CFTR intron I increases luciferase expression driven by CFTR 5'-flanking DNA in a yeast artificial chromosome

Genomics

Volumn 64, Issue 2, 2000, Pages 211-215

  Mogayzel Jr , Peter J ^a,b   Ashlock, Melissa A ^b  

^a DEPARTMENT OF PATHOLOGY   (United States)

^b NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LUCIFERASE; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA FLANKING REGION; DNA RESPONSIVE ELEMENT; ENZYME ACTIVITY; ENZYME INDUCTION; GENE AMPLIFICATION; GENE FUNCTION; INTRON; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REPORTER GENE; YEAST ARTIFICIAL CHROMOSOME; ANIMAL; BIOSYNTHESIS; CELL CLONE; CHO CELL; GENE DOSAGE; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; GENETICS; HAMSTER; METABOLISM; PHYSIOLOGY; PROMOTER REGION; SOUTHERN BLOTTING; UPREGULATION;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

ANIMALS; BLOTTING, SOUTHERN; CHO CELLS; CHROMOSOMES, ARTIFICIAL, YEAST; CLONE CELLS; CRICETINAE; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; GENE DOSAGE; GENE EXPRESSION REGULATION; GENES, REPORTER; INTRONS; LUCIFERASES; PROMOTER REGIONS (GENETICS); TRANSFECTION; UP-REGULATION;

EID: 0034152828     PISSN: 08887543     EISSN: None     Source Type: Journal    
DOI: 10.1006/geno.2000.6119     Document Type: Article

References (34)

