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0343779045
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note
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The absence of the amide I band and the presence of a strong amide II band suggest that the carbonyl groups of the amides are oriented parallel to the surface and that the amides are involved in hydrogen bonding with nearby carbonyl groups of either a carboxylic acid or an amide.
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0343779042
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note
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co) = 0.95 after 10 min.
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43
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0343779043
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note
-
SPR is an optical technique that detects refractive index changes at the surface of a thin film of metal. The adsorption of protein to a SAM on gold is an example of the interactions that can be measured in situ by SPR.
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0343779044
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note
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2OH are similar; we could not measure the adsorption of lysozyme at pH ≥ 11 (such that it would be negatively charged) because the manufacturer of our SPR instrument (Biacore) does not recommend exposing the instrument to pH > 10 for extended periods of time.
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