Functional human CFTR produced by stable Chinese hamster ovary cell lines derived using yeast artificial chromosomes

Human Molecular Genetics

Volumn 6, Issue 1, 1997, Pages 59-68

  Mogayzel Jr , Peter J ^a,b   Henning, Karla A ^a   Bittner, Michael L ^a   Novotny, Elizabeth A ^a   Schwiebert, Erik M ^b   Guggino, William B ^b   Jiang, Yuan ^a   Rosenfeld, Melissa A ^a  

^a NATIONAL HUMAN GENOME RESEARCH INSTITUTE   (United States)

^b DEPARTMENT OF PATHOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC AMP; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL CELL; ARTICLE; CELL LINE; CHO CELL; FLUORESCENCE IN SITU HYBRIDIZATION; GENE CONTROL; GENE EXPRESSION; HUMAN; NONHUMAN; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SOUTHERN BLOTTING; YEAST ARTIFICIAL CHROMOSOME;

ANIMALS; CHO CELLS; CHROMOSOMES, ARTIFICIAL, YEAST; CRICETINAE; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; GENE EXPRESSION; HUMANS; RNA, MESSENGER; TRANSFECTION;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; MAMMALIA;

EID: 8044248972     PISSN: 09646906     EISSN: None     Source Type: Journal    
DOI: 10.1093/hmg/6.1.59     Document Type: Article

References (51)