1. Asselbergs F. A. M., Grossenbacher R., Ortmann R., Hengerer B., McMaster G. K., Sutter E., Widmer R., Buxton F. Position-independent expression of a human nerve growth factor-luciferase reporter gene cloned on a yeast artificial chromosome vector. Nucleic Acids Res. 26:1998;1826-1833.
2. Breuer W., Kartner N., Riordan J. R., Cabantchik Z. I. Induction of expression of the cystic fibrosis transmembrane conductance regulator. J. Biol. Chem. 267:1992;10465-10469.
3. Chan R. Y., Boudreau-Larivière C., Angus L. M., Mankal F. A., Jasmin B. J. An intronic enhancer containing an N-box motif is required for synapse- and tissue-specific expression of the acetylcholinesterase gene in skeletal muscle fibers. Proc. Natl. Acad. Sci. USA. 96:1999;4627-4632.
4. Cheng S. H., Fang S. L., Zabner J., Marshall J., Piraino S., Schiavi S. C., Jefferson D. M., Welsh M. J., Smith A. E. Functional activation of the cystic fibrosis trafficking mutant ΔF508-CFTR by overexpression. Am. J. Physiol. 268:1995;L615-L624.
5. Choi T. K., Hollenbach P. W., Pearson B. E., Ueda R. M., Weddell G. N., Kurahara C. G., Woodhouse C. S., Kay R. M., Loring J. F. Transgenic mice containing a human heavy chain immunoglobulin gene fragment in a yeast artificial chromosome. Nat. Genet. 4:1993;117-123.
6. Chou J.-L., Rozmahel R., Tsui L.-C. Characterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene. J. Biol. Chem. 266:1991;24471-24476.
7. Green E. D., Olson M. V. Chromosomal region of the cystic fibrosis gene in yeast artificial chromosomes: A model for human genome mapping. Science. 250:1990;94-98.
8. Hirata H., Yamamura I., Yasuda K., Kobayashi A., Tada N., Suzuki M., Hirayoshi K., Hosokawa N., Nagata K. Separate cis-acting DNA elements control cell type- and tissue-specific expression of collagen binding molecular chaperone HSP47. J. Biol. Chem. 274:1999;35703-35710.
9. Huxley C. Transfer of YACs to mammalian cells and transgenic mice. Setlow J. K., Genetic Engineering. 1994;65-91 Plenum, New York.
10. Huxley C. Exploring gene function: Use of yeast artificial chromosome transgenesis. Methods. 14:1998;199-210.
11. Huxley C., Hagino Y., Schlessinger D., Olson M. V. The human HPRT gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusion. Genomics. 9:1991;742-750.
12. Kappel A., Rönicke V., Damert A., Flamme I., Risau W., Breier G. Identification of vascular endothelial growth factor (VEGF) receptor-2 (Flk-1) promoter/enhancer sequences sufficient for angioblast and endothelial cell-specific transcription in transgenic mice. Blood. 93:1999;4284-4292.
13. Koh J., Sferra T. J., Collins F. S. Characterization of the cystic fibrosis transmembrane conductance regulator promoter region. J. Biol. Chem. 268:1993;15912-15921.
14. Lewis B. C., Shah N. P., Braun B. S., Denny C. T. Creation of a yeast artificial chromosome fragmentation vector based on lysine-2. Genet. Anal. Tech. Appl. 9:1992;86-90.
15. Li S., Moy L., Pittman N., Shue G., Aufiero B., Neufeld E. J., LeLeiko N. S., Walsh M. J. Transcriptional repression of the cystic fibrosis transmembrane conductance regulator gene, mediated by CCAAT displacement protein/cut homolog, is associated with histone deacetylation. J. Biol. Chem. 274:1999;7803-7815.
16. Mansoura M. K., Henning K. A., Compton S. T., Ashlock M. A. Expression of a full-length, uniquely-identifiable CFTR gene from a 610 KB YAC stably introduced into a CF airway epithelial cell line. Pediatr. Pulmonol. Suppl. 17:1998;216.
17. Matthews R. P., McKnight G. S. Characterization of the cAMP response element of the cystic fibrosis transmembrane conductance regulator gene promoter. J. Biol. Chem. 271:1996;31869-31877.
18. McDonald R. A., Matthews R. P., Idzerda R. L., McKnight G. S. Basal expression of the cystic fibrosis transmembrane conductance regulator gene is dependent on protein kinase A activity. Proc. Natl. Acad. Sci. USA. 92:1995;7560-7564.
19. Mogayzel P. J. Jr., Henning K. A., Bittner M. L., Novotny E. A., Schwiebert E. M., Guggino W. B., Jiang Y., Rosenfeld M. A. Functional human CFTR produced by stable Chinese hamster ovary cell lines derived using yeast artificial chromosomes. Hum. Mol. Genet. 6:1997;59-68.
20. Moulin D. S., Manson A. L., Nuthall H. N., Smith D. J., Huxley C., Harris A. In vivo analysis of DNase I hypersensitive sites in the human CFTR gene. Mol. Med. 5:1999;211-223.
21. Murray R. C., Williams F. M. R., Flintoff W. F. Structural organization of the reduced folate carrier gene in Chinese hamster ovary cells. J. Biol. Chem. 271:1996;19174-19179.
22. Nuthall H. N., Moulin D. S., Huxley C., Harris A. Analysis of DNase-1-hypersensitive sites at the 3′ end of the cystic fibrosis transmembrane conductance regulator gene (CFTR). Biochem. J. 341:1999;601-611.
23. Peterson K. R. Production and analysis of transgenic mice containing yeast artificial chromosomes. Setlow J. K., Genetic Engineering. 1997;235-255 Plenum, New York.
24. Pittman N., Shue G., LeLeiko N. S., Walsh M. J. Transcription of cystic fibrosis transmembrane conductance regulator requires a CCAAT-like element for both basal and cAMP-mediated regulation. J. Biol. Chem. 270:1995;28848-28857.
25. Riordan J. R., Rommens J. M., Kerem B., Alon N., Rozmahel R., Grzelczak Z., Zielenski J., Lok S., Plavsic N., Chou J.-L., Drumm M. L., Iannuzzi M. C., Collins F. S., Tsui L.-C. Identification of the cystic fibrosis gene: Cloning and characterization of complementary DNA. Science. 245:1989;1066-1073.
26. Rommens J. M., Iannuzzi M. C., Kerem B., Drumm M. L., Melmer G., Dean M., Rozmahel R., Cole J. L., Kennedy D., Hidaka N., Zsiga M., Buchwald M., Riordan J. R., Tsui L.-C., Collins F. S. Identification of the cystic fibrosis gene: Chromosome walking and jumping. Science. 245:1989;1059-1065.
27. Shen B.-Q., Barthelson R. A., Skach W., Gruenert D. C., Sigal E., Mrsny R. J., Widdicombe J. H. Mechanism of inhibition of cAMP-dependent epithelial chloride secretion by phorbol esters. J. Biol. Chem. 268:1993;19070-19075.
28. Smith A. N., Barth M. L., McDowell T. L., Moulin D. S., Nuthall H. N., Hollingsworth M. A., Harris A. A regulatory element in intron 1 of the cystic fibrosis transmembrane conductance regulator gene. J. Biol. Chem. 271:1996;9947-9954.
29. Smith A. N., Wardle C. J. C., Harris A. Characterization of DNase I hypersensitive sites in the 120kB 5′ to the CFTR gene. Biochem. Biophys. Res. Commun. 211:1995;274-281.
30. Trapnell B. C., Zeitlin P. L., Chu C.-S., Yoshimura K., Nakamura H., Guggino W. B., Bargon J., Banks T. C., Dalemans W., Pavirani A., Lecocq J.-P., Crystal R. G. Down-regulation of cystic fibrosis gene mRNA transcript levels and induction of the cystic fibrosis secretory phenotype in epithelial cells by phorbol ester. J. Biol. Chem. 266:1991;10319-10323.
31. Tybulewicz V. L. J., Crawford C. E., Jackson P. K., Bronson R. T., Mulligan R. C. Neonatal lethality and lymphopenia in mice with a homozygous disruption of the c-abl proto-oncogene. Cell. 65:1991;1153-1163.
32. Vassaux G., Manson A. L., Huxley C. Copy number-dependent expression of a YAC-cloned human CFTR gene in a human epithelial cell line. Gene Ther. 4:1997;618-623.
33. Yoshimura K., Nakamura H., Trapnell B. C., Dalemans W., Pavirani A., Lecocq J.-P., Crystal R. G. The cystic fibrosis gene has a "housekeeping"-type promoter and is expressed at low levels in cells of epithelial origin. J. Biol. Chem. 266:1991;9140-9144.
34. Zielenski J., Rozmahel R., Bozon D., Kerem B., Grzelczak Z., Riordan J. R., Rommens J., Tsui L.-C. Genomic DNA sequence of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Genomics. 10:1991;214-228.