Binding characteristics of CebR, the regulator of the ceb operon required for cellobiose/cellotriose uptake in Streptomyces reticuli

FEMS Microbiology Letters

Volumn 190, Issue 1, 2000, Pages 127-132

  Schlösser, Andreas ^a   Aldekamp, Torsten ^a   Schrempf, Hildgund ^a  

^a UNIVERSITY OF OSNABRÜCK   (Germany)

Author keywords

Actinomycete; CebR regulator; Cellobiose cellotriose ABC transporter; Streptomycete

Indexed keywords

CARBOHYDRATE DERIVATIVE; CELLOBIOSE; CELLOTRIOSE; DNA BINDING PROTEIN; HISTIDINE; HYBRID PROTEIN; PROTEIN CEBR; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; CARBOHYDRATE TRANSPORT; CONTROLLED STUDY; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; OPERON; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN PURIFICATION; PROTEIN SECRETION; STREPTOMYCES; STREPTOMYCES RETICULI;

ATP-BINDING CASSETTE TRANSPORTERS; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; CELLOBIOSE; CELLULOSE; CLONING, MOLECULAR; DNA, BACTERIAL; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REGULATOR; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; OPERON; RECOMBINANT FUSION PROTEINS; STREPTOMYCES;

EID: 0034284025     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00304-9     Document Type: Article

References (16)

1. Wachinger, G., Bronnenmeier, K., Staudenbauer, W.L. and Schrempf, H. (1989) Identification of mycelium-associated cellulase from Streptomyces reticuli. Appl. Environ. Microbiol. 55, 2653-2657.
2. Schlochtermeier, A., Niemeyer, F. and Schrempf, H. (1992) Biochemical and electron microscopic studies of the Streptomyces reticuli cellulase (Avicelase) in its mycelium-associated and extracellular forms. Appl. Environ. Microbiol. 58, 3240-3248.
3. Schlochtermeier, A., Walter, S., Schröder, J., Moormann, M. and Schrempf, H. (1992) The gene encoding the cellulase (Avicelase) Cell from Streptomyces reticuli and analysis of protein domains. Mol. Microbiol. 6, 3611-3621.
4. Walter, S. and Schrempf, H. (1996) The synthesis of the Streptomyces reticuli cellulase (Avicelase) is regulated by both activation and repression. Mol. Gen. Genet. 251, 186-195.
5. Schlösser, A. and Schrempf, H. (1996) A lipid-anchored binding protein is a component of an ATP-dependent cellobiose/-triose transport system from the cellulose degrader Streptomyces reticuli. Eur. J. Biochem. 242, 332-338.
6. Schlösser, A., Jantos, J., Hackmann, K. and Schrempf, H. (1999) Characterization of the binding protein-dependent cellobiose and cellotriose transport system of the cellulose degrader Streptomyces reticuli. Appl. Environ. Microbiol. 65, 2636-2643.
7. Schlösser, A., Kampers, T. and Schrempf, H. (1997) The Streptomyces ATP-binding component Msik assists in cellobiose and maltose transport. J. Bacteriol. 179, 2092-2095.
8. Weickert, M.J. and Adhya, S. (1992) A family of bacterial regulators homologous to Gal and Lac repressors. J. Biol. Chem. 267, 15869-15874.
9. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
10. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Smith, J.A., Seidman, J.G. and Struhl, K. (1987) Current Protocols in Molecular Biology. Greene Publishing Associates, New York.
11. Studier, F.W. and Moffatt, B.A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113-130.
12. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
13. Choy, H.E., Park, S.W., Parrack, P. and Adhya, S. (1995) Transcription regulation by inflexibility of promoter DNA in a looped complex. Proc. Natl. Acad. Sci. USA 92, 7327-7331.
14. Spiridonov, N.A. and Wilson, D.B. (1999) Characterization and cloning of CelR, a transcriptional regulator of cellulase genes from Thermomonospora fusca. J. Biol. Chem. 274, 13127-13132.
15. Lewis, M., Chang, G., Horton, N.C., Kercher, M.A., Pace, H.C., Schumacher, M.A., Brennan, R.G. and Lu, P. (1996) Crystal structure of the lactose operon repressor and its complexes with DNA and inducer. Science 271, 1247-1254.
16. Schumacher, M.A., Choi, K.Y., Zalkin, H. and Brennan, R.G. (1994) Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by α helices. Science 266, 763-770.