1. Welsh, M.J., Tsui, L.-C., Boat, T.F. and Beaudet, A.L. (1995). In Scriver, C.R., Beaudet, A.L., Sly, W.S. and Valle, D. (eds.), The Metabolic and Molecular Bases of Inherited Disease. McGraw-Hill, New York, Vol. 3, pp. 3799-3876.
2. Welsh, M.J. and Liedtke, C.M. (1986) Chloride and potassium channels in cystic fibrosis airway epithelia. Nature. 322, 467-470.
3. Widdicombe, J.H. (1986) Cystic fibrosis and β-adrenergic response of airway epithelial cell cultures. Am. J. Physiol., 251, R818-R822.
4. Frizzell, R.A., Rechkemmer, G. and Shoemaker, R.L. (1986) Altered regulation of airway epithelial cell chloride channels in cystic fibrosis. Science, 233, 558-560.
5. Stutts, M.J., Canessa, C.M., Olsen, J.C., Hamrick, M., Cohn, J.A., Rossier, B.C. and Boucher, R.C. (1995) CFTR as a cAMP-dependent regulator of sodium channels. Science, 269, 847-850.
6. Schwiebert, E.M., Egan, M.E., Hwang, T.-H., Fulmer, S.B., Allen, S.S., Cutting, G.R. and Guggino, W.B. (1995) CFTR regulates outwardly rectifying chloride channels through an autocrine mechanism involving ATP. Cell, 81, 1063-1073.
7. Cystic Fibrosis Mutation Database, http://genet.sickkids.on.ca.
8. Kerem, B.-S., Rommens, J.M., Buchanan, J.A., Markiewicz, D., Cox, T.K., Chakravarti, A., Buchwald, M. and Tsui, L.-C. (1989) Identification of the cystic fibrosis gene: Genetic anaylsis. Science, 245, 1073-1080.
9. Trezise, A.E.O., Linder, C.C., Grieger, D., Thompson, E.W., Meunier, H., Griswold, M.D. and Buchwald, M. (1993) CFTR expression is regulated during both the cycle of the seminiferous epithelium and the oestrous cycle of rodents. Nature Genet., 3, 157-164.
10. Trapnell, B.C., Chu, C.-S., Paakko, P.K., Banks, T.C., Yoshimura, K., Ferrans, V.J., Chernick, M.S. and Crystal, R.G. (1991) Expression of the cystic fibrosis transmembrane conductance regulator gene in the respiratory tract of normal individuals and individuals with cystic fibrosis. Proc. Natl. Acad. Sci. USA. 88, 6565-6569.
11. Riordan, J.R., Rommens, J.M., Kerem, B.-s., Alon, N., Rozmahel, R., Grzelczak, Z., Zielenski, J., Lok, S., Plavsic, N., Chou, J.-L., Drumm, M.L., Iannuzzi, M.C., Collins, F.S. and Tsui, L.-C (1989) Identification of the cystic fibrosis gene: Cloning and characterization of complementary DNA. Science, 245, 1066-1073.
12. Engelhardt, J.F., Yankaskas, J.R., Ernst, S.A., Yang, Y., Marino, C.R., Boucher, R.C., Cohn, J.A. and Wilson, J.M. (1992) Submucosal glands are the predominant site of CFTR expression in the human bronchus. Nature Genet., 2, 240-248.
13. Yoshimura, K., Nakamura, H., Trapnell, B.C., Dalemans, W., Pavirani, A., Lecocq, J.-P. and Crystal, R.G. (1991) The cystic fibrosis gene has a 'housekeeping'-type promoter and is expressed at low levels in cells of epithelial origin. J. Biol. Chem., 266, 9140-9144.
14. Koh, J., Sferra, T.J. and Collins, F.S. (1993) Characterization of the cystic fibrosis transmembrane conductance regulator promoter region. J. Biol. Chem., 268, 15912-15921.
15. Chou, J.-L., Rozmahel, R. and Tsui, L.-C. (1991) Characterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene. J. Biol. Chem., 266, 24471-24476.
16. Denamur, E. and Chehab, F.F. (1994) Analysis of the mouse and rat CFTR promoter regions. Hum. Mol. Genet., 3, 1089-1094.
17. Breuer, W., Kartner, N., Riordan, J.R. and Cabantchik, Z.I. (1992) Induction of expression of the cystic fibrosis transmembrane conductance regulator. J. Biol. Chem., 267, 10465-10469.
18. Trapnell, B.C., Zeitlin, P.L., Chu, C.-S., Yoshimura, K., Nakamura, H., Guggino, W.B., Bargon, J., Banks, T.C., Dalemans, W., Pavirani, A., Lecocq, J.-P. and Crystal, R.G. (1991) Down-regulation of cystic fibrosis gene mRNA transcript levels and induction of the cystic fibrosis secretory phenotype in epithelial cells by phorbol ester. J. Biol. Chem., 266, 10319-10323.
