crystal structure of the Lactobacillus 30a omithine decarboxylase
26. Motifs and structural fold of the cofactor binding site of human glutamate decarboxylase
27. High-resolution autoreactive epitope mapping and structural modeling of the 65 kDa form of human glutamic acid decarboxylase
28. Mimotopes identified by phage display for the monoclonal antibody CII-CI to type II collagen
29. Evidence for somatic mutation and affinity maturation of diabetes associated human autoantibodies to glutamate decarboxylase
30. Characterization of the proteins purified with monoclonal antibodies to glutamic acid decarboxylase
31. Monoclonal antibodies to glutamic acid decarboxylase
32. Progressive sequence alignment as a prerequisite to correct phylogenetic tress
33. Predicting isomorphic residue replacements for protein design
34. Multiple alignment and sorting of peptides derived from phage-displayed random peptide libraries with polyclonal sera allows discrimination of relevant phagotopes
35. Selection of antibody ligands from a large library of oligopeptides expressed on a multivalent exposition vector
36. Structural constraints on the display of foreign peptides on filamentous bacteriophages
37. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positsion-specific gap penalties and weight matrix choice
38. Comparative protein modelling by satisfaction of spatial restraints
39. Pyridoxal-5′-phosphate, omithine decarboxylase, cofactor, decarboxylase, and crystallographic structure: Crystallographic structure of a PLP-dependent ornithine decarboxylase from lactobacillus 30a to 3.0 angstrom resolution
40. Autoantigenic reactivity of diabetes sera with a hybrid glutamic acid decarboxylase GAD67-65 molecule GAD67(1-101)/GAD65(96-585)
41. Evaluation of ICA512As in combination with other islet cell autoantibodies at the onset of IDDM
42. Identification of a dominant epitope of glutamic acid decarboxylase (GAD-65) recognized by autoantibodies in stiff-man syndrome
43. Comparative analysis of epitope recognition of glutamic acid decarboxylase (GAD) by autoantibodies from different autoimmune disorders
44. Diabetic sera react with the glutamic acid decarboxylase molecule in a dimeric-oligomeric form
45. GABA and glutamic acid decarboxylase (GAD): Gene to antigen to disease