Fatty acid determination in chicken egg yolk: A comparison of different methods

Poultry Science

Volumn 79, Issue 8, 2000, Pages 1168-1171

  Wang, Y ^a   Sunwoo, H ^a   Cherian, G ^a,b   Sim, J S ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b OREGON STATE UNIVERSITY   (United States)

Author keywords

Egg yolk; Fatty acid assay

Indexed keywords

CHLOROFORM; FATTY ACID; METHANOL; SAPONIN;

ANIMAL; ARTICLE; CHEMISTRY; CHICKEN; COMPARATIVE STUDY; EGG YOLK; GAS CHROMATOGRAPHY; METHODOLOGY; METHYLATION;

ANIMALS; CHICKENS; CHLOROFORM; CHROMATOGRAPHY, GAS; EGG YOLK; FATTY ACIDS; METHANOL; METHYLATION; SAPONINS;

EID: 0034241488     PISSN: 00325791     EISSN: None     Source Type: Journal    
DOI: 10.1093/ps/79.8.1168     Document Type: Article

References (14)

1. Ajuyah, A. O., R. T. Hardin, and J. S. Sim, 1993. Effect of dietary full-fat flaxseed with and without antioxidant on the fatty acid composition of major lipid classes of chicken meats. Poultry Sci. 72:125-136.
2. Caston, L., and S. Leeson, 1990. Dietary flax and egg composition. Poultry Sci. 69:1617-1620.
3. Cherian, G., R. T. Wolfe, and J. S. Sim, 1996. Dietary oils with added tocopherols: Effects on egg or tissue tocopherols, fatty acids and oxidative stability. Poultry Sci. 75:423-431.
4. Drucker, D. B., 1972. The identification of streptococci by gas liquid chromatography. Microbios 5:109-112.
5. Farrell, D., 1998. Enrichment of chicken eggs with n-3 polyunsaturated fatty acids and evaluation of enriched eggs in humans. Am. J. Clin. Nutr. 68:538-544.
6. Folch, J., M. Lees, and G. H. Sloane-Stanely, 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-507.
7. Lepage, G., and C. C. Roy, 1986. Direct transesterification of all classes of lipids in a one step reaction. J. Lipids Res. 27:114-120.
8. Marshall, A. C., K. S. Kubena, K. R. Hinton, P. S. Hargis, and M. E. Van Elswyk, 1994. N-3 fatty acid enriched table eggs: a survey of consumer acceptability. Poultry Sci 73:1334-1340.
9. Myers, J. L., and A. D. Well, 1995. Research design and statistical analysis. Lawrence Erlbaum Associates, Hillsdale, NJ.
10. Outen, G. E., D. E. Beever, and J. S. Fenlon, 1975. Direct methylation of long chain fatty acid in feeds, digesta and feces without prior extraction. J. Sci. Feed Agric. 27:419-425.
11. Rodriguez-Palmero, M., M. C. Lopez-Sabater, A. I. Castellote-Bargallo, M. C. De La Torre-Boronat, and M. Rivero-Urgell, 1997. Comparison of two methods for the determination of fatty acid profile in plasma and erythrocytes. J. Chromatogr. A. 778:435-139.
12. SAS, 1990. User's Guide. Version 6.06 ed. SAS Institute Inc., Cary, NC.
13. 3/methanol transesterification of lyophilized samples instead of Folch extraction gives higher yields. Anal. Biochem. 198:184-190.
14. Van Elswyk, M. E., 1998. Egg as a functional food alternative to fish and supplements for the consumption of docosahexaenoic acid. Page 20 in: Second International Symposium on Egg Nutrition and Newly Emerging Ovo Biotechnologies. Banff, AB, Canada. (Abstr.).