Serotype classification and characterisation of the rotavirus SA11 VP6 protein using mass spectrometry and two-dimensional gel electrophoresis

Functional and Integrative Genomics

Volumn 1, Issue 1, 2000, Pages 12-24

  Emslie, Kerry R ^a,c   Molloy, Mark P ^a   Barardi, Celia R M ^a   Jardine, Daniel ^a   Wilkins, Marc R ^a,d   Bellamy, A Richard ^b   Williams, Keith L ^a,d  

^a MACQUARIE UNIVERSITY   (Australia)

^b UNIVERSITY OF AUCKLAND   (New Zealand)

^c NATIONAL HEALTH AND MEDICAL RESEARCH COUNCIL   (Australia)

^d Proteome Systems Ltd   (Australia)

Author keywords

Acetylation; Deamidation; Modifications; Peptide mass fingerprinting; Rotavirus; Serotype classification

Indexed keywords

AMIDE; CAPSID PROTEIN; ISOPROTEIN; PEPTIDE; SA 11 ANTIGEN, ROTAVIRUS; SA-11 ANTIGEN, ROTAVIRUS; TRYPSIN; VIRUS ANTIGEN; VP6 PROTEIN, ROTAVIRUS;

ACETYLATION; AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CLASSIFICATION; GENETICS; ISOELECTRIC POINT; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PROTEIN PROCESSING; ROTAVIRUS; SEROTYPING; TWO DIMENSIONAL GEL ELECTROPHORESIS; VIRUS CAPSID;

ACETYLATION; AMIDES; AMINO ACID SEQUENCE; ANTIGENS, VIRAL; CAPSID; CAPSID PROTEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ISOELECTRIC POINT; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN ISOFORMS; PROTEIN PROCESSING, POST-TRANSLATIONAL; ROTAVIRUS; SEROTYPING; TRYPSIN;

EID: 0034191018     PISSN: 1438793X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s101420000002     Document Type: Article

References (20)

1. Annan RS, Carr SA (1996) Phosphopeptide analysis by matrix-assisted laser desorption time-of-flight mass spectrometry. Anal Chem 68: 3413-3421
2. Biemann K (1990) Sequencing of peptides by tandem mass spectrometry and high-energy collision-induced dissociation. In: McCloskey JA (ed) Methods in enzymology, mass spectrometry, vol 193. Academic Press, San Diego, pp 455-479
3. Bischoff R, Kolbe HVJ (1994) Deamidation of asparagine and glutamine residues in proteins and peptides: structural determinants and analytical methodology. J Chromatogr B Biomed Sci Appl 662: 261-278
4. Both GW, Siegman LJ, Bellamy AR, Ikegami N, Shatkin AJ, Furuichi Y (1984) Comparative sequence analysis of rotavirus genomic segment 6 -the gene specifying viral subgroups 1 and 2. J Virol 51: 97-101
5. Brass VJ, Hagelstein A, Gerhardt E, Galle PR (1996) Myristylation of the large surface protein is required for hepatitis B virus in vitro infectivity. Virology 218: 396-399
6. Clark B, Desselberger U (1988) Myristylation of rotavirus proteins. J Gen Virol 69: 2681-2686
7. Estes MK, Mason BB, Crawford S, Cohen J (1984) Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein. Nucleic Acids Res 12: 1875-1887
8. Gorziglia M, Larrea C, Liprandi F, Esparza J (1985) Biochemical evidence for the oligomeric (possibly trimeric) structure of the major inner capsid polypeptide (45 K) of rotaviruses. J Gen Virol 66: 1889-1900
9. Herbert BR, Molloy MP, Gooley AA, Walsh BJ, Bryson WG, Williams KL (1998) Improved protein solubility in 2-D electrophoresis using tributyl phosphine. Electrophoresis 19: 845-851
10. Hoshino Y, Kapikian AZ (1994) Rotavirus antigens. In: Ramig RD (ed) Current topics in microbiology and immunology. Rotaviruses. Springer, Berlin Heidelberg New York, pp 179-227
11. Kabcenell AK, Atkinson PH (1985) Processing of the rough endoplasmic reticulum membrane glycoproteins of rotavirus SA11. J Cell Biol 101: 1270-1280
12. Matsudaira P (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262: 10035-10038
13. Patton JT (1994) Rotavirus replication. In: Ramig RF (ed) Current topics in microbiology and immunology. Rotaviruses. Springer, Berlin Heidelberg New York, pp 107-127
14. Rabilloud T, Valette C, Lawrence JJ (1994) Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilised pH gradients in the first dimension. Electrophoresis 15: 1552-1558
15. Rabilloud T, Adessi C, Giraudel A, Lunardi J (1997) Improvement of the solubilisation of proteins in two-dimensional electrophoresis with immobilised pH gradients. Electrophoresis 18: 307-316
16. Smith RE, Kister SE, Carozzi NB (1989) Cloning and expression of the major inner capsid protein of SA-11 simian rotavirus in E. coli. Gene 79: 239-248
17. Street JE, Croxson MC, Chadderton WF, Bellamy AR (1982) Sequence diversity of human rotavirus strains investigated by Northern blot hybridization analysis. J Virol 43: 369-378
18. Tang B, Gilbert JM, Matsui SM, Greenberg HB (1997) Comparison of the rotavirus gene 6 from different species by sequence analysis and localization of subgroup-specific epitopes using site-directed mutagenesis. Virology 237: 89-96
19. Wilkins MR, Lindskog I, Gasteiger E, Bairoch A, Sanchez J-C, Hochstrasser DF, Appel RD (1997) Detailed peptide characterisation using PEPTIDEMASS - a World-Wide Web accessible tool. Electrophoresis 18: 403-408
20. Wilkins MR, Gasteiger E, Gooley AA, Herbert BR, Molloy MP, Binz PA, Ou K, Sanchez J-C, Bairoch A, Williams KL, Hochstrasser DF (1999) Large-scale mass spectrometric discovery of protein post-translational modifications. J Mol Biol 289: 645-657