Oxalomalate, a competitive inhibitor of aconitase, modulates the RNA-binding activity of iron-regulatory proteins

Biochemical Journal

Volumn 348, Issue 2, 2000, Pages 315-320

  Festa, Michela ^a   Colonna, Alfredo ^a   Pietropaolo, Concetta ^a   Ruffo, Alfredo ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

hydroxy oxalosuccinic acid; Iron metabolism; RNA binding protein

Indexed keywords

ACONITATE HYDRATASE; ENZYME INHIBITOR; GLYOXYLIC ACID; IRON REGULATORY FACTOR; MERCAPTOETHANOL; OXALOACETIC ACID; OXALOMALATE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL LYSATE; COMPETITIVE INHIBITION; ELECTROPHORETIC MOBILITY; FIBROBLAST; HUMAN; HUMAN CELL; IRON METABOLISM; LIVER; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN RNA BINDING;

3T3 CELLS; ACONITATE HYDRATASE; ANIMALS; CYTOSOL; ENZYME INHIBITORS; GLYOXYLATES; HUMANS; IRON REGULATORY PROTEIN 1; IRON REGULATORY PROTEIN 2; IRON-REGULATORY PROTEINS; IRON-SULFUR PROTEINS; KINETICS; LIVER; MERCAPTOETHANOL; MICE; OXALATES; OXALOACETATE; PROTEIN ISOFORMS; RNA-BINDING PROTEINS; TUMOR CELLS, CULTURED;

ANIMALIA;

EID: 0034212588     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3480315     Document Type: Article

References (28)