19. Shen, B.-Q., Barthelson, R.A., Skach, W., Gruenert, D.C., Sigal, E., Mrsny, R.J. and Widdicombe, J.H. (1993) Mechanism of inhibition of cAMP-dependent epithelial chloride secretion by phorbol esters. J. Biol. Chem., 268, 19070-19075.
20. McDonald, R., Matthews, R.P., Idzerda, R.L. and McKnight, G.S. (1995) Basal expression of the cystic fibrosis transmembrane conductance regulator gene is dependent on protein kinase A activity. J. Biol. Chem., 92, 7560-7564.
21. Pittman, N., Shue, G., LeLeiko, N.S. and Walsh, M.J. (1995) Transcription of cystic fibrosis transmembrane conductance regulator requires a CCAAT-like element for both basal and cAMP-mediated regulation. J. Biol. Chem., 270, 28848-28857.
22. - secretion in colonic epithelial cells expressing high levels of cystic fibrosis transmembrane conductance regulator. J. Biol. Chem., 267, 5575-5583.
23. Smith, A.N., Luzia Barth, M., McDowell, T.L., Moulin, D.S., Nuthall, H.N., Hollingsworth, M.A. and Harris, A. (1996) A regulatory element in intron 1 of the cystic fibrosis transmembrane conductance regulator gene. J. Biol. Chem., 271, 9947-9954.
24. Smith, A.N., Wardle, C.J.C. and Harris, A. (1995) Charcterization of DNase I hypersensitive sites in the 120kB 5′ to the CFTR gene. Biochem. Biophys. Res. Commun., 211, 274-281.
25. Anand, R., Ogilvie, D.J., Butler, R., Riley, J.H., Finniear, R.S., Powell, S.J., Smith, J.C. and Markham, A.F. (1991) A yeast artificial chromosome contig encompassing the cystic fibrosis locus. Genomics, 9, 124-130.
26. Green, E.D. and Olson, M.V. (1990) Chromosomal region of the cystic fibrosis gene in yeast artificial chromosomes: A model for human genome mapping. Science, 250, 94-98.
27. Rommens, J.M., Iannuzzi, M.C., Kerem, B.-S., Drumm, M.L., Melmer, G., Dean, M., Rozmahel, R., Cole, J.L., Kennedy, D., Hidaka, N., Zsiga, M., Buchwald, M., Riordan, J.R., Tsui, L.-C. and Collins, F.S. (1989) Identification of the cystic fibrosis gene: Chromosome walking and jumping. Science, 245, 1059-1065.
28. Florijn, R.J., Bonden, L.A.J., Vrolijk, H., Wiegant, J., Vaandrager, J.-W., Baas, F., Dunnen, J.T.d., Tanke, H.J., Ommen, G.-J.B.v. and Raap, A.K. (1995) High-resolution DNA Fiber-FISH for genomic DNA mapping and colour bar-coding of large genes. Hum. Mol. Genet., 4, 831-836.
29. Rosenberg, C., Florijn, R.J., Rijke, F.M.V.D., Blonden, L.A.J., Raap, T.K., Ommen, G.-J.B.V. and Dunnen, J.T.D. (1995) High resolution DNA Fiber-fish on yeast artifical chromosomes: Direct visualization of DNA replication. Nature Genet., 10, 477-479.
30. Riley, J.H., Morten, J.E.N. and Anand, R. (1992) Targeted integration of neomycin into yeast artificial chromosomes (YACs) for transfection into mammalian cells. Nucleic Acids Res., 20, 2971-2976.
31. Rosenfeld, M.A., Rosenfeld, S.J., Danel, C., Banks, T.C. and Crystal, R.G. (1994) Increasing expression of the normal human CFTR cDNA in cystic fibrosis epithelial cells results in a progressive increase in the level of CFTR protein expression, but a limit on the level of cAMP-stimulated chloride secretion. Hum. Gene Ther., 5, 1121-1129.
32. Choi, T.K., Hollenbach, P.W., Pearson, B.E., Ueda, R.M., Weddell, G.N., Kurahara, C.G., Woodhouse, C.S., Kay, R.M. and Loring, J.F. (1993) Transgenic mice containing a human heavy chain immunoglobulin gene fragment in a yeast artificial chromosome. Nature Genet., 4, 117-123.
33. Strauss, W.M. and Jaenisch, R. (1992) Molecular complementation of a collagen mutation in mammalian cells using yeast artificial chromosomes. EMBO J., 11, 417-422.
34. D'Urso, M., Zucchi, I., Ciccodicola, A., Palmieri, G., Abidi, F.E. and Schlessinger, D. (1990) Human glucose-6-phosphate dehydrogenase gene carried on a yeast artificial chromosome encodes active enzyme activity in monkey cells. Genamics, 7, 531-534.
35. Huxley, C. (1994). In Setlow, J.K. (ed.) Genetic Engineering. Plenum Press, New York, Vol. 16, pp. 65-91.
