Generation of monoclonal antibodies specifically directed against the proximal zinc finger of HIV type 1 NCp7

AIDS Research and Human Retroviruses

Volumn 16, Issue 13, 2000, Pages 1259-1267

  De Rocquigny, H ^a   Caneparo, A ^a   Dong, C Z ^a   Delaunay, T ^b   Roques, B P ^a  

^a CNRS   (France)

^b CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

MONOCLONAL ANTIBODY; ZINC FINGER PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIGEN ANTIBODY COMPLEX; ARTICLE; DNA STRAND; HUMAN IMMUNODEFICIENCY VIRUS 1; MOUSE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE;

EID: 0034284505     PISSN: 08892229     EISSN: None     Source Type: Journal    
DOI: 10.1089/08892220050117023     Document Type: Article

References (32)

1. Interactions of retroviral structural proteins with single-stranded nucleic acids
2. Potential metal-binding domains in nucleic acid binding proteins
3. Zinc binding to the HIV-1 nucleocapsid protein: A thermodynamic investigation by fluorescence spectroscopy
4. C-terminal retroviral-type zinc finger domain from the HIV-1 nucleocapsid protein is structurally similar to the N-terminal zinc finger domain
5. 1H NMR
6. Conformational behaviour of the active and inactive forms of the nucleocapsid NCp7 of HIV-1 studied by 1H NMR
7. Spatial proximity of the HIV-1 nucleocapsid protein zinc fingers investigated by time-resolved fluorescence and fluorescence resonance energy transfer
8. Dynamical behavior of the HIV-1 nucleocapsid protein
9. Structure, biological functions and inhibition of the HIV-1 proteins Vpr and NCp7
10. Structure of the complex between the HIV-1 nucleocapsid protein NCp7 and the single stranded pentanucleotide d(ACGCC)
11. Structure of the HIV-1 nucleocapsid protein bound to the SL3 psi-RNA recognition element
12. Small finger protein of avian and murine retroviruses has nucleic acid annealing activity and positions the replication primer tRNA onto genomic RNA
13. Viral RNA annealing activities of human immunodeficiency virus type 1 nucleocapsid protein require only peptide domains outside the zinc fingers
14. First glimpses at structure-function relationships of the nucleocapsid protein of retroviruses
15. Mutations in HIV reverse transcriptase which alter RNase H activity and decrease strand transfer efficiency are suppressed by HIV nucleocapsid protein
16. The annealing of the tRNA(Lys,3) to human immunodeficiency virus type 1 primer binding site is critically dependant on the NCp7 zinc fingers structure
17. Avian retroviral RNA encapsidation: Reexamination of functional 5' RNA sequences and the role of nucleocapsid Cys-His motifs
18. The Vpr protein of human immunodeficiency virus type 1 binds to nucleocapsid protein p7 in vitro
19. The zinc fingers of HIV nucleocapsid protein NCp7 direct interactions with the viral regulatory protein Vpr
20. Direct activation of protein phosphatase-2A0 by HIV-1 encoded protein complex NCp7:vpr
21. Interaction with the p6 domain of the Gag precursor mediates incorporation into virions of Vpr and Vpx proteins from primate lentiviruses
22. HIV-1, Vpr and the cell cycle
23. Involvement of HIV-1 nucleocapsid protein in the recruitment of reverse transcriptase into nucleoprotein complex form in vitro
24. Evidence of interactions between the nucleocapsid protein NCp7 and the reverse transcriptase of HIV-1
25. Monoclonal antibody-mediated inhibition of RNA binding and annealing activities of HIV type 1 nucleocapsid protein
26. Conformational changes between human immunodeficiency virus type I nucleocapsid protein NCp7 and its precursor NCpl5 as detected by anti-NCp7 monoclonal antibodies
27. Synthesis and conformational studies of a cyclic analogue of the proximal zinc finger of HIV-1 NCp7 for antibody generation
28. Replacement of His-23 by Cys in a zinc finger of HIV-1 NCp7 led to a change in 1H NMR derived in 3D structure and a loss of biological activity
29. Sequence requirements for encapsidation of deletion mutants and chimeras of human immunodeficiency virus type 1 Gag precursor into retrovirus-like particles
30. Use of heterologous expression systems to study retroviral morphogenesis
31. First large scale chemical synthesis of the 72 amino acid HIV-1 nucleocapsid protein NCp7 in an active form
32. Three-dimensional 1H NMR structure of the nucleocapsid protein NCp10 of Moloney murine leukemia virus