1. 1 Ruffo, A., Romano, M. and Adinolfi, A. (1959) Inhibition of aconitase by glyoxylate plus oxaloacetate. Biochem. J. 72, 613-618
2. 2 Ruffo, A., Testa, E , Adinolfi, A. and Pelizza, G. (1962) Control of the citric acid cycle by glyoxylate. 1. A new inhibitor of aconitase formed by the condensation of glyoxylate with oxaloacetale. Biochem. J. 85, 588-593
3. 3 Ruffo, A , Adinolfi, A., Budillon, G. and Capobianco, G. (1962) Control of the citric acid cycle by glyoxylate 2 Mechanism of the inhibition of respiration in liver and kidney particles. Biochem. J. 85, 593-596
4. 4 Ruffo, A., Testa, E., Adinolfi, A., Pelizza, G. and Moratti, R. (1967) Control of the citric acid cycle by glyoxylate. Mechanism of the inhibition by oxalomalate and γ-hydroxy-α-oxoglutarate. Biochem. J. 103, 19-23
5. 5 Adinolfi, A., Moratti, R., Olezza, S. and Ruffo, A. (1969) Control of the citric acid cycle by glyoxylate. The mechanism of inhibition of oxoglutarate dehydrogenase, isocitrate dehydrogenase and aconitate hydratase. Biochem. J 114, 513-518
6. 6 Adinolfi, A., Guarriera-Bobyleva, V., Olezza, S. and Ruffo, A. (1971) Inhibition by oxalomalate of rat liver mitochondrial and extramitochondrial aconitate hydratase Biochem. J. 125, 557-562
7. 7 Adinolfi, A., Speranza, M. L., Guarriera-Bobyleva, V and Ruffo, A. (1973) Citrate metabolism in liver of rats treated with glyoxylate and oxaloacetate. Ital. J. Biochem. 22, 92-104
8. 8 Klausner, R. D., Rouault, T. A. and Harford, J. B. (1993) Regulating the fate of mRNA: the conlrol of cellular iron metabolism. Cell 72, 19-28
9. 9 Melefors, Ö. and Hentze, M. W. (1993) Iron regulatory factor - the conductor of cellular iron regulation. Blood Rev. 7, 251-258
10. 10 Hentze, M. W. and Kühn, L. C. (1996) Molecular control of vertebrate iron metabolism: mRNA-based regulatory circuits operated by iron, nitric oxide, and oxidative stress. Proc. Natl. Acad. Sci U.S.A 93, 8175-8182
11. 11 Rouault, T. A. and Klausner, R. D. (1996) Iron-sulfur clusters as biosensors of oxidants and iron. Trends Biochem. Sci. 21, 174-177
12. 12 Eisenstein, R. S. and Blemings, K. P. (1998) Iron regulatory proteins, iron responsive elements and iron homeostasis. J. Nutr. 128, 2295-2298
13. 13 Theil, E. C. (1998) The iron responsive element (IRE) family of mRNA regulators. In Metal lons in Biological Systems, vol. 35 (Sigel, A. and Sigel, H., eds.), pp. 403-434, Marcel Dekker, New York
14. 14 Festa, M , Colonna, A., Pietropaolo, C. and Ruffo, A. (1999) Regulation of iron metabolism by oxalomalate. Rend Fis. Acc. Lincei s 9, 10, 21-24
15. 15 Guo, B , Yu, Y. and Leibold, E. A. (1994) Iron regulates cytoplasmic levels of a novel iron responsive element-binding protein without aconitase activity J Biol. Chem. 269, 24252-24260
16. 16 Paraskeva, E and Hentze, M W (1996) Iron-sulphur clusters as genetic regulatory switches: the bifunctional iron regulatory protein-1. FEBS Lett. 389, 40-43
17. 17 Rouault, T. A., Stout, C. D., Kaptain, S., Harford, J. B and Klausner, R D. (1991) Structural relationship between an iron-regulated RNA-binding protein (IRE-BP) and aconitase functional implications Cell 64, 881-883
18. 18 Philpott, C. C., Haile, D., Rouault, T. A. and Klausner, R. D. (1993) Modification of a free Fe-S cluster cysteine residue in the active iron-responsive element-binding protein prevents RNA binding. J. Biol. Chem. 268, 17655-17658
19. 19 Hirling, H., Henderson, B R. and Kühn, L C. (1994) Mutational analysis of the [4Fe-4S]-cluster converting iron regulatory factor from its RNA-binding form to cytoplasmic aconitase. EMBO J 13, 453-461
20. 20 Kennedy, M. C., Emptage, M. H., Dreyer, J L. and Beinert, H. (1983) The role of iron in the activation-inactivation of aconitase. J Biol. Chem. 258, 11098-11105
21. 21 Haile, D. J., Rouault, T A., Harford, J. B., Kennedy, M. C., Blondin, G. A., Beinert, H. and Klausner, R. D. (1992) Cellular regulation of the iron-responsive element binding protein: disassembly of the cubane iron-sulphur cluster results in high-affinity RNA binding. Proc. Natl. Acad. Sci. U.S.A. 89, 11735-11739
22. 22 Guo, B., Brown, F. M., Phillips, J. D., Yu, Y. and Leibold, E. A. (1995) Characterization and expression of iron regulatory protein 2 (IRP2). J. Biol. Chem. 270, 16529-16535
23. 23 Iwai, K., Klausner, R. D. and Rouault, T. A. (1995) Requirements for iron-regulated degradation of the RNA binding protein, iron regulatory protein 2. EMBO J. 14, 5350-5357
24. 24 Recalcati, S., Conte, D. and Cairo, G. (1999) Preferential activation of iron regulatory protein-2 in cell lines as a result of higher sensitivity to iron Eur. J. Biochem. 259, 304-309
25. 25 Hentze, M. W., Rouault, T. A., Harford, J. B. and Klausner, R. D. (1989) Oxidation-reduction and the molecular mechanism of a regulatory RNA-protein interaction. Science 244, 357-359
26. 26 Guo, B., Phillips, J. D., Yu, Y. and Leibold, E. A. (1995) Iron regulates the intracellular degradation of iron regulatory protein 2 by the proteasome. J. Biol. Chem 270, 21645-21651
27. 27 Henderson, B. R. and Kühn, L. C. (1995) Differential modulation of the RNA-binding proteins IRP-1 and IRP-2 in response to iron. J. Biol. Chem. 270, 20509-20515
28. 28 Philpott, C. C., Klausner, R. D. and Rouault, T. A. (1994) The bifunctional iron-responsive element binding protein/cytosolic aconitase: the role of active-site residues in ligand binding and regulation. Proc. Natl. Acad. Sci. U.S.A. 91, 7321-7325