Isolation and sequencing of a cDNA clone encoding a 20-kDa protein with trypsin inhibitory activity

Bioscience, Biotechnology and Biochemistry

Volumn 64, Issue 6, 2000, Pages 1305-1309

  Ashida, Yoshiyuki ^a   Matsushima, Akihito ^a   Tsuru, Yukari ^a   Hirota, Tomoko ^a   Hirata, Toshifumi ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Glycine max (soybean); Kunitz type trypsin inhibitor; Leguminosae; Molecular cloning

Indexed keywords

FABACEAE; GLYCINE MAX;

COMPLEMENTARY DNA; PLANT DNA; SOYBEAN TRYPSIN INHIBITOR; TRYPSIN INHIBITOR;

AMINO ACID SEQUENCE; ARTICLE; COMPARATIVE STUDY; GENE; GENE EXPRESSION; GENETICS; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY; SOYBEAN;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DNA, PLANT; GENE EXPRESSION; GENES, PLANT; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, AMINO ACID; SOYBEANS; TRYPSIN INHIBITOR, KUNITZ SOYBEAN; TRYPSIN INHIBITORS;

EID: 0034203507     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.64.1305     Document Type: Article

References (14)

1. c. J. Biochem., 98, 435-448 (1985).
2. Jofuku, K. D. and Goldberg, R. B., Kunitz trypsinin hibitor genes are differentially expressed during the soybean life cycle and in transformed tobacco plants. Plant Cell, 1, 1079-1093 (1989).
3. Jofuku, K. D., Schipper, R. D., and Goldberg, R. B., A frameshift mutation prevents Kunitz trypsin inhibitor mRNA accumulation in soybean embryos. Plant Cell, 1, 427-435 (1989).
4. trypsin inhibitors (Kunitz type). Plant Physiol., 101,1401-1402(1993).
5. Hubu, Y., Fukushima, H., Sakata, Y., Abe, H., and Funada, R., A gene encoding a major Kunitz proteinase inhibitor of storage organs of winged bean is also expressed in the phloem of stems. Plant Mol. Biol., 32, 1209-1213 (1996).
6. Hirata, T., Izumi, S., and Tsuji, S., A 20-kDa protein with the GTP-binding and trypsin inhibitory activities from Glycine max. Biosci. Biotechnol. Biochem., 63, 1816-1818 (1999).
7. Thompson, J. D., Higgins, D. G., and Gibson, T. J., CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res., 22, 4673-4680 (1994).
8. Shure, M., Wessler, S., and Fedoroff, N., Molecular identification and isolation of the Waxy locus in maize. Cell, 35, 225-233 (1983).
9. Liu, Y. G., Mitsukawa, N., Oosumi, T., and Whittier, R. F., Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J., 8, 457-463 (1995).
10. Hall, A., The cellular functions of small GTP-binding proteins. Science, 249, 635-640 (1990).
11. Terryn, N., Montagu, M. V., and Inze, D., GTP-binding proteins in plants. Plant Mol. Biol., 22, 143-152 (1993).
12. Sprang, S. R., G protein mechanisms: insights from structural analysis. Annu. Rev. Biochem., 66, 639-678 (1997).
13. Mulligan, R. M., Chory, J., and Ecker, J. R., Signaling in plants. Proc. Natl. Acad. Sci. USA, 94, 2793-2795 (1997).
14. Welham, T., O’Neill, M., Johnson, S., and Domoney, W. C., Expression patterns of genes encoding seed trypsin inhibitors in Pisum sativum. Plant Sci., 131, 13-24 (1998).