36. - channel in CHO cells stably expressing the cystic fibrosis gene. Nature, 352, 628-631.
37. Gabriel, S.E., Price, E.M., Boucher, R.C. and Stuns, M.J. (1992) Small linear chloride channels are endogenous to nonepithelial cells. Am. J. Physiol., 263, C708-C713.
38. Kusaba, H., Kohno, K., Asakuno, K., Kuwano, M., Okumura, K., Green, E.D., Schlessinger, D. and Wada, M. (1995) Functional expression of yeast artificial chromosome-human multidrug resistance genes in mouse cells. Genome Res., 5, 245-258.
39. Peterson, K.R., Zitnik, G., Huxley, C., Lowrey, C.H., Gnirke, A., Leppig, K.A., Papayannopoulou, T. and Stamatoyannapoulos, G. (1993) Use of yeast artificial chromosomes (YACs) for studying control of gene expression: Correct regulation of the gene of a human β-globin locus YAC following transfer into mouse erythroleukemia cell lines. Proc. Natl. Acad. Sci. USA, 90, 11207-11211.
40. Huxley, C., Hagino, Y., Schlessinger, D. and Olson, M.V. (1991) The human HPRT gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusion. Genomics, 9, 742-750.
41. Cook, J.R., Emanuel, S.L., Donnelly, R.J., Soh, J., Mariano, T.M., Schwartz, B., Rhee, S. and Pestka, S. (1994) Sublocalization of the human interferon-γ receptor accessory factor gene and characterization of accessory factor activity by yeast artificial chromosome fragmentation. J. Biol. Chem., 269, 7013-7018.
42. Durbin, H., Young, S., Stewart, L.M., Wrba, F., Rowan, A.J., Snary, D. and Bodmer, W.F. (1994) An epitope on carcinoembryonic antigen defined by the clinically relevant antibody PR1A3. Proc. Natl. Acad. Sci. USA, 91, 4313-4317.
43. Anderson, M.P., Rich, D.P., Gregory, R.J., Smith, A.E. and Welsh, M. (1991) Generation of cAMP-activated chloride currents by expression of CFTR. Science, 251, 679-682.
44. Spencer, F., Ketner, G., Connelly, C. and Hieter, P. (1993) Targeted recombination-based cloning and manipulation of large DNA segments in yeast. Methods: A Companion to Methods in Enzymology, 5, 161-175.
45. Markie, D., Ragoussis, J., Senger, G., Rowan, A., Sansom, D., Trowsdale, J., Sheer, D. and Bodmer, W.F. (1993) New vector for transfer of yeast artificial chromosomes to mammalian cells. Som. Cell Mol. Genet., 19, 161-169.
46. Zielenski, J., Rozmahel, R., Bozon, D., Kerem, B.-S., Grzelczak, Z., Riordan, J.R., Rommens, J. and Tsui, L.-C. (1991) Genomic DNA sequence of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Genomics, 10, 214-228.
47. Rosenfeld, M.A., Yoshimura, K., Trapnell, B.C., Yoneyama, K., Rosenthal, E.R., Dalemans, W., Fukayama, M., Bargon, J., Stier, L.E., Stratford-Perricaudet, L., Perricaudet, M., Guggino, W.B., Pavirani, A., Lecocq, J.-P. and Crystal, R.G. (1992) In vivo transfer of the human cystic fibrosis transmembrane conductance regulator gene to the airway epithelium. Cell, 68, 143-155.
48. Pinkel, D., Straume, T. and Gray, J.W. (1986) Cytogenetic analysis using quanitative, high sensitivity, fluoresence hybridization. Proc. Natl. Acad. Sci. USA, 83, 2934-2938.
49. Heiskanen, M., Karhu, R., Hellsten, E., Peltonen, L., Kallioniemi, O.P. and Palotie, A. (1994) High resolution mapping using fluorescence in situ hybridization to extended DNA fibers prepared from agarose-embedded cells. BioTechniques, 17, 928-933.
50. Cheng, S.H., Gregory, R.J., Marshall, J., Paul, S., Souza, D.W., White, G.A., O'Riordan, C.R. and Smith, A.E. (1990) Defective intracellular transport and processing of CFTR is the molecular basis of most cystic fibrosis. Cell, 63, 827-834.
51. Fulmer, S.B., Schwiebert, E.M., Morales, M.M., Guggino, W.B. and Cutting, G.R. (1995) Two cystic fibrosis transmembrane conductance regulator mutations have different effects on both pulmonary phenotype and regulation of outwardly rectfied chloride currents. Proc. Natl. Acad. Sci. USA, 92, 6832-6836.