A high-throughput homogeneous assay for reverse transcriptase using generic reagents and time-resolved fluorescence detection

Analytical Biochemistry

Volumn 281, Issue 2, 2000, Pages 182-186

  Zhang, Ji Hu ^a   Chen, Tongming ^a   Nguyen, Son H ^a   Oldenburg, Kevin R ^a  

^a DUPONT COMPANY   (United States)

Author keywords

Homogeneous assay; HTRF; Reverse transcriptase; Time resolved fluorescence

Indexed keywords

PROTEINS; REAGENTS;

HIGH-THROUGHPUT SCREENS; HIV REVERSE TRANSCRIPTASE; HOMOGENEOUS ASSAYS; HOMOGENEOUS TIME-RESOLVED FLUORESCENCE ASSAYS; HTRF; REVERSE TRANSCRIPTASES; SIGNAL-TO-BACKGROUND RATIO; TIME-RESOLVED FLUORESCENCE;

FLUORESCENCE;

ALLOPHYCOCYANIN; EUROPIUM; REAGENT; RNA DIRECTED DNA POLYMERASE; RNA DIRECTED DNA POLYMERASE INHIBITOR; STREPTAVIDIN; URIDINE TRIPHOSPHATE;

ARTICLE; BINDING KINETICS; ENZYME ACTIVITY; ENZYME ASSAY; FLUORESCENCE; METHODOLOGY; PRIORITY JOURNAL; TITRIMETRY;

RNA VIRUSES;

EID: 0034214349     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4567     Document Type: Article

References (20)

1. Goff, S. P. (1990) J. Acquir. Immune Defic. Syndr. 3, 817-831.
2. Merluzzi, V. J., et al. (1990) Science 250, 1411-1413.
3. Hoffman, A. D., Banapour, B., and Levy, J. A. (1985) Virology 147, 326-335.
4. Neumüller, M., Karlstrüm, A. R., Källander, C. F. R., and Gronowitz, J. S. (1990) Biotechnol. Appl. Biochem. 12, 34-56.
5. Lemaitre, M., et al. (1992) Antiviral Res. 17(Suppl. 1), 48.
6. Nocito, M., Villar, L. M., and González-Porqué, P. (1993) Anal. Biochem. 208, 206-208.
7. Suzuki, K., Craddock, B. P., and Steigbigel, R. T. (1993) Anal. Biochem. 210, 277-281.
8. Urabe, T., et al. (1992) J. Virol. Methods 40, 145-154.
9. Müller, B., Restle, T., Reinstein, J., and Goody, R. S. (1991) Biochemistry 30, 3709-3715.
10. Suzuki, K., Craddock, B. P., Kano, T., and Steigbigel, R. T. (1993) J. Virol. Methods 41, 21-28.
11. Cook, R. F., Cook, S. J., and Issel, C. J. (1992) Biotechniques 13, 380-386.
12. Oldenburg, K. R. (1998) in Annual Reports in Medical Chemistry (Bristol, J. A., Ed.), Vol. 33, pp. 301-311, Academic Press, New York.
13. Kolb, A. J. (Ed.) (1997) Part III. Assay technologies and detection methods, in High Throughput Screening: The Discovery of Bioactive Substances (Devilin, J. P., Ed.), pp. 275-456, Dekker, New York.
14. Mathis, G. (1995) Clin. Chem. 41, 1391.
15. Alpha-Bazin, B., and Mathis, G. (1998) The Society of Biomolecular Screening 4th Annual Conference, Sept. 21-24, Baltimore, MD. [poster presentation]
16. Reardon, J. E. (1993) J. Biol. Chem. 268, 8743-8751.
17. Furman, P. A., Painter, G., Wilson, J. E., Cheng, N., and Hopkins, S. (1991) Proc. Natl. Acad. Sci. USA 88, 6013-6017.
18. Kolb, J. M., Yamanada, G., and Manly, S. P. (1997) in High Throughput Screening: The Discovery of Bioactive Substances (Devilin, J. P., Ed.), pp. 377-388, Dekker, New York.
19. Zhang, J.-H., Chung, T. D. Y., and Oldenburg, K. R. (1999) J. Biomol. Screen. 4, 67-73.
20. Corbett, J. W., et al. (1999) Antimicrob. Agents Chemother. 43, 2893-2897.