Nuclear-labeling index analysis (NLIA), a software package used to perform accurate automation of cell nuclear-labeling index analysis on immunohistochemically stained rat liver samples

Computer Methods and Programs in Biomedicine

Volumn 63, Issue 1, 2000, Pages 55-70

  Xu, Yi Hua ^a   Sattler, Gerald L ^a   Edwards, Harlene ^a   Pitot, Henry C ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Adobe Photoshop; Automation; Autoradiography; Color image; Image analysis; Immunohistochemistry; NIH image; Nuclear labeling index analysis; Scion Image

Indexed keywords

AUTOMATIC INDEXING; AUTOMATION; CELL CULTURE; COLOR IMAGE PROCESSING; COMPUTER AIDED ANALYSIS; COMPUTER SOFTWARE; IMAGE ANALYSIS; IMMUNOLOGY;

AUTORADIOGRAPHY; IMMUNOHISTOCHEMISTRY; NUCLEAR-LABELING INDEX; SOFTWARE PACKAGE NLIA;

MEDICAL IMAGING;

ANIMAL CELL; ANTIBODY LABELING; ARTICLE; AUTOMATION; CELL COUNT; CELL CULTURE; CELL DEATH; CELL NUCLEUS; COMPUTER PROGRAM; CONTROLLED STUDY; IMAGE ANALYSIS; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; LABELING INDEX; NONHUMAN; RAT; TECHNIQUE;

EID: 0034254554     PISSN: 01692607     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0169-2607(00)00075-4     Document Type: Article

References (26)

1. R.I. Freshney, Culture of the Animal Cells. A Manual of Basic Technique’ Alan R. Liss, New York, (1987).
2. T.L. Goldsworthy R. Fransson-Steen R.R. Maronpot Importance of and approaches to quantification of hepatocyte apoptosis Toxicol. Pathol. 24 1 1996 24 35
3. M.A. Perricone V. Saldate D.M. Hyde Quantitation of fibroblast population growth rate in situ using computerized image analysis Microsc. Res. Tech. 31 3 1995 257 264
4. A. Faranda R. Silvestrini S. Canova A. Costa Image analysis for the evaluation of p53 expression in human cancers Anal. Cell. Pathol. 11 2 1996 107 113
5. I. Camby I. Salmon A. Danguy J.L. Pasteels R. Kiss The use of the digital cell image analysis of Feulgen-stained nuclei to detect apoptosis Histochem. Cell Biol. 104 5 1995 407 414
6. M. Ide M. Jimbo M. Yamamoto O. Kubo Tumor cell counting using an image analysis program for MIB-1 immunohistochemistry Neurol. Med. Chir. (Tokyo) 37 2 1997 158 162
7. P.N. Furness L. Rogers-Wheatley K.P. Harris Semiautomatic quantitation of macrophages in human renal biopsy specimens in proteinuric states J. Clin. Pathol. 50 2 1997 118 122
8. K.M. Lam A simple and fast way to count heterophils in chemotaxis Avian Dis. 42 4 1998 812 814
9. S.K. Bisland B. Calvieri S. Doyle J. Gariepy Computer quantification of intracellular gold particles Biotechniques 26 3 1999 494 500
10. NIH Image home page at http://rsb.info.nih.gov/nih-image/
11. Links to image analysis related sites at http://rsb.info.nih.gov/nih-image/links.html
12. SPCC, SigmaScan Pro. version 4.0 for Windows at http://www.spss.com/software/science/SigmaScan
13. UTHSCSA ImageTool home page at http://ddsdx.uthscsa.edu/dig/itdesc.html
14. Scion Corporation home page at http://www.scioncorp.com/
15. Y.-h Xu H.C. Pitot Building quantitative stereology data files with Scion Image, a public domain image processing and analysis software Comput. Methods Programs Biomed. 59 2 1999 131 142
16. Pitot Laboratory web page at http://mcardle.oncology.wisc.edu/pitot/
17. Microsoft Corporation, Visual Basic on line for Visual Basic 6.0 professional Version, CD material.
18. Scion Corporation, image instruction menu. This article can be found in ScnImage.pdf file, which comes with Scion Image setup package.
19. Scion Corporation, inside Scion Image for Windows. This article can be found in Inside.pdf file which comes with Scion Image setup package.
20. B.L. Kreamer Use of a low-speed, iso-density Percoll centrifugation method to increase the viability of isolated rat hepatocyte preparations In Vitro Cell. Dev. Biol. 22 4 1986 201 211
21. Y.P. Dragan J. Hully R. Crow M. Mass H.C. Pitot Incorporation of bromodeoxyuridine in glutathione S -transferase-positive hepatocytes during rat multistage hepatocarcinogenesis Carcinogenesis 15 9 1994 1939 1947
22. H. Hikita E.F. Nuwaysir J. Vaughan K. Babcock M.J. Haas Y.P. Dragan H.C. Pitot The effect of short-term fasting, phenobarbital and refeeding on apoptotic loss, cell replication and gene expression in rat liver during the promotion stage Carcinogenesis 19 8 1998 1417 1425
23. Y. Gavrieli Y. Sherman S.A. Ben-Sasson Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation J. Cell Biol. 119 3 1992 493 501
24. K. Binder, T. Alspach, H. Ehrenfeld, et al., Selections, Paths, and Masks. Photoshop 4 Complete, Hayden Books, Indianapolis, 1997, pp 131–183.
25. A.C. Ruifrok Quantification of immunohistochemical staining by color translation and automated thresholding Anal. Quant. Cytol. Histol. 19 2 1997 107 113
26. P. Ranefall L. Egevad B. Nordin E. Bengtsson A new method for segmentation of colour images applied to immunohistochemically stained cell nuclei Anal. Cell. Pathol. 15 3 1997 145 156